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  • 1
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We analysed mitochondrial DNA (mtDNA) variation of lake sturgeon (Adpenser fulvescens) from the Moose River basin. Our objective was to address various proximate and ultimate factors which may influence the distribution of lake sturgeon mtDNA haplotype lineages in this watershed. The lake sturgeon sampled were characterized by only two mtDNA hapiotypes based on a restriction fragment length polymorphism analysis with 40 restriction endonucleases and direct sequencing of 275 nucleotides in the mtDNA control region. We detected no heterogeneity in the mtDNA haplotype frequencies of lake sturgeon captured from different sites within rivers including those separated by major hydroelectric installations. However, lake sturgeon from one tributary had significantly different haplotype frequencies than those from other tributaries suggesting that they composed a discrete genetic stock. These results suggest that gene flow among most sites is significant and is an important factor affecting the distribution of mtDNA variation in this species. The genetic structuring and diversity are discussed in relation to lake sturgeon management and conservation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 47 (1995), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We have isolated specific microsatellite loci from a partial genomic library of brook charr Salvelinus fontinalis. Their usefulness was investigated by measuring intra- and inter-population genetic diversity at four loci among 20 individuals from each of five lakes located 3 to 22 km apart in La Mauricie national park (Canada). These markers were moderately to highly polymorphic. A total of five, six, 16 and 18 alleles per locus were detected, and their overall expected heterozygosity was 0.53, 0.58, 0.86 and 0.87. Strong inter-population diversity was detected. Highly significant differences in allelic frequencies were found in all but two pairwise χ2 permutation tests between populations at all loci. Numerous population unique alleles were observed in all five lakes. Consequently, a highly significant component of total genetic diversity was due to interpopulation variance, as exemplified by GST values of 0.33, 0.42, 0.47 and 0.84 for each individual locus. Altogether, the results indicated that these loci should be valuable in addressing fine scale population genetics questions in brook charr. To our knowledge, they also represent the first available microsatellites developed in the genus Salvelinus.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 35 (1989), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: This study was designed to test the hypothesis that anadromous populations of North American whitefish, Coregonus clupeaformis (Mitchill), believed to have suffered more intense population bottlenecks during past glaciation events, should reveal less mitochondrial DNA (mtDNA) variability and population-genetic structuring than anadromous populations of European whitefish, C. lavaretus (L.). C. clupeaformis exhibited extremely low levels of diversity and population structuring, in terms of number and frequency of clonal lines as well as sequence divergence estimates, as compared with populations of C. lavaretus. These results support the hypothesis that the severity of population bottlenecks related to Pleistocene glaciation events may be largely responsible for the level of mtDNA variability observed. This in turn influences the sampling strategy required to maximize the usefulness of mtDNA analysis in stock discrimination.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Larvae of anadromous rainbow smelt originating in various spawning tributaries are retained in the St Lawrence estuary. We proposed that these smelt represent one population genetically differentiated from adjacent populations characterized by geographically distinct larval retention areas. We also analysed four landlocked populations to evaluate the phylogenetic basis of dwarf and normal-sized phenotypes and their relation to an adromous smelt. A phylogenetic distinction was revealed between anadromous and landlocked smelt, with only one of the two mtDNA phylogenetic groups of anadromous fish observed among landlocked smelt. Significant geographical heterogeneity in the distribution of mtDNA genotypes was observed among landlocked smelt, suggesting that dwarfism in smelt may be polyphyletic in origin. St Lawrence smelt were genetically identical but distinct from adjacent populations, supporting the proposition that population genetic structure reflects the number of larval retention zones rather than spawning sites.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 39 (1991), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Mitochondria1 DNA (mtDNA) restriction analysis was used to assess phylogenetic patterns among 21 taxa of the subfamily Coregoninae. The genus Prosopium formed a very distinct group differing by 10% (sequence divergence estimate) from other species. Coregonus and Stenodus species were closely related, diverging by sequence divergence estimates of less than 5.6%. These species split into two major sister groups. One comprised all ‘true whitefish’ (subgenus Coregonus) and four cisco species (subgenus Leucichrhys). The most distant species within this assemblage was the Acadian whitefish (C. huntsmani). The other group included all other cisco species and also the Inconnu (Stenodus leucichthys). These results supported a polyphyletic origin of the ciscoes, and did not support Stenodus as a sister taxon of the genus Coregonus. The levels of sequence divergence observed suggested that most extant coregonines radiated during the Pleistocene.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 47 (1995), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: This study investigates the patterns of genetic diversity detected in allozymes, mtDNA, and microsatellites, in order to assess their relative efficacy to differentiate sympatric landlocked salmon populations and to estimate changes in genetic diversity between wild and first-generation hatchery fish. Overall, the three genetic markers indicated a genetic differentiation between two sympatric populations of Lake Saint-Jean, Québec. MtDNA and microsatellites also showed significant differences between wild and first-generation hatchery fish originating from the same river. Allozyme analysis was the most limited approach due to the low genetic diversity detected and the necessity to kill specimens. Although low polymorphism was found in mtDNA, it was the most discriminant marker between wild populations. Microsatellite analysis appears to be a promising approach due to its high sensitivity in differentiating wild populations, in detecting changes in allele composition between wild and first-generation hatchery fish and its potential for increased resolution by augmenting the number of polymorphic loci. Given the benefits and disadvantages of the three methods, the combination of mtDNA and microsatellite analyses will best address our research objectives.