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  • 1
    Unknown
    Chantilly, Va. : Mineralogical Society of America
    Description / Table of Contents: Carbon in Earth is an outgrowth of the Deep Carbon Observatory (DCO), a 10-year international research effort dedicated to achieving transformational understanding of the chemical and biological roles of carbon in Earth (http://dco.ciw.edu). Hundreds of researchers from 6 continents, including all 51 coauthors of this volume, are now engaged in the DCO effort. This volume serves as a benchmark for our present understanding of Earth's carbon - both what we know and what we have yet to learn. Ultimately, the goal is to produce a second, companion volume to mark the progress of this decadal initiative. This volume addresses a range of questions that were articulated in May 2008 at the First Deep Carbon Cycle Workshop in Washington, DC. At that meeting 110 scientists from a dozen countries set forth the state of knowledge about Earth's carbon. They also debated the key opportunities and top objectives facing the community. Subsequent deep carbon meetings in Bejing, China (2010), Novosibirsk, Russia (2011), and Washington, DC (2012), as well as more than a dozen smaller workshops, expanded and refined the DCO's decadal goals. The 20 chapters that follow elaborate on those opportunities and objectives. A striking characteristic of Carbon in Earth is the multidisciplinary scientific approach necessary to encompass this topic. The following chapters address such diverse aspects as the fundamental physics and chemistry of carbon at extreme conditions, the possible character of deep-Earth carbon-bearing minerals, the geodynamics of Earth's large-scale fluid fluxes, tectonic implications of diamond inclusions, geosynthesis of organic molecules and the origins of life, the changing carbon cycle through deep time, and the vast subsurface microbial biosphere (including the hidden deep viriosphere). Accordingly, the collective authorship of Carbon in Earth represents laboratory, field, and theoretical researchers from the full range of physical and biological sciences.
    Pages: Online-Ressource (xv ; 698 Seiten)
    ISBN: 9780939950904
    Language: English
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Microbial ecology 18 (1989), S. 29-44 
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antibiotic-insensitive mutants of natural sedimentary bacteria from an intertidal site were selected on gradient plates. Two of these strains, anAeromonas sp. andVibrio alginolyticus, were mixed with natural sediments from the field and fed toAbarenicola vagabunda, an intertidal lugworm characteristic of sandy beaches in the Pacific Northwest. Digestive removal was apparent in the midgut, 97% efficiency being seen forAeromonas sp. Both strains showed rapid growth in the hindgut, increasing between 2 and 3 orders of magnitude in abundance between the midgut and rectum of the polychaete, corresponding with a doubling time of about 50 min for each strain. Direct epifluorescence counts of natural bacteria in guts of animals freshly collected from the field suggest a mean doubling time that is only slightly greater (66 min) for all ingested bacteria that survive midgut digestion. These bacterial growth rates exceed by orders of magnitude the greatest rates reported for ambient marine sediments and suggest that hindgut bacterial growth, though of little immediate importance in the energetics of the animals, may strongly influence both population dynamics of marine bacteria and diagenesis of sedimentary organic matter.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-072X
    Keywords: Archaea ; Hyperthermophile ; Hydrostatic pressure ; Pyrococcus ; Deep-sea vent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel, hyperthermophilic, anaerobic, sulfurmetabolizing archaeon was isolated from a fluid sample from recently discovered hydrothermal vents in the North Fiji basin (SW Pacific), at 2000 m depth. The new organism, strain GE5, is a gram-negative, highly motile coccus. It grows between 67° and 102°C under atmospheric pressure, with an optimum at 96°C (doubling time 33 min). The upper growth temperature is extended by at least 3°C when cells are cultivated under in situ hydrostatic pressures (20 MPa). Strain GE5 is an obligate heterotroph, fermenting peptides, or mixtures of amino acids to acetate, isovalerate, isobutyrate, propionate, H2 and CO2. Hydrogen inhibits growth unless sulfur is present. In the presence of sulfur, H2S is then produced. Phylogenetic analyses of the 16 S rRNA sequence of strain GE5 places the new isolate within the Thermococcales. By its high growth temperature and physiological features the new isolate ressembles Pyrococcus sp. However it deffers by a 7% mol upper G+C-content and shows low level of DNA similarity with the two previously described species. Based on these differences the description of strain GE5 as a new species Pyrococcus abyssi (CNCM I-1302) is proposed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Earth and Planetary Sciences 30 (2002), S. 385-491 
    ISSN: 0084-6597
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Geosciences , Physics
    Notes: Abstract The recent recognition of a potentially vast, unexplored hot microbial biosphere associated with active volcanism along the global mid-ocean ridge network has fundamentally shifted concepts of how planets and life coevolve. Many processes intrinsic to the dynamics of the spreading center volcanic system provide partial or complete nutritional fluxes that support diverse microbial communities that thrive under extreme conditions on and beneath the seafloor. Mantle melting, volcanism, and fluid-rock reactions transport volatiles from the asthenosphere to the hydrosphere. Volcanic heat and exothermic reactions drive circulation of nutrient-rich fluids from which chemosynthetic organisms gain metabolic energy. In turn, many of these organisms symbiotically support macrofaunal communities that populate the vents. Long-term seafloor observatories will allow exploration of linkages between volcanism and this newly discovered biosphere. Such approaches may provide essential new information about our own planet while providing critically needed insights into how we can explore other planets for life.