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  • 1
    Electronic Resource
    Electronic Resource
    Preparation of primary cell cultures from lamprey (1999)
    Springer
    Methods in cell science 21 (1999), S. 39-46 
    Details
    ISSN: 1573-0603
    Keywords: Ammocoete ; Fish cell cultrure ; Lamprey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lamprey is an important model for studies of evolution and comparative biology. The ability to culture cells from lamprey tissues makes it possible to employ an in vitro approach to address basic questions in these areas. Methods are described for the initiation of cell cultures derived from tissues of adult and larval sea lamprey (Petromyzon marinus). Primary cultures initiated from gill, muscle, gut, brain, ovary, heart and kidney were viable for up to eight months and several of the cultures were propagated for multiple passages. Most cultures were initiated from tissue explants in basal nutrient medium supplemented with fetal bovine and trout sera on a culture surface treated with fibronectin and collagen. Variations of these culture conditions to meet the specific growth requirements of certain cell types are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009824303498
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  • 2
    Electronic Resource
    Electronic Resource
    Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues (1992)
    Springer
    Cell biology and toxicology 8 (1992), S. 43-61 
    Details
    ISSN: 1573-6822
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: The zebrafish is a popular model for studies of vertebrate development and toxicology. However, in vitro approaches with this organism have not been fully exploited because cell culture systems have been unavailable. We developed methods for the culture of cells from blastula-stage diploid and haploid zebrafish embryos, as well as cells from the caudal and pelvic fin, gill, liver, and viscera of adult fish. The haploid embryo-derived cells differentiated in culture to a pigmented phenotype and expressed, upon exposure to 2,3,7,8-tetrachlorodibenzo p-dioxin, a protein that was immunologically and functionally similar to rainbow trout cytochrome P450IA1 Zebrafish cultures were grown in a complex basal nutrient medium supplemented with insulin, trout embryo extract, and low concentrations of trout and fetal bovine serum; they could not be maintained in conventional culture medium containing a high concentration of mammalian serum. Using calcium phosphate-mediated transfection, a plasmid constructed for use in mammalian cells was introduced into zebrafish embryo cell cultures and expressed in a stable manner. These results indicated that the transfection procedures utilized in mammalian systems can also be applied to zebrafish cell cultures, providing a means for in vitro alteration of the genotype and phenotype of the cells.[/ p]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00119294
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  • 3
    Electronic Resource
    Electronic Resource
    Cell cultures from zebrafish embryos and adult tissues (1994)
    Springer
    Methods in cell science 16 (1994), S. 99-107 
    Details
    ISSN: 1573-0603
    Keywords: Cell culture ; Fish embryo ; Zebrafish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The zebrafish is increasingly popular as a nonmammalian model for studies of vertebrate developmental biology, genetics, and toxicology. The availability of cell culture systems makes it possible to address many basic questions using in vitro approaches. Here we describe materials and procedures for initiating cell cultures from zebrafish early (blastula- and gastrula-stage) diploid and haploid embryos and adult tissues (gills, fins, liver, viscera). Zebrafish cells are grown in a complex basal nutrient medium supplemented with insulin, selenite, fetal bovine serum, trout serum, and an extract prepared from rainbow trout embryos. The procedure for preparing trout embryo extract (demonstrated to be mitogenic for a variety of piscine cell lines), is also described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404818
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  • 4
    Electronic Resource
    Electronic Resource
    Human and mouse S-protein mRNA detected in Northern blot experiments and evidence for the gene encoding S-protein in mammals by Southern blot analysis (1991)
    Springer
    Molecular and cellular biochemistry 100 (1991), S. 141-149 
    Details
    ISSN: 1573-4919
    Keywords: S-protein ; liver ; cell adhesion protein ; complement ; blood clotting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Human S-protein is a serum glycoprotein that binds and inhibits the activated complement complex, mediates coagulation through interaction with antithrombin III and plasminogen activator inhibitor I, and also functions as a cell adhesion protein through interactions with extracellular matrix and cell plasma membranes. A full length cDNA clone for human S-protein was isolated from a lambda gt11 cDNA library of mRNA from the HepG2 hepatocellular carcinoma cell line using mixed oligonucleotide sequences predicted from the amino-terminal amino acid sequence of human S-protein. The cDNA clone in lambda was subcloned into pUC18 for Southern and Northern blot experiments. Hybridization with radiolabeled human S-protein cDNA revealed a single copy gene encoding S-protein in human and mouse genomic DNA. In addition, the S-protein gene was detected in monkey, rat, dog, cow and rabbit genomic DNA. A 1.7 Kb mRNA for S-protein was detected in RNA from human liver and from the PLC/PRF5 human hepatoma cell line. No S-protein mRNA was detected in mRNA from human lung, placenta, or leukocytes or in total RNA from cultured human embryonal rhabdomyosarcoma (RD cell line) or cultured human fibroblasts from embryonic lung (IMR90 cell line) and neonatal foreskin. A 1.6 Kb mRNA for S-protein was detected in mRNA from mouse liver and brain. No S-protein mRNA was detected in mRNA from mouse skeletal muscle, kidney, heart or testis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234163
