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  • 1
    Electronic Resource
    Electronic Resource
    Mobilisation of storage reserves during germination and early seedling growth of sugar beet (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 78 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The principal storage reserve of sugar beet seeds is starch, which is localised in the perisperm. Additional storage reserves include the seed proteins, albumins, globulins and glutclins, which are exclusively located in the embryo. Soluble sugars are also detectable in all the organs of the mature seed. The time-course of reserve mobilisation in the different organs of the sugar beet (Beta vulgaris L. cv. Regina) seed during germination and early seedling growth is documented, with particular reference to changes in (a) activities of hydrolases: a-amylase, β-amylase, and α-glucosidases; (b) levels of carbohydrates and (c) proteins. Amylase activities increase substantially in both cotyledons, as well as the perisperm, whereas the increase in α-glucosidase activities is largely confined to the perisperm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb09058.x
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  • 2
    Electronic Resource
    Electronic Resource
    Defense-Related Proteins in Higher Plants (1990)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Biochemistry 59 (1990), S. 873-907 
    Details
    ISSN: 0066-4154
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Chemistry and Pharmacology , Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.bi.59.070190.004301
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  • 3
    Electronic Resource
    Electronic Resource
    The role of pH and ion transport in oligosaccharide-induced proteinase inhibitor accumulation in tomato plants (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 13 (1990), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. The induction of proteinase inhibitor (PrI) activity in young tomato plants by wounding and oligosaccharides has been shown to be prevented by pretreatment of the plants with phenolic acids such as aspirin. Aspirin applied over a wide range of concentrations is able to inhibit PrI induction by pectic fragments. The possibility that other weak acids may also inhibit PrI induction was investigated. Isobutyric acid and trimethylacetic acid were shown to be less effective as inhibitors than aspirin, and weak bases were without effect. However, it was demonstrated that various agents known to influence ATPase activity and intracellular pH were able to inhibit PrI induction, and in particular the striking effect of low concentrations of fusicoccin on PrI induction was noted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1990.tb01103.x
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  • 4
    Electronic Resource
    Electronic Resource
    Oligosaccharides that induce proteinase inhibitor activity in tomato plants cause depolarization of tomato leaf cells (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 13 (1990), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Two size ranges of oligosaccharide elicitors of pectic origin have been investigated for their effects on tomato plants. Both size ranges, with degrees of polymerization of 1–7 and 10–20 respectively, induced the accumulation of proteinase inhibitor (PI) activity in excised plants, and also induced changes in membrane potential of leaf mesophyll cells. The depolarizations were substantial, rapid, and reversible on removal of the elicitors. The effects are discussed in the context of early events in the signal transduction pathway linking oligosaccharides to changes in PI gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1990.tb01074.x
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  • 5
    Electronic Resource
    Electronic Resource
    Changes in water relations and endogenous abscisic acid content of wheat and barley grains and embryos during development (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 14 (1991), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Immature cereal embryo development can be controlled by in vitro culture on media containing ABA, or by media of low osmotic potential. To assess the possible in vivo roles of these factors, endogenous ABA levels and water relations of embryos and grains of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) were determined during development. ABA concentrations remained consistent with those required to inhibit precocious germination in vitro of early stage embryos but not of more mature embryos. With increasing maturity, a difference in water potential developed between grain and embryo, suggestive of an in vivo role for water status in controlling the development of the embryo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1991.tb00955.x
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  • 6
    Electronic Resource
    Electronic Resource
    Electrical signalling and systemic proteinase inhibitor induction in the wounded plant (1992)
    [s.l.] : Nature Publishing Group
