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  • 1
    Electronic Resource
    Electronic Resource
    Somatic hybridization of sexually incompatible petunias: Petunia parodii, Petunia parviflora (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 1-4 
    Details
    ISSN: 1432-2242
    Keywords: Petunia parodii ; Petunia parviflora ; Sexual Incompatibility ; Somatic hybridisation ; Horticulture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrid plants were regenerated following the fusion of leaf mesophyll protoplasts of P. parodii with those isolated from a nuclear-albino mutant of P. parviflora. Attempts at sexual hybridization of these two species repeatedly failed thus confirming their previously established cross-incompatibility. Selection of somatic hybrid plants was possible since protoplasts of P. parodii would not develop beyond the cell colony stage, whilst those of the somatic hybrid and albino P. parviflora produced calluses. Green somatic hybrid calluses were visible against a background of albino cells/calluses, and upon transfer to regeneration media gave rise to shoots. Shoots and the resultant flowering plants were confirmed as somatic hybrids based on their growth habit, floral pigmentation and morphology, leaf hair structure, chromosome number and Fraction 1 protein profiles. The relevance of such hybrid material for the development of new, and extensively modified cultivars, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276001
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  • 2
    Electronic Resource
    Electronic Resource
    Somatic hybrids between unilateral cross-incompatible Petunia species (1979)
    Springer
    Theoretical and applied genetics 55 (1979), S. 97-99 
    Details
    ISSN: 1432-2242
    Keywords: Petunia ; Unilateral cross-incompatibility ; Protoplast fusion ; Somatic hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrid plants regenerated following the fusion of leaf mesophyll protoplasts of Petunia parodii with those isolated from a cell suspension of albino P. inflata. These two species exhibit a unilateral cross-incompatability with a pre-zygotic mode of reproductive isolation preventing hybridizations with P. inflata as the maternal parent. Selection of somatic hybrids relied on the fact that unfused or homokaryon protoplasts of P. parodii did not develop beyond the cell colony stage while those of the putative somatic hybrids and albino P. inflata parent produced callus. Green somatic hybrid calluses were readily identified against the white background of P. inflata following complementation to chlorophyll synthesis proficiency and continued growth in hybrid cells. Shoots, and ultimately flowering plants, were identified as somatic hybrids based on their floral morphology and colour, chromosome number and the fact that they segregated for parental characters. The frequency of somatic hybrid production was comparable to that previously established for two sexually compatible Petunia species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295433
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    Paper (German National Licenses)
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