ISSN:
1612-1112
Keywords:
High performance gel permeation chromatography
;
Liquid scintillation counter
;
On-line detection
;
Fructan oligomers
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary 1-F-Fructan/1-F-Fructan-Fructosyltransferase (FFT, EC 2.4.1.100) catalyses the transfer of a single fructose residue of an inulin-type fructan as donor to an acceptor. The donor must have a degree of polymerisation (DP) of at least 3 and the acceptor a DP of at least 2. High Performance Gel Permeation Chromatography (HPGPC) combined with an on-line Liquid Scintillation Counter was employed as an efficient and quick method for the separation and detection of the oligosaccharides produced by the catalytic action of FFT. Quantification of specific enzymatic activity and its selective detection was achieved by the use of radioactive substrates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02268181
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