ISSN:
1040-0397
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The on-line high-performance liquid chromatography-electrochemistry-thermospray mass spectrometry (LCEC-LC/MS) for separation and identification of the phenolic isoflavones genistein, formononetin, and Biochanin A is described. The isoflavones are separated on a C-18 reversed phase column with methanol: 0.1 M ammonium acetate pH (70:30 v/v) as eluent, which is compatible for both LCEC and LC/MS detection. Low ng detection is achieved with a conventional glassy carbon thin-layer electrochemical cell at 1.3 V vs. Ag/AgCl. Selected ion monitoring (SIM) mode LCMS results in detection levels at the 20-50 ng range, and the compounds are positively identified. In the on-line detection, the electrochemical cell is connected to the thermospray and the column effluent flows from the working electrode into the heated thermospray capillary tube. The results show that on-line coupling between a thin-layer LCEC and a thermospray-LC/MS can be used to characterize analytical systems.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140030810
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