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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 138 (1984), S. 187-190 
    ISSN: 1432-072X
    Keywords: Rhizobium ; Aromatic metabolism in protocatechuate 3,4-dioxygenase ; R. trifolii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protocatechuate 3,4-dioxygenase (EC 1.13.11.3) has been purified 42-fold from 4-hydroxybenzoate-grown cells of Rhizobium trifolii TA1, where it constitutes about 2% of the cell protein. The dioxygenase has a molecular weight of 220,000, with two dissimilar sub-units of molecular weights 29,000 and 26,500, corresponding to an α4β4 composition. The enzyme is specific for protocatechuate, with a Km of 1.75×10-5 M and maximum activity at pH 9.2. Metal removal and replacement studies indicate that the enzyme contains complexed Fe3+ which is required for activity. Direct atomic absorption analysis gave 1.3–1.5 g atoms Fe3+ per mole of isolated enzyme, but correction for metal-deficient proteins suggests that the value is close to 2.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 141 (1985), S. 225-228 
    ISSN: 1432-072X
    Keywords: Rhizobium trifolii ; Aromatic metabolism in catechol 1,2-dioxygenase ; R. trifolii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Catechol 1,2-dioxygenase has been purified 46-fold from cells of Rhizobium trifolii TA1 grown on benzoate plus glucose. The dioxygenase had a molecular weight of 107,000 and a sub-unit molecular weight of 59,000. The enzyme had a K m of 2 μM for catechol and also cleaved 4-methylcatechol. The dioxygenase contained 2 g atoms of Fe3+ per mole of enzyme which could be removed by treatment with 1,10-phenanthroline, resulting in a complete loss of activity; reactivation of the enzyme occurred specifically with Fe3+.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 151 (1989), S. 520-523 
    ISSN: 1432-072X
    Keywords: Rhizobium leguminosarum biovar trifolii ; Aromatic metabolism ; Mandelate ; 4-Hydroxymandelate ; Ketoadipate pathway
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhizobium leguminosarum biovar trifolii TA1 grows on 4-hydroxymandelate and enzymes involved in its catabolism are inducible. Strain TA1 does not grown on mandelate or cis, cis-muconate, but spontaneous mutants capable of growth on these substrates were isolated. Enzymes involved in mandelate degradation were also inducible. The presence of intermediates of the mandelate and hydroxymandelate pathways resulted in a significant decrease in some of the enzymes involved in their degradation. Succinate and acetate, end products of the pathways, and glucose caused reductions in the levels of enzymes in the mandelate and hydroxymandelate pathways.
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  • 4
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The heme-containing dehaloperoxidase from Amphitrite ornata was crystallized from an unbuffered solution containing 30% PEG 8000 and 200 mM ammonium sulfate by the hanging-drop vapor-diffusion method. Dark-red bipyramidal crystals are orthorhombic in space group P212121 with unit-cell dimensions a = 68.5, b = 68.4 and c = 61.1 Å. The asymmetric unit contains two subunits related by a non-crystallographic twofold axis. The crystals scatter beyond 2 Å resolution. The native data have been collected and one single-site mercury derivative has been found. SIRAS phasing was used to determine the positions of the heme Fe atoms and structure determination is in progress. A preliminary spectroscopic investigation indicates that the heme is protoporphyrin IX and its coordination sphere resembles that of a typical heme peroxidase, i.e. histidine ligated. Detailed spectroscopic and electrochemical studies are now under way.
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  • 5
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 78 (1995), S. 4031-4034 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Quantum, wires, ranging in width from 900 to 42 nm, were patterned onto a 10-nm-thick In0.2Ga0.8As quantum well in GaAs cladding then regrown by migration-enhanced epitaxy. Atomic-resolution transmission electron microscopy images of two of the quantum wires, one 400 nm wide and the other 42 nm wide, show lattice deformation of the quantum wires due to compression by the cladding. The lattice constant in the growth direction varies with horizontal position inside each wire, from largest in the wire center to smallest at the sidewalls. In the 400 nm wire, the lattice constant in the growth direction fully reaches the pseudomorphically strained value of 5.83 A(ring) at a distance of 165 A(ring) from the sidewall, while the lattice constant in the 42 nm wire reaches only 5.79 A(ring), at 75 A(ring) from the sidewall. From the value of the compressed lattice constant in the center of the 42 nm wire, the amount of strain in the center of the wire is inferred and, from this strain, the expected strain-induced band-gap energy shift is calculated. Photoluminescence measurements are made on the wires, showing a strain-induced increase in peak emission energy with decreasing wire size. That this energy shift is strain induced is verified by comparing it to the far smaller energy shift of an unregrown but, otherwise, identical sample, which has no regrowth-induced compressive strain. For the 42 nm quantum wire, after the calculated contribution due to increased quantum confinement is accounted for, the energy shift measured by photoluminescence is consistent with the calculated value to within the experimental error. © 1995 American Institute of Physics.