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  • 5
    Electronic Resource
    Electronic Resource
    Serum-free culture of carcinoma cell lines (1991)
    Springer
    Cytotechnology 5 (1991), S. 31-46 
    Details
    ISSN: 1573-0778
    Keywords: serum-free ; cell culture ; carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365532
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  • 6
    Electronic Resource
    Electronic Resource
    Cell cultures derived from early zebrafish embryos differentiate in vitro into neurons and astrocytes (1997)
    Springer
    Cytotechnology 23 (1997), S. 221-230 
    Details
    ISSN: 1573-0778
    Keywords: zebrafish ; neural differentiation ; fish cell culture ; fish embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The zebrafish is a polular nonmammalian model for studies of neural development. We have derived cell cultures, initiated from blastula-stage zebrafish embryos, that differentiate in vitro into neurons and astrocytes. Cultures were initiated in basal nutrient medium supplemented with bovine insulin, trout serum, trout embryo extract and fetal bovine serum. After two weeks in culture the cells exhibited extensive neurite outgrowth and possessed elevated levels of acetylcholinesterase enzyme activity. Ultrastructural analysis revealed that the neurites possessed microtubules, synaptic vessicles and areas exhibiting growth cone morphology. The cultures expressed proteins recognized by antibodies to the neuronal and astrocyte-specific markers, neurofilament and glial fibrillary acidic protein (GFAP). Poly-D-lysine substrate stimulated neurite outgrowth in the cultures and inhibited the growth of nonneuronal cells. Medium conditioned by the buffalo rat liver line, BRL, promoted the growth and survival of the cells in culture. Mitotically active cells were identified in cultures that had undergone extensive differentiation. The embryo cell cultures provide an in vitro system for investigations of biochemical parameters influencing zebrafish neuronal cell growth and differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007915618413
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  • 7
    Electronic Resource
    Electronic Resource
    First international symposium on marine molecular biology held at the Center of Marine Biotechnology, University of Maryland, Baltimore, Maryland on October 9–11, 1988 (1989)
    Springer
    Cytotechnology 2 (1989), S. 239-242 
    Details
    ISSN: 1573-0778
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00133249
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  • 8
    Electronic Resource
    Electronic Resource
    Culture of cells from tissues of adult and larval sea lamprey (1996)
    Springer
    Cytotechnology 21 (1996), S. 195-203 
    Details
    ISSN: 1573-0778
    Keywords: ammocoetes ; fish cell culture ; lamprey ; lampricide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Methods were developed for the culture of cells derived from tissues of the sea lamprey (Petromyzon marinus). Cultures were initiated from gill, liver, muscle and gut from larvae and newly transformed individuals and brain, heart, kidney and ovary from sexually mature adults. The lamprey cells were viable for up to six months in culture and cells from ovary, muscle, gut, gill and liver were propagated for multiple passages. For all cultures except liver, optimal cell attachment and spreading was obtained on surfaces coated with fibronectin and collagen. Optimal liver cell attachment was achieved on basement membrane. Cells synthesizing DNA were detected by precursor incorporation in five week-old cultures derived from adult and larval tissues. Metabolic labeling experiments with [35S]-methionine demonstrated that cultures initiated from liver and ovary continued to synthesize and release proteins into the medium for several weeks. Ultrastructural examination revealed the presence of ciliated cells in cultures from brain and the accumulation of lipid in epithelial cells derived from liver and gill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365342
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  • 9
    Electronic Resource
    Electronic Resource
    Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos (1994)
    Springer
    Cytotechnology 14 (1994), S. 21-26 
    Details
    ISSN: 1573-0778
    Keywords: fish cell culture ; fish embryo ; zebrafish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The zebrafish has become a popular model for studies of vertebrate development and toxicology. However,in vitro approaches utilizing this organism have not been fully exploited due to the absence of suitable cell culture systems. Previously, we developed methods for the culture of cells derived from zebrafish blastula-stage embryos. One of these cultures, ZEM-2, was derived in a complex medium containing trout embryo extract, trout serum and medium conditioned by buffalo rat liver cells. In this study we describe a zebrafish embryo cell line, ZEM-2A, derived from ZEM-2 following selection for growth in a simplified medium. Optimal growth of ZEM-2A cells is attained in nutrient medium supplemented with 5% fetal bovine serum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00772192
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  • 10
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    Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues (1992)
    Springer
    Details
    Publication Date: 1992-01-01
    Print ISSN: 0742-2091
    Electronic ISSN: 1573-6822
    Topics: Biology , Medicine
    Published by Springer
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