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  • 1
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    Investigation on probable Viral Agents associated with grass carp and silver carp mortality in Gilan province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Warm water aquaculture development along with import of eyed eggs, Broodstocks and ornamental fish also transferring of fingerlings and broodstocks from one province to another one has led to spreading of some unwanted diseases. According to the Gilan Fisheries Department report, mortality rate of silver carp(Hypophthalmichthys molitrix) in the summer of 2010 caused losing of silver carp production more than 1162 tons which was valued over than twenty-three milion rials that was increased to about 40 million rials in 1391. Also grass carp had a less mortality rate in some farms. Observed symptoms and severity of losses increased the probability of viral diseases such as spring viremia of carp, koi herpes virus and grass carp reovirus. In this research, totally 411 silver carp and grass carp with 5 to 2300 gram weight from 83 farms were sampled during 2011-2012. Clinical symptoms was different in various farms and subcutaneous hemorrhages on the flanks, red fin base or total fin and Red belly and operculum, mild exophthalmia, hemorrhaging in the abdominal cavity (yellow, transparent or red fluid) and petechial haemorrhages of skin and viscera and Ascites in abdominal cavity and some silver carps and grass carps were observed with palied liver and gill. In some cases swelling muscle was observed in Silver carp. Several fries with the severe emaciation were observed. Difference between clinical symptoms can indicate the presence of different causes of mortality in different farms. Various water colors from completely transparent to dark green, yellow or slightly brown observed in different pool, which shows the difference in overall culture management including water quality management. The minimum Water turbidity using secchi disk was 40 centimeters to one meter page in the fields. Source semi-deep wells or deep pools that were used as water source. Average water temperature, oxygen and pH in fileds 26/5-31 °C , 4/3-5/7 mg / L and 7/4-9/38. In order to virological surveys, sampling of internal organs, including kidneys, and spleen were done.and Homogeneous target tissues after spending 0/45 micron filter on EPC and BF2 cell line were inoculated to observe cell damage(CPE) in case of confirmation of antibody detection methods brilliant, RT-PCR and PCR done. In cell culture examination, a kind of cell damage (CPE-like changes) was observed after inoculating of 3 samples of Silver carp those were suspected to SVC, but in the second and third passages there were no sign of cell damage. It may be because of toxic effect of tissue filtrate on the cell monolayer. Concerning sample of Grass carp on two EPC and BF2 cells no evidence of cell damage was found. In indirect fluorescent antibody test, 72 hours after inoculation of tissues filtrates of of silver carp and grass carp samples on EPC, no positive reaction was observed. PCR and RT-PCR tests using specific primer pairs were done to test all of the silver carp and grass carp samples for SVC and KHV diseases Also for GCRV in grass carp samples. In addition, simultaneously commercial PCR kits applied for testing of SVC and KHV (IQ2000. Taiwan). The results of PCR and RT-PCR tests showed no evidence of robdovirus Carpio and grass carp reovirus RNA and no sign of koi herpesvirus DNA in tested samples. In conventional PCR and RT-PCR tests Using specific primer pairs none of positive bands related to SVCV(470 bp) and GCRV(292bp, 697bp and 320bp) were not confirmed in the tested samples. Also in electrophoresis of PCR products using IQ2000 kit revealed 471 bp and 640 bp negative bands in all samples and the positive bands were not observed. For confirmation of the results, three suspected samples were sent to Europe Union Reference Laboratory and none of mentioned diseases were confirmed. Simultaneously bacteriolocical examination applied for 26 using blood agar. In bacterial culture and related analyzes 6 isolates of Pseudomonas and 21isolates of Aeromonas hydrophila were detected from 26 farms while no virus was confirmed in the same samples. Based on the results of cell culture, PCR, RT-PCR and IFAT tests no one of SVCV, KHV and GCRV viruses were confirmed in tested samples. Therefore, the etiological reason of the mortalities in the tested farms was not viral diseases and should be prevent and control by biosecurity and health management in the farms.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: SVCV ; KHV ; GCRV ; Silver Carp ; Grass carp ; Mortality
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 86pp.
