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  • 1
    Electronic Resource
    Electronic Resource
    Expression of resistance to Mycosphaerella pinodes in Pisum sativum (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 41 (1992), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The infection of three resistant and two susceptible inbred lines of Pisum sativum by Mycosphaerella pinodes is described for the first time. Two types of resistance, one expressed in epicotyls and one in leaves, were found in all three resistant lines. On epicotyls of susceptible lines, abundant appressoria and penetrations occurred after a short period of hyphal growth. On epicotyls of resistant lines, hyphae grew extensively but rarely formed appressoria, and these failed to penetrate the cuticle. Attempted penetration was associated with the rapid death of 2–6 epicotyl cells, resembling a hypersensitive reaction. In contrast, resistance of leaves, which was only expressed after penetration, involved localization of the fungus by a mechanism involving delayed leaf cell death. It is suggested that a combination of these two types of resistance might provide effective protection against M. pinodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1992.tb02359.x
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  • 2
    Electronic Resource
    Electronic Resource
    Widespread introgression of Solatium phureja DNA in potato (S. tuberosum) dihaploids (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 116 (1997), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Dihaploids were assumed to be of parthenogenic origin but cv. ‘Pentland Crown’ dihaploids produced at the Scottish Crop Research Institute (SCRI), Dundee, Scotland, are known to contain DNA from the S. phureja dihaploid inducer. This study investigates whether inducer DNA occurs in dihaploids of cultivars ‘Aminca’, ‘Brio’, ‘Lizen’ and ‘Sirtema’, produced at INRA, Ploudaniel, France. Two microsatellite markers and five simple sequence repeat 5′-anchored polymerase chain reaction (PCR) primers were used to generate markers. Markers originating from the dihaploid inducer were detected in 13 of the 19 INRA dihaploids and in some dihaploids from each cultivar. Greater introgression occurred in ‘Lizen’ dihaploids than in ‘Brio’ dihaploids, suggesting that the female (S. tuberosum) parent influences introgression. The percentage of INRA dihaploids containing inducer DNA was similar to that for ‘Pentland Crown’ dihaploids (c. 65%). The micro-satellite markers provided the first evidence of the transfer of specific, potentially useful, genes from the inducer to the dihaploid offspring.Interspecific introgression during dihaploid induction is more widespread than previously thought and researchers should be aware that it may influence the results of research using dihaploids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1997.tb01010.x
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  • 3
    Electronic Resource
    Electronic Resource
    Evidence that genes from the male parent may influence the morphology of potato dihaploids (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 241-248 
    Details
    ISSN: 1432-2242
    Keywords: Key words Potato dihaploids ; Principal component analysis ; Dihaploid induction ; Microsatellite ; Isozyme analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A number of recent studies have provided evidence that potato dihaploids (S. tuberosum) contain and express DNA from the male (dihaploid inducer) parent, S. phureja. The importance of this for breeding programmes that use dihaploid potatoes is to some extent dependent upon whether the S. phureja DNA influences dihaploid morphology. In the present study, 21 characters were used to compare the morphology of six dihaploids with those of their parents: S. tuberosum (cvs `Pentland Dell' and `Pentland Crown') and S. phureja (IVP48). Characteristics of S. phureja were found in all of the dihaploids examined. In principal component analyses, dihaploids formed intermediate groupings positioned between those of the parents, although much closer to S. tuberosum. It is concluded there is evidence that DNA originating from the dihaploid inducer can affect the morphology of potato dihaploids. Implications of the findings are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050406
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  • 4
    Electronic Resource
    Electronic Resource
    Cytological and molecular observations on Solanum phureja-induced dihaploid potatoes (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 545-551 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; Dihaploid ; Parthenogenesis ; Fertilization ; Chromosome elimination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seventeen potato dihaploids, produced by pollinating the tetraploid (2n = 48) cv ‘Pentland Crown’ with pollen from Solanum phureja (2n = 24) dihaploid inducer clones, were studied. Since dihaploids are thought to develop parthenogenetically from unfertilized ovules they were expected to be euploid (2n = 24), but somatic chromosome counts showed that 15 of the 17 dihaploids were aneusomatic. Ten of the clones were predominantly diploid (2n = 24) with a proportion of hyperploid cells that contained 25 or 26 chromosomes. Five of the dihaploids contained variable numbers of triploid cells (2n = 36). RFLP analysis was used to determine whether the additional chromosomes were from S. phureja or S. tuberosum. Unique hybridizing fragments present in S. phureja but not in ‘Pentland Crown’ were identified. These S. phureja-specific restriction fragments were present in some of the dihaploid offspring of ‘Pentland Crown’. Of the 5 clones that contained triploid cells 4 had S. phureja type banding. Four of the 10 aneusomatic clones that contained hyperploid cells had the unique S. phureja hybridizing fragments. We propose that ovules of ‘Pentland Crown’ were fertilized by pollen from S. phureja and that the aneusomatic clones were derived from triploid zygotes from which some of the S. phureja chromosomes were eliminated. We consider that this is an additional mechanism of dihaploid formation in potato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226789
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  • 5
    Electronic Resource
    Electronic Resource
    Producing commercially attractive, uniform true potato seed progenies: the influence of breeding scheme and parental genotype (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 519-525 
    Details
    ISSN: 1432-2242
