Publication Date:
2013-01-01
Description:
Background. Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems.Objective. To detect urinary metabolomics after hepatic ischemia/reperfusion (I/R) injury induced by the Pringle maneuver using gas chromatography-mass spectrometry (GC-MS).Methods. Male Sprague-Dawley rats () were randomly divided into 4 groups (/group): sham operation, day 1, day 3, and day 5. Rats in the day 1, day 3, and day 5 groups underwent the Pringle maneuver. Serum alanine transaminase (ALT) and total bilirubin (TBIL) were measured, and hematoxylin and eosin (HE) staining of the liver tissue was performed. GC-MS was used to detect urinary metabolomics.Results. Compared with the sham group, the serum ALT and TBIL levels at day 1 were significantly elevated () and then decreased and reached close to normal levels at day 5. GC-MS detected 7 metabolites which had similar changes as those of liver tissue revealed by histological examination. Significant differences in lactic acid, pyruvic acid, alanine, serine, and glycerol-3-phosphate were found among the groups (). Principle component analysis showed that 7 metabolites distinguished the day 1 and day 3 groups from the sham group.Conclusions. Noninvasive urinary metabolomic analysis is a potential means for the early detection and diagnosis of hepatic I/R injury.
Print ISSN:
0278-0240
Electronic ISSN:
1875-8630
Topics:
Biology
,
Chemistry and Pharmacology
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