ISSN:
0935-6304
Keywords:
HPLC
;
Gradient elution
;
Cyano bonded phase
;
Polymerized vinyl alcohol bonded phase
;
Plant tissue
;
Potato
;
Lipid classes
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The wide range of lipid classes found in plant tissues, including simple lipids, glycolipids, and phospholipids, have been resolved by gradient high performance liquid chromatography with evaporative light-scattering detection. The lipids from potato tubers were used to optimize the separations. Adsorption chromatography with silica gel gave disappointing results for the plant glycolipids. Much better separations were obtained with chemically bonded stationary phases, e.g. those with propionitrile or polymeric vinyl alcohol as the functional moiety. With careful calibration, good reproducibility was possible. Only highly acidic lipids such as phosphatidylserine still present some problems.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240180205
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