    Keywords: True potato seed ; Commerical attractiveness ; Uniformity ; General combining ability ; Desynapsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The production of attractive, uniform true potato seed (TPS) progenies was investigated. Four breeding schemes were compared: intercrossing tetraploid cultivars (cv x cv); doubled dihaploids x cultivars (ddh x cv); cultivars x diploid unreduced-gamete producers (cv x FDR) and doubled dihaploids x diploid unreduced-gamete producers (ddh x FDR). Fifty three progenies and five clones were grown in a glasshouse in a randomised complete block design with three replicates of 25 plants per progeny and clone. Each plant's tubers were counted and the colour, shape, quality of skin finish, flesh colour, and commencai attractiveness (which includes yield) recorded. The most uniform progenies were also selected by visual comparison with the clones. For mean attractiveness, differences (P 〈 0.001) between breeding schemes and between progenies within breeding schemes were detected. The cv x cv and cv x FDR progenies were more attractive than clonal controls. There were significant additive and non-additive effects for attractiveness in all breeding schemes except cv x FDR. There were between-progeny differences (P 〈 0.001) for uniformity for all characters. Progenies uniform for one character could be variable for other traits. Breeding schemes gave different levels of uniformity (P 〈 0.001) for all characters except shape and flesh colour, but none gave low levels of variation for all traits. Doubled-dihaploid parents increased the variation in progenies. There were uniformity differences (P 〈 0.001) between progenies within breeding schemes for all characters. Evidence of additive and nonadditive genetic variation for uniformity in all traits was detected. In each breeding scheme, parents with good general combining ability (GCA) for uniformity in several characters were identified. Visually selected uniform progenies had parents with good GCAs for uniformity in a range of traits and high specific combining abilities (SCAs) for several traits. A desynaptic first-division restitution (FDR) clone and a male-sterile doubled-dihaploid clone had the best GCAs for tuber uniformity in TPS progenies. Achieving multitrait uniformity in TPS is problematic but may be aided by the selection of parents with GCAs for uniformity coupled with progeny testing to allow for non-additive effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221998
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  • 6
    Electronic Resource
    Electronic Resource
    Solanum phureja genes are expressed in the leaves and tubers of aneusomatic potato dihaploids (1993)
    Springer
    Euphytica 69 (1993), S. 1-6 
    Details
    ISSN: 1573-5060
    Keywords: gene expression ; isozymes ; patatin ; potato dihaploids ; Solanum phureja
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The expression of leaf isozymes and tuber patatin in dihaploids derived from the Solanum tuberosum cv. Pentland Crown was investigated. Seven of the dihaploids were aneusomatic containing additional chromosomes from the S. phureja dihaploid inducer. Of these, four genotypes expressed leaf isozymes characteristic of the S. phureja dihaploid inducer, and the tubers of three aneusomatic dihaploids contained a S. phureja form of patatin. Aneusomatic dihaploids in which the proportion of cells containing additional S. phureja chromosomes was relatively small (i.e. 1–15%) did not express leaf isozyme markers or patatin bands characteristic of the dihaploid inducer or showed only faint expression of one or two markers. However, those with a high proportion of cells containing additional chromosomes (50–55%) had a range of strongly expressed leaf isozymes that were characteristic of the dihaploid inducer and also expressed the S. phureja tuber patatin. One dihaploid genotype was exclusively euploid (2n〈24), yet is expressed a S. phureja leaf isozyme marker and S. phureja tuber patatin, suggesting recombination or chromosome substitution between the genome of the S. phureja dihaploid inducer and the cultivar Pentland Crown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021720
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  • 7
    Electronic Resource
    Electronic Resource
    Genetical analysis of resistance to Mycosphaerella pinodes in pea seedlings (1991)
    Springer
    Euphytica 58 (1991), S. 183-189 
    Details
    ISSN: 1573-5060
    Keywords: Mycosphaerella pinodes ; pea ; Pisum sativum ; genetics ; disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In studies of the inheritance of resistance, pea seedlings of seven lines in which stems and leaves were both resistant to Mycosphaerella pinodes were crossed with a line in which they were both susceptible. With seven of the crosses resistance was dominant to susceptibility. When F2 progenies of five crosses were inoculated on either stems or leaves independently, phenotypes segregated in a ratio of 3 resistant: 1 susceptible indicating that a single dominant gene controlled resistance. F2 progenies of one other cross gave ratios with a better fit to 9 resistant: 7 susceptible indicating that two co-dominant genes controlled resistance. The F2 progeny of another cross segregated in complex ratios indicating multigene resistance. When resistant lines JI 97 and JI 1089 were crossed with a susceptible line and leaves and stems of each F2 plant were inoculated, resistance phenotypes segregated independently demonstrating that leaf and stem resistance were controlled by different genes. In two experiments where the F2 progeny of the cross JI 97×JI 1089 were tested for stem and leaf resistance separately, both characters segregated in a ratio of 15 resistant:1 susceptible indicating that these two resistant lines contain two non-allelic genes for stem resistance (designated Rmp1 and Rmp2) and two for leaf resistance (designated Rmp3 and Rmp4). Evidence that the gene for leaf resistance in JI 1089 is located in linkage group 4 of Pisum sativum is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022819
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  • 8
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    Amphibians on the brink (2017)
    American Association for the Advancement of Science (AAAS)
    In: Science
    Details
    Publication Date: 2017-08-04
    Keywords: Ecology, Epidemiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Geosciences , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 9
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    Evidence for somatic translocation during potato dihaploid induction (1995)
    Springer Nature
    Details
    Publication Date: 1995-02-01
    Print ISSN: 0018-067X
    Electronic ISSN: 1365-2540
    Topics: Biology
    Published by Springer Nature
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  • 10
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    Impacts of invasive cane toads on an endangered marsupial predator and its prey (2021)
    Inter-Research
    Details
    Publication Date: 2021-01-01
    Print ISSN: 1863-5407
    Electronic ISSN: 1613-4796
    Topics: Biology
    Published by Inter-Research
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