ISSN:
1573-5087
Keywords:
phytochrome
;
infrared
;
video
;
Pisum sativum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract The kinetics of phototropism were determined from infrared video recordings of third internodes of intact 7 d etiolated pea seedlings (Pisum sativum L. cv Alcan) exposed to continuous, unilateral blue light (460 nm). Small but significant curvatures (ca. 1–2°) were observed after a 2 h stimulation at 0.8 pmol m-2 s-1. Both latency (i.e., lag time) and rate of curvature were dependent on irradiance between 0.8 and 80 pmol m-2 s-1. Maximum rates of curvature at the highest irradiance of blue light were 74% greater in seedlings that received 2 min red light (270 J m-2) 20 h earlier, but latency was unaffected. An irradiance-response curve for curvature at 2 h was obtained with unilateral, broad-spectrum blue light between 4 and 90,000 pmol m-2 s-1. Maximum tropistic response in dark-grown seedlings (ca. 25°) occurred between 30 and 1000 pmol m-2 s-1. Peak response for red light-treated seedlings (ca. 55°) was obtained at 1000 pmol m-2 s-1. Above this irradiance, phototropism declined by 12–15° for both red-treated and dark-grown plants. Red light did not significantly stimulate phototropism at lower, limiting irradiances of blue light. The data do not support the hypothesis that phytochrome affects the amount or activity of a photoreceptor for phototropism.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00027209
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