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  • 2
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    The feasibility of Gamasiab river area fish farming of rainbow trout (Onchorhynchus mikiss) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was carried out about 15 months to determine suitable locations for the development of aquaculture across the Gamasiab river of Hamedan province in 2007 - 2008. Physicochemical parameters and macro benthic invertebrates fauna15 and 13 staitions respectively every 45 days and seasonally fish identify in 8 stations were performed according standard methods. The average water temperature was 12.8±3.7 oC, minimum and maximum dissoled oxygen were 2.6 and 12.5 mg/lit respectively during the study. The pH range was between 7.5-8.8 , the minimum and maximum Ec was 250 and 750 µmos/cm in Mehr and Tir.The transparency range was recorded about 1-103 F.T.U. The uder factors such as amount of suspended materials: 0.01-0.1 mg/l, amount of BOD5: 0.05-6.2 mg/l and COD: 30 (minimum) and 70.57 (maximum) were recirded. The total solids material (T.S.S) were less than 1 mg/lit. Totally 37 groups of bentic organisms were identified which 11 groups of them were appertained to pollutionsensitive orders containing Ephemeroptera, Trichoptera and Plecoptera. During fish population studied 21 species belonging 5 family were identified. Based on obtained results of biological, ecological, economic and social parameters, 10 sites in 7 areas were suitable identified for fish rearing and 12 aquaculture programs (10 plan for fish rearing and 2 plan for increasing fish production of (Onchorhynchus mikiss).The minimum and maximum production capacity in mentioned places was stimated about 1675 and 3850 tons respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Feasibility ; Complexes of fish farms ; Biotic and Abiotic factors ; Onchorhynchus mikiss ; Rainbow trout
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 92pp.
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  • 3
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    Cage culture of tilapia in some water resources (farm and agriculture ponds) of Bafq, Iran (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Tilapia is the second cultured fish in the world. Up to 135 countries produces tilapia. Intensive culture systems of tilapia such as cage culture are recently developed because of limitations of water resources. In Iran, studies about tilapia were started in National Research Center of Saline Water Aquatics from November 2008 and some aspects of tilapia aquaculture and culture systems are surveyed. Investigation about cage culture of Nile tilapia, Oreochromis niloticus was performed in two culture periods of 2012 and 2014 in fresh and brackish water conditions of Iran for the first time. Stocking densities of fish in the cages were 25, 50, 75, 100, 125 and 150 fish/m^3 . 100g fish stocked reached 272-331 g during 80 days in the first study. However, 50g fish stocked reached 329-450 g during 145 days in the second study. Results of cage culture of tilapia in fresh and brackish water conditions showed ranges of FCR: 1.56-3.72 and 1.08-1.87, DGR: 1.63-2.91 and 2.14-2.92 g/day, SGR: 0.63-1.12 and 1.29-1.54, production: 5.98-38 and 7.17-41.6 kg/m^3 , respectively. Stocking densities 75-125 fish/ m^3 were appropriate especially in fish reared in brackish water cages.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Tilapia ; Cage ; Culture ; Density ; Oreochromis niloticus ; Nile tilapia ; Agriculture ponds
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 44pp.
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  • 4
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    Economic and social assessment of fish farming in cages in the Southern Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study aimed to assess the social, economic and fish farming in cages in the Southern Caspian Sea. The Caspian Sea is considered as one of the most important water resources of Iran, in which due to the appropriate weather conditions in the north of the country, has the potential for marine aquaculture. With the implementation of this plan would say that with respect to the proposed initial selection from Kheshtsar in Mazandaran province and Kiashahr in Guilan province with along coastline of about 250 km from the depth of 20 to 100 meters and an the area of about 2027km2 has the cage aquaculture talent. Of course, at the depths of greater than 100 M by using the world technology procedure, there is also the possibility of the establishment of marine cage aquaculture. Now, with the regards of the above potential in the Caspian Sea coastline, it is expected at least 200 cages fish sites farming in with the establishment of 40 cages per site and produce 160,000 tons with creating of 1,000 direct jobs and 2,000 indirect jobs and income 2000 billion gross annual average net profit of 40% could be 800 billion in which will produce of proteins needed part of the country, creating jobs, welfare, prosperity and economy in the region. With the implementation of such national project, it is needed to establish pilot farms to reach the goals: environmental assessment studies, risk assessment, aquatic selection and the use of appropriate cage structures according to ecological conditions of southern Caspian Sea.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Cage ; Economic activity ; Profitability ; Rainbow trout ; Ecological conditions
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 36pp.
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  • 5
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    Comparison study on different synthetic hormones used for artificial propagation of Stellate Sturgeon Acipenser stellatus stellatus Berg, 1932 (2018)
    Details
    Publication Date: 2021-05-19
    Description: In this study the effects of LHRHa, a mixture or common carp and sturgeon pituitary (CCP +SPE), Thyroxin hormone (T3) with commonly method used in hatcheries were compared. The experiments were conducted using different doses of hormones with several replicates in order 10 determine the most effective dose. Total number of broodstocks were females and 22 males. Prior to injecting the fishes, the Germinal Vesicle (GV) situation of all broodstocks were determined. The results showed that bloodstocks has been injected with LHRHa were very satisfactory and the percentage of response, fertilization and hatching of them had significantly different. The least effective dose of LHRHa that induced maturation was single injection and 350-375µg/fish for double injection. Thus it was determined that under the experimental condition, 20.0 to 31.2 µg/Kg of body weight of broodstock was the most effective dose. In the case of male broodstocks LHRHa was the most effective hormone and the sperms collected from these broodstocks were better in quality and quantity. The most effective dose of this hormone for males was determined to be 200 µg/fish or 27.7 to 31.2 µg/kg of body weight. The best dose of mixture of CCP + SPE which all broodstocks responded was7.0mg/kgBW +2.5mg/kgBW for females and 7.0mg/kgBW+ 1.5mg/kgBW for males. In the case of broodstocks that were first injected thyroxin and latter injected SPE it must be stated that since the thyroxin used was edible grade none of the broodstocks attained maturity. Therefore the results obtained in this treatment were not satisfactory.