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 217 (1968), S. 1263-1264 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The first reported isolation of F. parahaemolyticus-like organisms in the United States was from Puget Sound and Washington Coast sediments4. Approximately 10 per cent of the vibrios isolated from these sources showed characteristics similar to those described for F. parahaemolyticus. In this ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 101 (1992), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Lipid analysis of a preliminary sampling of the Endeavor Ridge hydrothermal vent site was performed in order to estimate the extent and nature of the diversity of the microbial community. The vent microbial community was found to be highly variable in density and composition. Evidence was found for a dense microbial community of archaebacteria and possibly Thiobacilli in the interior of the flange of a black smoker, a red Beggiatoa-type colony in a sediment sample, and large amounts of polyenoic fatty acids of the type previously found in barophilic eubacteria. Lipid analysis provided a ‘snapshot’ of the in situ biomass, community structure, and metabolic status of the microbial community, a valuable addition to the techniques available to the microbial ecologist.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 31 (2000), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: PCR-amplified 16S rRNA genes from particle-attached and free-living Archaea in the Columbia River estuary, particle-attached Archaea in the river, and Archaea in the adjacent coastal ocean were cloned, and 43 partial sequences were determined. There was a high diversity of Archaea in the estuary, especially among the particle-attached Archaea, with representatives from four major phylogenetic clusters. Eighteen of 21 estuarine clones were closely related to clones from the river and the coastal ocean or to clusters of marine and soil clones identified in other studies. This contrasts with a similar study of the estuarine bacterial community that found 62% of bacterial 16S rRNA clones to be unique to the estuary. Archaea in the estuary were primarily allochthonous, and therefore, unlike the bacteria, probably do not form a native estuarine community.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 25 (1998), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Thermophilic and hyperthermophilic microorganisms were cultured from 18°C diffuse hydrothermal fluids at the CoAxial segment deep-sea hydrothermal vent site 3 months after an eruption resulting from an intrusion of magma into shallow crust. The abundances of these organisms decreased over a 3-year period as the shallow magma cooled. The presence of these organisms at the site suggests that these organisms grew in response to nutrient input from hydrothermal fluid circulation and then were flushed to the surface following the eruption. Thermophiles and hyperthermophiles were also found in low-temperature (3–30°C) fluids at three other chronic, highly active deep-sea vent sites. The origin of these organisms is not known but may include the overlying seawater or a shallow to deep subseafloor habitat.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 32 (2000), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The abundance of halotolerant microorganisms in hydrothermal-vent and pelagic waters in the North and South Pacific was estimated by the most probable number (MPN) technique using a heterotrophic 16% NaCl medium incubated at 20–24°C. Based on these MPNs and direct counts with epifluorescence microscopy to enumerate the total microbial population, salt-tolerant microbes comprised from 〈0.01 to 〉28% of the total microbial community. Fourteen isolates from these MPN enrichments were identified by sequencing a portion of the 16S rRNA gene, and all were found to belong to the genera Halomonas and Marinobacter. The response to salt of mesophilic hydrothermal-vent microbial isolates obtained without selecting for salt tolerance was also examined. Forty-one of 65 strains cultured from hydrothermal plume waters, low-temperature hydrothermal fluids, sulfide rock and an animal specimen at ∼2000–2200 m depth from the Endeavour Segment of the Juan de Fuca Ridge were subjected to increasing concentrations of NaCl, and over half grew at a NaCl concentration that is lethal to many commonly isolated marine bacteria. At least 36 of the 65 isolates (≥55%) grew in the enrichment medium supplemented with 10% NaCl; at least 30 of 65 (≥46%) grew with 16% NaCl; at least 20 of 65 (≥31%) tolerated 22% NaCl. Based on phylogenetic analysis of the 16S rRNA gene in nine of these 65 isolates, four belonged to the genus Halomonas. These Halomonas strains tolerated 22–27% NaCl. It is possible that a majority of the other 16 isolates which grew with 22% NaCl are also Halomonas based on their degree of halotolerance, morphology, and apparent abundance as revealed by MPN enrichments. The four Halomonas strains obtained without selecting for halotolerance were further characterized physiologically and metabolically. Overall, they grew between −1°C and 40°C, were facultative aerobes, oxidized between 49 and 70 organic compounds according to Biolog plate substrate utilization matrices, grew with oligotrophic quantities of carbon (0.002% yeast extract) in liquid media, reduced nitrate to nitrite, and tolerated up to 0.05–3 mM Cd2+. Halomonas is one of the most abundant culturable organisms in the ocean, and its success may be attributed to its metabolic and physiological versatility.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Eight new strains of deep-sea hyperthermophilic sulfur reducers were isolated from hydrothermal vent fields at 9°50′N East Pacific Rise (EPR) and at the Cleft and CoAxial segments along the Juan de Fuca Ridge (JdFR). 16S rRNA gene sequence analysis showed that each strain belongs to the genus Thermococcus. Restriction fragment length polymorphism patterns of the 16S/23S rRNA intergenic spacer region revealed that these isolates fell into three groups: those from the EPR, those from fluid and rock sources on the JdFR, and those isolated from Paralvinella spp. polychaete vent worms from the JdFR. The optimum-temperature specific growth rates and the temperature ranges for growth were significantly higher and broader for those strains isolated from worms relative to those isolated from low-temperature diffuse hydrothermal fluids. Furthermore, the worm-derived isolates generally produced a larger array of proteases and amylases based on zymogram analyses. The zymogram patterns also changed with growth temperature suggesting that these organisms alter their lytic protein suites in response to changes in temperature. This study suggests that there is significant phenotypic diversity in Thermococcus that is not apparent from their highly conserved 16S rRNA nucleotide sequences.
    Type of Medium: Electronic Resource
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