    Nature 360 (1992), S. 62-65 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Mechanical damage applied to the lamina of one of the cotyledons of a tomato seedling with one expanded leaf (Fig. \a) leads to electrical activity (Fig. \b) which can be detected by extracellular electrodes placed on the stem and on the petiole of leaf 1, morphologically the lowest (first-formed) ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/360062a0
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of endogenous abscisic acid levels in immature cereal embryos during in vitro culture (1988)
    Springer
    Planta 173 (1988), S. 110-116 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid in embryos ; Embryo development ; Hordeum (abscisic acid) ; Lectin ; Osmotic stress ; Triticum (abscisic acid)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Levels of endogenous abscisic acid (ABA) in immature wheat (Triticum aestivum cv. Timmo) and barley (Hordeum vulgare cv. Golden Promise) embryos have been determined by enzyme-linked immunosorbent assay. Embryos of both cereal species showed an increase in ABA content during development on the parent plant. Immature embryos were excised and cultured in vitro on nutrient media that led to precocious germination or on media containing 9% (w/v) mannitol that maintained their developmental arrest. Barley and wheat embryos responded to these culture conditions in an identical manner with respect to changes in morphology, fresh weight, protein and lectin content. However, in complete contrast, the ABA content of barley embryos increased by an order of magnitude during culture on mannitol, whereas that of wheat embryos showed no significant change. The results are discussed within the context of the role of ABA in the regulation of embryo development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394495
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  • 8
    Electronic Resource
    Electronic Resource
    Appearance of wheat-germ agglutinin during maturation of field-grown wheat (1985)
    Springer
    Planta 165 (1985), S. 377-382 
    Details
    ISSN: 1432-2048
    Keywords: Endopeptidase ; Lectin ; Triticum (lectin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Field-grown wheat (Triticum aestivum L.) has been used as a developmental system to study the appearance of wheat-germ agglutinin during grain maturation. The lectin appears at the mid-grain growth period (30–34 days post-anthesis) and continues to be synthesised throughout the late stages of maturation and desiccation. An acidic endopeptidase activity, inhibited by pepstatin-phenanthroline is present in extracts of embryo and endosperm throughout maturation. After in-vivo labelling of immature embryos with [35S]methionine for 3 h and extraction in the presence of proteinase inhibitors, immunoprecipitates with anti-wheat-germ agglutinin were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography, and found to contain three 35S-labelled polypeptides of Mr 46000, 18000 and 13000. Comparison of two-dimensional tryptic maps of 125I-labelled peptides indicate the three polypeptides are closely related.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392235
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  • 9
    Electronic Resource
    Electronic Resource
    Changes in the levels of wheat- and barley-germ agglutinin during embryogenesis in vivo, in vitro and during germination (1985)
    Springer
    Planta 166 (1985), S. 407-413 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid and precocious germination ; Embryogenesis ; Hordeum (lectin) ; Lectin ; Triticum (lectin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Radioimmunoassay has been used to measure levels of wheat-germ agglutinin and barley-germ agglutinin during embryogenesis and germination. The two lectins exhibited similar patterns of accumulation during grain maturation in vivo and both decreased to low levels after imbibition of harvest-ripe grains for 3 d. Precocious germination of immature wheat and barley embryos excised and cultured in vitro could be prevented either by inclusion of abscisic acid or mannitol in the culture medium. Changes in the level of wheat-germ agglutinin induced by in vitro culture depended on the maturation stage of the embryo. No direct correlation was found between application of exogenous abscisic acid and accumulation of the lectin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401180
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  • 10
    Electronic Resource
    Electronic Resource
    Lectin levels in tissues of cultured immature wheat embryos (1986)
    Springer
    Plant cell reports 5 (1986), S. 460-463 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Levels of wheat germ agglutinin have been determined by radioimmunoassay in tissues of immature wheat embryos cultured under different conditions in order to determine the suitability of the lectin as a marker for somatic embryogenesis. Embryos cultured on media favouring continued embryo development accumulated lectin in a similar manner to zygotic embryos in planta unless precocious germination occurred. Embryos cultured on media containing 2,4-D produced callus, and some of this developed somatic embryos. Both embryogenic and non-embryogenic callus contained WGA, that in non-embryogenic callus possibly arising from developmentally arrested root primordia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269642
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