ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The polymerase chain reaction (PCR) DNA amplification method was used to identify oligonucleotide primers from a target gene, hns, to specifically detect SulmonelIu spp. in contaminated oysters. The primers for PCR amplification and a hybridizing oligonucleotide probe to detect the amplified DNA were specific for all Salmonella spp. tested. Modification of a procedure for extracting DNA from oyster tissue matrices followed by PCR amplification, and coupled with gene-probe hybridization detected 〈40 cells of seeded or naturally occurring Sulmonella spp./g of oyster meat samples without any enrichment step. The detection of SaZmonella spp. was reliable, sensitive, and required considerably less time than conventional microbiological culture methods.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1993.tb06146.x
Permalink