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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Chromosoma 25 (1968), S. 104-106 
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The occurrence of a synaptinemal complex in yeast was demonstrated by an electron-microscopical examination of meiotic prophase I. The complex is formed during the interval between the maximum of meiotic DNA synthesis and the first division.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 144 (1986), S. 272-278 
    ISSN: 1432-072X
    Keywords: Arthrobacter P1 ; Methylamine ; Formaldehyde ; RuMP cycle of formaldehyde fixation ; Regulation ; Carbon catabolite repression ; Continuous culture ; Transient states
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The regulation of methylamine and formaldehyde metabolism in Arthrobacter P1 was investigated in carbonlimited continuous cultures. To avoid toxic effects of higher formaldehyde concentrations, formaldehyde-limited cultures were established in smooth substrate transitions from choline-limitation. Evidence was obtained that the synthesis of enzymes involved in the conversion of methylamine into formaldehyde and in formaldehyde fixation is induced sequentially in this organism. Compared to growth with methylamine the molar growth yield on formaldehyde was approximately 30% higher. This difference is mainly due to the expenditure of energy for the uptake of methylamine from the medium. The addition of a pulse of a “heterotrophic” substrate, glucose or acetate, to C1 substrate-limited continuous cultures resulted in relief of carbon limitation and transient synthesis of increasing amounts of cell material. Concomitantly, a significant decrease in the specific activities of hexulose phosphate synthase was observed. However, the total activity of hexulose phosphate synthase in these cultures remained clearly in excess of that required to fix the formaldehyde that became available in time. The observed strong decrease in the specific activities of this RuMP cycle enzyme strongly suggests that its synthesis is controlled via catabolite repression exerted by the metabolism of “heterotrophic” substrates.
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  • 3
    ISSN: 1432-072X
    Keywords: Xanthobacter ; Taxonomy ; Methylotrophs ; Methanol ; Calvin cycle ; Carbon dioxide fixation ; RuBisC/O ; Regulation ; Continuous culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract All Xanthobacter strains studied are versatile autotrophic bacteria, able to grow on methanol and other substrates. Strain 25a, a yellow-pigmented, pleomorphic, Gram-negative bacterium, capable of autotrophic growth on methanol, formate, thiosulfate, and molecular hydrogen, was isolated from an enrichment culture inoculated with soil from a subtropical greenhouse. Subsequent studies showed that the organism also grows on a wide range of multicarbon substrates. Ammonia, nitrate and molecular nitrogen were used as nitrogen sources. The taxonomic relationship of strains H4-14 and 25a with previously described Xanthobacter strains was studied by numerical classification. Strain H4-14 was identified as a X. flavus strain, but the precise position of strain 25a remained uncertain. It probably belongs to a new species of the genus Xanthobacter. The levels of various enzymes involved in autotrophic and heterotrophic metabolism were determined following growth of strains H4-14 and 25a in batch and continuous cultures. The mechanisms involved in controlling ribulose-1,5-bisphosphate carboxylase/oxygenase synthesis in Xanthobacter strains appear to be comparable to those observed for other autotrophic bacteria, namely repression by organic compounds and derepression by autotrophic energy sources, such as methanol and hydrogen.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 129 (1981), S. 47-48 
    ISSN: 1432-072X
    Keywords: Adenylate energy charge ; Phosphate ; Saccharomyces ; Sporulation ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The induction of sporulation in yeast is generally accompanied by a sharp increase in energy metabolism which is evidenced by a rise of the adenylate energy charge by that time. The energy charge can be held at a low level by limitation of the phosphate supply in the growth medium. Ascus formation remains unaffected by this treatment. This suggests that the rise in ATP production normally encountered during early sporulation is not essential for the initiation of sporulation.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 102 (1975), S. 117-122 
    ISSN: 1432-072X
    Keywords: Actinomycin ; Allomyces ; Gametogenesis ; Nuclear Cap ; Polyribosomes ; RNA Synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Synchronous gametogenesis in the water mold Allomyces arbuscula is blocked by actinomycin D added at the onset of the process. Formation of the male gametangium can be selectively inhibited by administering actinomycin one hr after the induction of gametogenesis. The polyribosome pattern obtained after density gradient centrifugation remains virtually unchanged throughout gametogenesis until a stage immediately preceding maturation of the gametes. When ribosomes from gametes and swarming zygotes are analyzed on gradients, some RNase-sensitive material is found to band in the heavier portion of the gradient. Its presence suggests that some messenger RNA associated with ribosomes is conserved in the swarming cells. During gametogenesis RNA is de novo synthesized and becomes associated with the polyribosomes.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 144 (1986), S. 279-285 