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 54 (1999), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Genetic data suggest that the littoral and pelagic forms of brook charr Salvelinus fontinalis in Lake Bondi are two populations with partial reproductive isolation and non-random mating. Genetic differentiation between the two groups was supported by differences in allele frequencies and by deviation from Hardy-Weinberg equilibrium when the two groups were pooled; no such deviation was observed when fish were divided into littoral and pelagic groups. In contrast to Lake Bondi, no clear evidence of genetic differentiation was observed in Lake Ledoux. Discriminant function analyses of morphological characters support the existence of littoral and pelagic groups in Bondi and Ledoux Lakes. In Lake Bondi, the two groups differed significantly in two shape variables (pelagic fish had shorter dorsal fins, and longer body length posterior to the dorsal fin than littoral ones) whereas in Lake Ledoux, the groups differed in four shape variables (pelagic fish had shorter pectoral fins, shorter dorsal fins, and a shorter and higher caudal peduncle than littoral ones). Discriminant analyses of these characters were effective in reclassifying fish into their appropriate groups in both populations, with an efficiency of 78% for juveniles in Lake Bondi and 69% for adults in Lake Ledoux. Differences in morphology between the two forms are consistent with adaptations required to forage in each zone, i.e. benthic form in the littoral zone and planktivorous form in the pelagic zone.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 53 (1998), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The abundance, spatio-temporal distribution, and feeding of larvae were compared from two reproductively isolated dwarf and normal ecotypes of whitefish (Coregonus sp.) to test the hypothesis that larval ecotypes should reduce competition by using different resources. Contrary to a priori expectations, trophic niche partitioning between larval populations was much less pronounced than previously reported for the adult stages of fish ecotypes, presumably due to the lack of competition acting at this stage, and related to non-limiting food resources. Because this study was conducted in a single year and a single lake, we cannot however, strictly rule out the possibility that resource-based competition and resulting niche partitioning may occur at the larval stage in these species complexes. Nevertheless, this suggests for the first time, that resource-based selection at the larval stage may be relatively unimportant compared to that occurring at older life-history stages in driving the divergence and the development of reproductive isolation in sympatric fish ecotypes.
    Type of Medium: Electronic Resource
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  • 9
    Publication Date: 2021-05-19
    Description: Underground environments are increasingly recognized as reservoirs of faunal diversity. Extreme environmental conditions and limited dispersal ability of underground organisms have been acknowledged as important factors promoting divergence between species and conspecific populations. However, in many instances, there is no correlation between genetic divergence and morphological differentiation. Lucifuga Poey is a stygobiotic fish genus that lives in Cuban and Bahamian caves. In Cuba, it offers a unique opportunity to study the influence of habitat fragmentation on the genetic divergence of stygobiotic species and populations. The genus includes four species and one morphological variant that have contrasting geographical distributions. In this study, we first performed a molecular phylogenetic analysis of the Lucifuga Cuban species using mitochondrial and nuclear markers. The mitochondrial phylogeny revealed three deeply divergent clades that were supported by nuclear and morphological characters. Within two of these main clades, we identified five lineages that are candidate cryptic species and a taxonomical synonymy between Lucifuga subterranea and Lucifuga teresinarum. Secondly, phylogeographic analysis using a fragment of the cytochrome b gene was performed for Lucifuga dentata, the most widely distributed species. We found strong geographical organization of the haplotype clades at different geographic scales that can be explained by episodes of dispersal and population expansion followed by population fragmentation and restricted gene flow. At a larger temporal scale, these processes could also explain the diversification and the distribution of the different species.
    Description: Los ambientes subterráneos están cada vez más reconocidos como reservorios de la diversidad de fauna. Las condiciones ambientales extremas y capacidad de dispersión limitada de los organismos subterráneos han sido reconocidos como factores importantes la promoción de la divergencia entre las especies y poblaciones de la misma especie. Sin embargo, en muchos casos, no existe una correlación entre la divergencia genética y la diferenciación morfológica. Lucifuga Poey es un género de peces stygobiotic que vive en las cuevas de Cuba y las Bahamas. En Cuba, ofrece una oportunidad única para estudiar la influencia de la fragmentación del hábitat sobre la divergencia genética de stygobiotic especies y poblaciones. El género incluye cuatro especies y una de las variantes morfológicas que han contrastar las distribuciones geográficas. En este estudio, en primer lugar se realizó un análisis filogenético molecular de las especies cubanas Lucifuga utilizando marcadores mitocondriales y nucleares. La filogenia mitocondrial reveló tres subtipos profundamente divergentes que fueron apoyadas por caracteres morfológicos y nuclear. En dos de estos clados principales, se identificaron cinco linajes que son especies crípticas y candidato taxonómica una sinonimia entre Lucifuga subterranea y Lucifuga teresinarum. En segundo lugar, Los análisis filogeográficos utilizando un fragmento del gen del citocromo b se realizó para Lucifuga dentata, el más distribuidas las especies. Se encontró una fuerte organización geográfica de los subtipos de haplotipos en diferentes áreas geográficas escalas que se pueden explicar por episodios de dispersión y expansión de la población seguido por la población la fragmentación y el flujo genético restringido. En una escala temporal más amplio, estos procesos también pueden explicar la diversificación y la distribución de las diferentes especies.
    Description: Published
    Description: peces ciegos, especies crípticas, cuevas,
    Keywords: Fish ; Phylogeny
    Repository Name: AquaDocs
    Type: Journal Contribution , Non-Refereed , Article
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  • 10
    Publication Date: 2011-06-13
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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