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  • 1
    ISSN: 1432-0983
    Keywords: Episomal plasmid ; Chromosomal integration ; Plasmid rescue ; Gene seduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Yeast diploids containing a chromosomally integrated episomal plasmid manifest the mitotic instability of the chromosomes with the plasmid. Probably as a results of the destabilization integrated plasmid may be excised out of the chromosome. It was found that the rescue of the plasmid may be irregular, with the taking up of adjacent chromosomal gene. Using an integrant with the plasmid integrated into chromosome I very near the ADE1 locus we cloned this gene “in vivo” by selecting rescued plasmid marker LEU2. The new plasmid has retained the LEU2 gene and the capacity to replicate autonomously. This plasmid was used to transfer the cloned ADEI gene from one cell to another by transformation and from one resident chromosome to another by integration. The phenomenon of irregular excision of an integrated episomal plasmid together with linked chromosomal gene(s) and transfer to other chromosomes or cells we propose to term Seduction.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: Yeast ; 2 μm plasmid ; Mitotic recombination ; Coincident conversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of the 2 μm plasmid on homologous recombination in the right arm of chromosome XV of the yeast Saccharomyces cerevisiae has been examined. No differences between spontaneous mitotic recombination rates in [cir 0] and [cir +] derivatives of two yeast diploid tester strains were detected. In the course of analysis an unusually high coincident conversion frequency at ADE2, HIS3, and two RFLP loci adjacent to ADE2, was observed. The character of coincident homozygotization of linked markers argues for a “break-and-replicate” mechanism underlying the coincident conversion events.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Metal science and heat treatment 17 (1975), S. 712-714 
    ISSN: 1573-8973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Conclusions Microalloying of Cr−Ni−Mn steel with boron in amounts of 0.002–0.005% (nominal) increased the ductility at 900–1100° by 100–125%, making it insusceptible to hot shortness. This improves the deformability of the ingots and slabs. With an increase of the boron concentration from 0.005 to 0.10% the ductility of the steel is greatly impaired at temperatures above 1100°. In view of the susceptibility of the steel to overheating at temperatures above 1260°, the optimal temperature for heating the ingots is 1240–1260° on condition that they be held in the range of 1200–1220° in the first stage of heating.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Metal science and heat treatment 19 (1977), S. 398-400 
    ISSN: 1573-8973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Conclusions 1. As the solidus temperature is approached the fracture of high-carbon carbide steels occurs due to the solution of carbides, saturation of the solid solution with carbon, and the formation of carcks in sections rich in carbon. Internal oxidation of the boundaries was not observed. 2. Overheating of alloy steels with 1.2–2% C above the temperature at which the boundaries melt can be corrected by cooling the overheated ingots 10–50° below the maximum permissible temperature. After holding at this temperature, the structure is more suitable for deformation, and the plasticity is restored and even improved.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Metal science and heat treatment 12 (1970), S. 968-969 
    ISSN: 1573-8973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Metal science and heat treatment 13 (1971), S. 1047-1049 
    ISSN: 1573-8973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Conclusions 1. The ductility of Fe−Cr alloys containing 40–50% Cr can be increased by alloying with up to 3.5–4% Al and suitable hot deformation conditions. 2. The pressure treatment for cast alloys tested in this work is optimal for alloys with up to 40% Cr and 3% Al. For cast and wrought alloys of this system with a larger amount of chromium and aluminum the optimal processes for obtaining high-quality semifinished products are characterized by low rates and high values of the isotropic stress tensor and different types of extrusion. 3. Superplasticity in alloys with 40–45% Cr and 1.7–4% Al can be achieved by a deformation rate of (1–2)·10−2 sec−1 at 1050–1150°C.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Metal science and heat treatment 11 (1969), S. 552-555 
    ISSN: 1573-8973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Conclusions 1. The wear resistance of steels for soil-cutting tools can be increased 30–50% by increasing the carbon content from 1.5 to 2%, the chromium content from 6 to 12%, and the addition of 1.5–2.0% Ti and V. 2. It was found that carbon reduces the technological ductility the most (at 2.50% C the limit heating temperature is 1150°C). 3. Vanadium, titanium, manganese, and silicon within limits of 1.5–2.0% slightly reduce the plasticity of Kh6F1 steel, while an increase of the chromium concentration to 12% reduces the harmful influence of carbon on the plasticity.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Metal science and heat treatment 15 (1973), S. 405-407 
    ISSN: 1573-8973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Conclusions 1. The temperature dependence of the ductility of steels of the Kh6F(T) type (maximum ductility at temperatures considerably below TS) can be explained by the joint influence of diffusion and acting stresses. 2. Wear resistant steels with 1.5–2% C, 6–12% Cr, and 1–3% V or 1–2% Ti are recommended for operation at temperatures up to 400°C. 3. Raising the vanadium or titanium content to 2–3% has no substantial effect on the strength or ductility of steels with 1.5% C and 6% Cr.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Metal science and heat treatment 21 (1979), S. 420-423 
    ISSN: 1573-8973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Conclusions 1. Ternary Fe-Mn-C alloys containing 12–45% Mn and 1.2–2.5% C exhibit structural superplasticity after deformation in tension at 770–950°C at strain rates of (0.2–2)·10−3 sec−1 both in air and in vacuum. 2. Plastic deformation of samples with an original uneven structure is accompanied by refining and homogenization of the structure due to recrystallization of the alloy and diffusion of carbon, but the plasticity is far lower in this case.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Metal science and heat treatment 8 (1966), S. 455-460 
    ISSN: 1573-8973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Conclusions 1. During hot rolling with 20–30% swaging at 750–950°C zones of small grains are formed at the surface of sheets of steel containing 3% Si. The thickness of this zone increases with increasing degrees of deformation. The grain size in the surface zone is the main factor determining the plasticity of such steel during subsequent cold deformation. 2. In a large grain steel the main factor affecting plasticity is the amount of the precipitates of the second phase along the grain boundaries. A large amount of precipitates leads to premature twinning, which, at low temperatures, favors the formation of microcracks.
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