    ISSN: 1432-072X
    Keywords: Arthrobacter P1 ; Methylamine ; Form-aldehyde ; RuMP cycle of formaldehyde fixation ; Regulation ; Induction ; Carbon catabolite repression ; Resting cell suspensions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The inducing potential of formaldehyde on the synthesis of hexulose phosphate synthase, a key enzyme of the RuMP cycle in Arthrobacter P1, was investigated in resting cell suspensions. Induction of this enzyme only occurred at formaldehyde concentrations of 0.5 mM and below. No evidence was obtained for the involvement of an inactivation-activation mechanisms that controlled the activity of existing hexulose phosphate synthase molecules. Addition of formaldehyde at a rate of 1 mmol·l-1·h-1 to cells of Arthrobacter P1 growing in batch culture on the “heterotrophic” substrates glucose or acetate resulted in the immediate and very rapid synthesis of hexulose phosphate synthase. These results, obtained under carbon excess conditions, clearly show that induction by formaldehyde is the overriding control mechanism in the regulation of the synthesis of this enzyme in Arthrobacter P1. The regulation of the synthesis of this key enzyme of the RuMP cycle of formaldehyde fixation in Arthrobacter P1 (induction by formaldehyde) is therefore completely different from that generally observed for the enzymes of the RuBP cycle of CO2 fixation in facultatively autotrophic bacteria (repression/ derepression mechanism). Addition of methylamine at a rate of 1 mmol·l-1·h-1 to batch cultures growing on “heterotrophic” substrates resulted in accumulation of the C1 substrate, but not in repression of the synthesis of amine oxidase. The slow start of methylamine utilization in cells growing on glucose suggests that methylamine either is a relatively weak inducer or, because in the absence of the methylamine transport system in non-induced cells, a sufficiently high intracellular level of methylamine is only slowly built up.
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  • 7
    ISSN: 1432-072X
    Keywords: Xanthobacter ; Methanol ; Formate ; Methylotrophy ; Autotrophy ; Calvin cycle ; Regulation ; Continuous culture ; RuBisC/O ; Carbon dioxide fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The regulation of C1-metabolism in Xanthobacter strain 25a was studied during growth of the organism on acetate, formate and methanol in chemostat cultures. No activity of methanol dehydrogenase (MDH), formate dehydrogenase (FDS) or ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisC/O) could be detected in cells grown on acetate alone over a range of dilution rates tested. Addition of methanol or formate to the feed resulted in the immediate induction of MDH and FDH and complete utilization (D=0.10 h-1) of acetate and the C 1-substrates. The activities of these enzymes rapidly dropped at the higher growth rates, which suggests that their synthesis is further controlled via repression by “heterotrophic” substrates such as acetate. Synthesis of RuBisC/O already occurred at low methanol concentrations in the feed, resulting in additive growth yields on acetate/methanol mixtures. The energy generated in the oxidation of formate initially allowed an increased assimilation of acetate (and a decreased dissimilation), resulting in enhanced growth yields on the mixture. RuBisC/O activity could only be detected at the higher formate/acetate ratios in the feed. The data suggest that synthesis of RuBisC/O and CO2 fixation via the Calvin cycle in Xanthobacter strain 25 a is controlled via a (de)repression mechanism, as is the case in other facultatively autotrophic bacteria. Autotrophic CO2 fixation only occurs under conditions with a diminished supply of “heterotrophic” carbon sources and a sufficiently high availability of suitable energy sources. The latter point is further supported by the clearly more pronounced derepressing effect exerted by methanol compared to formate.
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 171 (1953), S. 846-847 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Ponder3 concluded from his experiments that sodium taurocholate reacts with some definite component of the cells which is solubilized, but that the lysin is not bound to the walls. After equilibrium it was found that the sodium taurocholate concentration, which was estimated colorimetrically, had ...
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  • 9
    ISSN: 0147-619X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0147-619X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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