    Description: Published
    Keywords: Synthetic hormones ; Stellate sturgeon ; Acipenser stellatus stellatus ; LHRHa ; Pituitary ; Thyroxin hormone ; Germinal Vesicle ; GV ; Fertilization ; Maturation ; Sturgeon ; Common carp ; Hormone
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.9-24
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  • 6
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    Aquaculture Potentials Investigation of Yamchi Dam Down Stream Areasin in Ardabil Province (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The research conducted for finding of aquaculture potential and appropriate areas of Yamchi dam downstream river and estimate of its production in 2010-2012. Yamchi dam is located at 25 kilometers of southwest of the Ardabil city. This research done by using of available information and data obtained from the monthly and seasonally sampling. After evaluating of Yamchi river potential and its marginal lands, suitable locations for aquaculture determinated and theirs production was calculated at each site. Totally, 3 areas selected for trout aquaculture and varius method suggested including race way, octagonal pond and semi-recircultaion. In the present condition of Yamchi river the amount of trout production is predicted at least 65 ton by race way to maximum 1750 ton by semirecircultaion method. Whiles, if 2 cubic meters of water from Yamchi dam outlet is provided to aquaculture, it will be predicted at least 580 ton by race way to maximum 4000 ton by semi-recircultaion method
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Feasibility ; Aquaculture ; Balakhli chai
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 49pp.
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  • 7
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    Rearing of Indian Craps fries to 1g weight in earthen ponds (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: 30000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (2007 and 2008) and transferred to the earthen ponds in Astaneh Fisheries Research Station (Gilan ) and Sheiban Resaerch center (Khozestan) , In order to assess the viability of rearing fry up to 1 gram fingerlings under the climatic condition of north and south part of Iran . Growth parameters from the larval stage to 1 g were studied also .The fry with 300 mg weight, were released in to 3ponds.specimens were separated to tree experimental categories. fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured . Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings.Catla was gain 1 g. during 15 day while Roho and Merigal were gain 1 g. during 14 and 10 days respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility specialy in other provinces like Sistan and Baluchestan were remind for futhre studies.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Juvenile ; Earthen ponds ; Fisheries ; Fry ; Fingerlings ; Artificial food ; Oxygen level ; Turbidity ; Fertilizers
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 8
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    Preparation instructions micro-algae cultivate and concentrate it for use in silver carp feed and training necessary (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: According to the importance of micro-algae in aquatic feed such as fish in this study have been investigated Preparation of useful algae powder or concentrate for breeding silver carp , their impact on the growth of silver carp , rate per unit area and estimate n economic Development. Different algal species were isolated from hydrothermal fish farms, then were purified and mass culture . The next step microalgae were dried and powdered by spray dryer and were examined the fish feeding on them. During this study, 6 species of chlorophyt( Scenedesmus obliquus, Scenedesmus acuminatus, Chlorella vulgaris, Pediastrum boryanum, Pandorina morum, Ankistrodesmus falcatus) ,3 species of cyanophyta ( Anabaena flosaquae, Oscillatoria agardhi and Spirulina platensis) and 1 species of Bacillariophta ( Cyclotella meneghiniana were isolated from.Green algae and Blue -green algae were cultured in Zaindr medium, diatoms were cultured in Zaindr medium but with water of Anzali logoon and also in F2 medium with artificial sea water and spirulina was cultured in Zarouk medium. Microalgae were cultures then concentrated.Then the impact was examined on fish silver carp 2 to 3 grams. The results showed that Cyclotella has a greater role in the growth of silver carp and Anabaena floes aquae and Spirulina platensis tend to growth less than cyclotella. Scenedesmus obliquus and Scenedesmus acuminatus were respectively next algae that showed the greatest impact on fish growth. Scenedesmus obliquus feed rate was greater than any other algae for Daphnia.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Silver carp ; Algal concentrate ; Micro-algae ; Scenedesmus obliquus ; Scenedesmus acuminatus ; Chlorella vulgaris ; Pediastrum boryanum ; Pandorina morum ; Ankistrodesmus falcatus ; Spirulina platensis ; Oscillatoria agardhi ; Cyclotella meneghiniana ; Preparation ; Feed
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 73pp.
