ISSN:
1573-4978
Keywords:
hepatitis B virus
;
inducible expression system
;
transcriptional activation
;
X protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006933629329
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