ISSN:
1573-4927
Keywords:
hemoglobin
;
type I
;
type IV
;
trout (Salmo L. typicus)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract We have analyzed the hemoglobins of five individual trout from the Ohrid Lake (Salmo L. typicus) by electrophoretic methods, by reversed-phase high-performance liquid chromatography, and by limited structural analyses. The two major classes of hemoglobin are type I (35% of total) and type IV (65%). Type IV is the major oxygen-transporting hemoglobin; it consists of three types of β chain (in about equal quantities) and three types of α chain (one major and two minor types). Several structural differences have been observed between these three β(IV) chains and between the three α(IV) chains, suggesting a complex genetic system governing the synthesis of these proteins. Moreover, a few amino acid substitutions occur at positions involved in contacts between chains, which suggests that differences in oxygen affinity may exist between these various type IV hemoglobins. Type I hemoglobin is less complex because it contains one type of β chain and two α chains; the latter two differ in numerous positions, suggesting duplications of the α(I)-globin gene. The α and β chains of type I hemoglobin differ considerably from the α and β chains of type IV hemoglobin, indicating the existence of α(I)- and β(I)-globin genes separate from the α(IV)- and β(IV)- globin genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00569329
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