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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Environmental science & technology 11 (1977), S. 913-916 
    ISSN: 1520-5851
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Analytical chemistry 55 (1983), S. 1731-1734 
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 158 (1992), S. 267-270 
    ISSN: 1432-072X
    Keywords: Nitrosomonas europaea ; anaerobic metabolism ; nitrite respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrosomonas europaea is capable of maintaining an anaerobic metabolism, using pyruvate as an electron donor and nitrite as an electron acceptor; utilization of nitrite depends upon supply of both pyruvate and ammonia. The role of ammonia in this reaction was not determined. N europaea also assimilates CO2 anaerobically into cell material in the presence of nitrite (0.5–1.0 mM), pyruvate and ammonia. This reaction was partially inhibited by nitrite which apparently competed with CO2 for reducing power. Anaerobic “nitrite respiration” is sensitive to ionophores, FCCP being the most effective.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 120 (1994), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A unique genomic DNA fragment was isolated from Nitrosomonas europaea ATCC 19718. Based on the sequence of this fragment, oligonucleotide primers for polymerase chain reaction amplification were prepared which amplify sequences of 775 and 658 bp. The predicted DNA fragments were both amplified from the genome of N. europaea and a Nitrosomonas spp. isolated from a local oxidation pond. The primers failed to amplify DNA from the genomes of the ammonia oxidiser Nitrosolobous multiformis, the nitrite oxidiser Nitrococcus mobilis as well as from the genomes of other unrelated heterotrophic bacteria. These DNA sequences could be amplified from 0.01 ng of N. europaea genomic DNA or from 100 intact cells, and it was possible to detect Nitrosomonas DNA in a DNA mixture extracted from water samples drawn from a local oxidation pond.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 137 (1996), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at −35 and −10 have low similarity to the −10 / −35 consensus sequence of known prokaryotic promoters.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 21 (1996), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Microbial crusts are present on surfaces of soils throughout the world. A key feature of these crusts in arid zones is the abundance of filamentous sheath-forming and polysaccharide-excreting cyanobacteria. Several isolates of cyanobacteria were prepared from crust samples (Nizzana sand dunes, north-western Negev Desert, Israel). Optimal growth conditions for two such isolates of Microcoleus sp. were defined, and the role of the excreted polysaccharides in affecting the hydrological properties of crust-covered sand dunes was studied. Experiments with the native crust microbial population demonstrated the possibility of net primary productivity at both high relative air humidities and low moisture content.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 14 (1994), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract This paper describes the microbial ecosystem found on the leaves of Atriplex halimus, a salt-excreting plant in the central Negev highlands of Israel. Because of the regular nightly occurence of dew at this location, these leaves undergo a diurnal wetting so that phylloplane microorganisms experience large fluctuations in salinity and water activity, as well as tolerate repeated desiccation. During the dry season, in the late spring and summer, a significant amount of salts and organic material coats the leaf surface. During dew events the salt concentration at the leaf surface was calculated to be 〉 0.4 M. Direct counts of the respiring bacteria on the leaf surface ranged from 1.06×104 to 5.06×105 per cm2. Using a variety of media it was shown that there was limited bacterial diversity which could be cultured, with greater than 90% of the isolates being orange colored Gram-negative rods. Viable counts ranged from 0.32 to 2.32×104 bacteria per cm2 of A. halimus leaf surface. No bacteria capable of nucleating ice were recovered in these studies. The dominant orange pigmented bacterium, identified as a halotolerant Pseudomonas sp., grew optimally at 30°C and at 5% NaCl and was capable of growth in media containing up to 20% NaCl. This bacterium could grow on a variety of organic compounds, including some associated with plant materials. The leaf bacteria were desiccation-tolerant when on the leaf surface or when directly washed off the leaves, but much less so when in isolatd culture. A major component of the tolerance to desiccation is probably related to the compounds on the leaf surface.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 3 (1992), S. 255-264 
    ISSN: 1572-9729
    Keywords: ammonia ; nitrification ; nitrogen cycling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the sequence of events leading from ammonia to N2 during the process of biotransformation of inorganic nitrogen compounds, the weakest link, with respect to our knowledge and understanding of the organisms involved, is nitrification. In particular, this is true for the oxidation of ammonia to nitrite. The enzymes have not been thoroughly studied, and the enzymatic mechanisms have not been identified. Almost any biochemical and physiological aspect studied proved to be controversial, and major ecological questions still remain unanswered. Unless the structure and function of the various components of the process are worked out, progress in developing means for controlling nitrification will depend mainly on laborious trial and error and not on knowledgeable manipulation of this group of bacteria.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 8 (1997), S. 357-361 
    ISSN: 1572-9729
    Keywords: alkylpyridine ; subsurface bacteria ; biodegradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ten bacterial strains were isolated fromalkylpyridine polluted sediments 7.6 m below thesurface. These strains were able to degrade 11different alkylpyridine isomers. Degradation ratesdepended on number and position of the alkyl group. Isomers with an alkyl group at position 3 were moreresistant to microbial attack. Of the 10 strains, 6isolates were selected for detailed study. Theseisolates mineralized the isomers to CO2,NH4 +, and biomass. All strains weregram-negative rods with a strict aerobic metabolism. Characterization of physiological and biochemicalproperties revealed similarity between strains. Eeachstrain however, had a limited substrate range whichenabled it to degrade no more than 2 to 3 compounds ofthe 14 alkylpyridine isomers tested. Examination ofthe genetic variability among cultures with therandomly amplified polymorphic DNA technique revealedhigh levels of genomic DNA polymorphism. The highestsimilarity between 2 strains (0.653) was observedbetween 2-picoline and 3-picoline degrading cultures. The molecular basis of the differences in substratespecificity is under investigation.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 393-403 
    ISSN: 0886-1544
    Keywords: motility ; Ca2+ ; ionophores ; spirulina subsalsa ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Motility of the marine filamentous cyanobacterium Spirulina subsalsa is both Ca2+ and Na+ dependent, and replacement of Na+ by mannitol arrests it. The data presented suggest that Ca2+ interacts with sites on the surface of the cell membrane. The inhibitory effect of dicyclohexylcarbodiimide (DCCD) hints at the possibility that the role of Ca2+ may be associated with a membrane bound Ca-ATPase. Motility is pH dependent, being nil at pH 〈 6.5 and 〉 10.0, with an optimum at 8.5. Norepinephrine abolishes most of the inhibitory effect of low pH on motility. Ca2+ has an “all-or-none” effect on motility that is triggered at 5 mM. Acetylcholine lowers the threshold of Ca2+ necessary for triggering motility.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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