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  • 9
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    The study and feasibility of Cheshmeh-Ali River and down stream area of Shahid Shah-Charaghy Dam in Damghan for aquaculture development (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This research was conducted to finding of Cheshmeh-Ali and Shahcheraghi-Dam downstream areas potential for developing of aquaculture industry. The present study was conducted based on available information and data from monthly and seasonally sampling including geographical area, margin lands and fluctuations of river water, seasonal climatic changes view point of rainfall, temperature, wet, evaporation, wind velocity, sunshine hours, frost and also chemical and physical parameters of water including temperature, alkalinity, total hardness, chloride, calcium, magnesium, sodium, potassium, organic phosphorous and nitrogen, suspended solids, turbidity, water flow and flood conditions, and in addition phytoplankton and zooplankton communities, macrobenthic invertebrates, fishes, aquatic plants, amphibians, reptiles, birds. Results showed that the upstream and downstream regions of Shahcheraghi dam are not suitable for warm water aquaculture in earthen ponds. But view point of cold-water aquaculture, particularly trout, there are some good situations. Also it can be suggested different culture systems including of race way, octagonal concrete ponds, semi-circulation and circulation methods. But, due to water restrictions in the area, semi-circulation and circulation system is preferred. In ideal conditions can be produced about 1,600 tones of trout, if all the capacity of this area be used for aquaculture. Otherwise, in limited circumstances view point of exploitation possibility of the water, only about 700 tons can be produced. In addition, there were appropriate facilities in spring area of Cheshmehali for tourism industry, that it can be considered as priorities for economic development in the region. Due to the low sensitivity of the ecologically conditions, native or exotic aquatic species rearing in indoor methods can be recommended.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemical ; Physical ; Site selection ; Aquaculture capacity ; Sampling ; Temperature ; Turbidity ; Culture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 71pp.
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  • 10
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    Determination of optimum Dosage of Ovaprim injectionon Artificial spawning Efficiency of Esox Lucius (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This project was conducted to goal of optimum dosage determination of ovaprim injection to artificial spawning efficiency of Esox lucius. The research implemented by 4 treatments with 3 replicates for each ones. 3 female and 6 male brooders injected in each replicate. The animals in 1, 2 and 3 treatments injected by 10, 20 and 30 µg/kg BW, respectively, and 4th treatment as a control injected with 4 mg/kg BW pituitary gland extract. Average weight of brooders were 1361±521, 1376±954, 1009±160 and 1100 ±422 g in 1, 2, 3 and 4 treatments in females and 689±145, 734±197, 547±118 and 794±238 g in males, respectively. In addition, positive response percent to hormone injection were measured 77.8 ±19.24 , 88.9 ± 19.24 , 55.5 ±50.91 and 55.5 ± 19.24 % in 1, 2, 3 and 4 treatments in female and 94.4 ± 9.58, 88.9 ±19.26 , 83.3±28.86 and 88.9 ± 19.26 % in male brooders, respectively, but there was no significant different between all of treatments (p〈0.05). Incubation period from fertilization till hatching step in 7 to 15 ˚C was 5 to 10 days with average of 7±1.5 days. Fertilization content was in 1 to 4 treatments measured 87.1±10, 88.04±7.7, 83.9±5.2 and 72.4±19.7 %, respectively and also the treatments didn’t show any different significantly together (p〈0.05). Average percentage of eyed eggs 66.6±15.9 in treat 1, 61.2±22.3 in treat 2, 58.3±10.7 in treat 3 and 56.1±15.04 in treat 4, without any significant different between of them (p〈0.05). Hatching of eggs mean were measured 27.41±19.8 in treat 1, 39.53±26.9 in treat 2, 95.18±5.6 in treat 3 and 26.78±12.4 in treat 4, and significant different observed between of them too (p〈0.05).In the other hand, mean percent of larvae with active feeding in these treatments were measured 18.77±14.6, 20.1±8.51, 55.6±11.6 and 14.51±7.72 as the treatments had significant different (p〈0.05). Also, the best temperature and dosage injection of ovaprim hormone was 9 to 12.5 ˚C and 20µg/kg BW, respectively.The end of trial, from 103740 larvae introduced to earthen pond obtained 8000 fingerlings with weight of 2.68±0.6 g and length of 6.96±0.51 cm.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Esox Lucius ; Reproduction ; Spawning ; Ovaprim Injection ; Fecundations ; Hatching ; Brooders ; Females ; Males ; Fertilization ; Hormone ; Larvae ; Earthen pond ; Fingerlings
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 168pp.
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