Publication Date:
1994-09-30
Description:
In a process called "molecular combining," DNA molecules attached at one end to a solid surface were extended and aligned by a receding air-water interface and left to dry on the surface. Molecular combing was observed to extend the length of the bacteriophage lambda DNA molecule to 21.5 +/- 0.5 micrometers (unextended length, 16.2 micrometers). With the combing process, it was possible to (i) extend a chromosomal Escherichia coli DNA fragment (10(6) base pairs) and (ii) detect a minute quantity of DNA (10(3) molecules). These results open the way for a faster physical mapping of the genome and for the detection of small quantities of target DNA from a population of molecules.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bensimon, A -- Simon, A -- Chiffaudel, A -- Croquette, V -- Heslot, F -- Bensimon, D -- New York, N.Y. -- Science. 1994 Sep 30;265(5181):2096-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut Pasteur, Paris, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7522347" target="_blank"〉PubMed〈/a〉
Keywords:
DNA/*analysis/chemistry
;
DNA, Bacterial/analysis/chemistry
;
DNA, Viral/analysis/chemistry
;
*Genetic Techniques
;
In Situ Hybridization
;
Sensitivity and Specificity
;
Silanes
;
Staining and Labeling
;
Surface Properties
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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