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  • 6
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 75 (1994), S. 3491-3499 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The suppression of longitudinally polarized optical-phonon (LOP) electron scattering in multiple quantum wells (MQWs) was sought in short periodic AlAs/GaAs with well widths of 12, 15, and 20 monolayers and AlAs barrier widths of 2 and 4 monolayers, based on a study of electron mobility in the plane of the MQW. Two-dimensional electron-gas structures with MQWs of up to eight wells in their channel were grown. Their mobilities at room temperature were slightly reduced, as compared to samples without MQW channel, due to interaction with interface polaritons from AlAs barriers, while mobility at temperatures 〈50 K improved due to reduction of remote ionized impurity scattering. The theoretical analysis of the results based on the model of hybridon-electron interaction in an infinite superlattice is presented. The reduction of room-temperature mobility in the MQWs is believed to be caused by the interaction of electrons with both barrier interface-polariton (IP) -like modes and the well LOP-IP hybrids. An alternative explanation of the results of a similar experiment done elsewhere is offered denying the evidence of strong suppression of LOP scattering there.
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  • 7
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 67 (1996), S. 2652-2653 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: An optimized current preamplifier, consisting of a shunt resistor followed by a high speed field-effect transistor input instrumentation amplifier, appears suitable for use in scanning tunneling microscopy. The preamplifier has a transimpedance of 100 MΩ, a measured 3 dB bandwidth of 200 kHz, low phase distortion, and excellent noise performance. © 1996 American Institute of Physics.
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  • 8
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 84 (1998), S. 4292-4299 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A comprehensive view of the microstructure of (111)B CdTe films grown on miscut (001)Si substrates by molecular beam epitaxy has been obtained by transmission electron microscopy and scanning transmission electron microscopy. It is found that in the initial growth stage, CdTe nucleates with a dominance of one particular domain: a domain with (111)B polarity and orientation of [11−2]CdTe//[1−10]Si, although there are also some other domains of different polarity and orientation. The dominance of one type domain is due to the reduction of the surface symmetry by using the miscut substrate and by using optimum growth conditions. As the growth proceeds, a single-crystal film is produced by the dominating domain overgrowing the minority domains nucleated at the film–substrate interface. This results in the final film of single-crystal character having (111)B polarity with [11−2]CdTe along [1−10]Si. © 1998 American Institute of Physics.
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  • 9
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Nonequilibrium electron distributions and phonons in CdTe have been studied by transient picosecond/subpicosecond Raman spectroscopy at T=300 K. Our experimental results show that for photoexcited electron–hole density of n(approximately-equal-to)1018 cm−3, the electron distributions can be reasonably well described by Fermi–Dirac distribution functions with effective electron temperature substantially higher than the lattice temperature. From an ensemble Monte Carlo analysis of the nonequilibrium phonon population as a function of photoexcited electron–hole pair density, the LO phonon lifetime in CdTe has been deduced to be τ(approximately-equal-to)0.75±0.25 ps. © 1996 American Institute of Physics.
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  • 10
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 71 (1997), S. 1810-1812 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We describe the growth of high quality CdTe(211)B layers by molecular beam epitaxy on nominal Si(211) substrates. Prior to CdTe deposition, thin ZnTe(211)B buffer layers were grown to preserve the homo-orientation. Large-area CdTe(211)B layers were routinely obtained by optimizing the growth parameters. From x-ray diffraction, we observed the presence of twin-free CdTe(211)B layers. One 8 μm thick CdTe epilayer had a near-surface etch pit density of 1.5×105 cm−2, which surpassed the best value reported for CdTe(211)B grown on GaAs(211)B, GaAs/Si(211), or Si(211) substrates. © 1997 American Institute of Physics.
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