ISSN:
1573-5001
Keywords:
Protein stucture determination
;
NMR structure
;
Assignment of NOE distance constraints
;
Structure refinement
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A new program, ASNO (‘ASsign NOes’), for computer-supported NOE cross-peak assignments is described. ASNO is used for structure refinement in several rounds of NOESY cross-peak assignments and 3D structure calculations, where the preliminary structures are used as a reference to resolve ambiguities in NOE assignments which are otherwise based on the chemical shifts available from the sequence-specific resonance assignments. The practical use of ASNO for proteins is illustrated with the structure determination of Dendrotoxin K from Dendroaspis polylepis polylepis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00174613
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