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  • 1
    Monograph available for loan
    Monograph available for loan
    Mainz : Landesamt für Wasserwirtschaft Rheinland-Pfalz
    Associated volumes
    Call number: M 99.0180
    In: Sanierungskonzept Lahn, Rheinland-Pfalz
    Type of Medium: Monograph available for loan
    Pages: 178 S.
    ISBN: 393312302X
    Classification:
    Applied Geology
    Location: Upper compact magazine
    Branch Library: GFZ Library
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 8 (1969), S. 4606-4612 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1424
    Keywords: Erythrocyte ; Cytoskeleton ; Cell deformability ; Anion transport inhibitors ; Band 3 ; Sickle cell disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two nonpenetrating membrane probes, p-azidobenzylphlorizin (p-AzBPhz) and 4,4′-diisothiocyano-2,2′-stilbene disulfonate (DIDS), have been shown in earlier studies to induce dose-dependent changes in red blood cell (RBC) shape and volume at the same low concentrations that inhibit anion transport. In the present work, these ligand-induced morphology and rheology changes were studied using video digital image morphometry (VDIM) and microfiltration techniques. The results of these experiments corroborate our earlier investigation. RBCs were filmed using a Nomarski optics microscope with video camera attachment and cell size and shape changes were computer analyzed using VDIM. Low μm p-AzBPhz or DIDS levels caused collapse of the cell's biconcave structure and cell flattening occurred within 1–2 sec after drug exposure. Higher doses of either agent converted cells to a new steady-state in which a concurrent limited increase in erythrocyte volume and blunt membrane protrusions were produced. These changes were reversed in less than 2 sec by washing the drug from the membrane. Both ligands increased the deformability of RBCs in a dose-dependent manner as determined by filtration through Nuclepore polycarbonate filters (3 μm pore diameter). The improvement in deformability of drug-treated sickle cells was much more dramatic than for normal cells at low p-AzBPhz concentrations. These results support our earlier conclusions that the ligands, through a common interaction with band 3, induce volume-associated cytoskeletal alterations which lead to changes in morphology and flexibility.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 18 (1974), S. 351-364 
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary In dilute suspensions of sperm cells there is electrode noise due to the changes in the electrical double layer during contacts of the suspended cells with the electrode surface. The noise frequency, the number of current fluctuations above one nanoampere per unit time, depends upon the concentration of cells, the potential at the electrode surface and the presence of adsorbed material at the electrode. We have studied the electrode noise due to suspensions of rabbit sperm cells, and have obtained information about the properties of the cell membranes and their ability to adsorb or interact with substances in the environment. The observed differences in the noise frequency between ejaculated and epididymal sperm cells indicate that the ejaculated sperm cell does not adsorb material as readily. The titration of sperm cell suspensions with Zn++ ions shows a lower negative surface charge on the epididymal sperm cell, in agreement with published values on cell electrophoresis. The experiments reported here demonstrate that there are significant changes in the membranes of sperm cells at the later stages of maturation which are independent of membrane charge and affect the ability of the cells to adsorb material. Such adsorption processes could be the first stages of physiological processes (e.g. “sperm capacitation”, “acrosome reaction”) that are known to occurin vivo, and that play important roles in fertilization.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 29 (1976), S. 401-409 
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary When mammalian sperm cells are exposed to solutions of albumin there are changes in the membranes of some species that resemble those that normally occur in the uterus prior to fertilization. We have shown that albumin molecules adsorb on to the membranes of ejaculated rabbit sperm cells, and that the equilibrium binding constant,K, (1) varies inversely with the albumin concentration, (2) is independent of the sperm cell concentration in the range 106–107 per ml, (3) is independent of the time of exposure of the sperm cells to the albumin solution, and (4) decreases in the presence of Ca++ and Mg++ ions. An unusual aspect of the adsorption is that if the albumin concentration is given the symbol [A],K[A] is a constant in our measurements. This means that for virtually the entire range of [A] studied, the sperm cells bind albumin so that half of the available surface is coated and half remains uncoated. This situation is rather remarkable and it suggests a role that adsorption could play in the physical processes preceding fertilization. In purely physical systems, the optimum for the bridging and flocculation of particles that are coated with adsorbed macromolecular films occurs when half of the available surface is covered. The sperm cell appears to provide the optimal situation for interacting with itself or with another surface.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 27 (1962), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Studies were carried out on the fractionation and analysis of egg lipid by several new techniques employing thin-layer chromatography. The fatty acid composition of the major lipid classes and subfractions thereof was determined by a combination of alkali-isomerization and gas-liquid chromatography. The positional arrangements of the fatty acids in the glycerides and lecithin fraction were determined. The fatty acids in lecithin were distributed mainly in one type, the α-saturated β-unsaturated type, in contrast to the fatty acids of triglycerides.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 985 (1989), S. 67-74 
    ISSN: 0005-2736
    Keywords: (Vascular smooth muscle) ; A10 cell line ; Chloride radiolabel ; Chloride-bicarbonate exchange
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Analytical Biochemistry 50 (1972), S. 84-88 
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Reviews on Biomembranes 906 (1987), S. 277-294 
    ISSN: 0304-4157
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Chemistry and Physics of Lipids 17 (1976), S. 416-422 
    ISSN: 0009-3084
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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