Publication Date:
2014-01-15
Description:
Ring chromosomes are structural aberrations commonly associated with birth defects, mental disabilities and growth retardation. Rings form after fusion of the long and short arms of a chromosome, and are sometimes associated with large terminal deletions. Owing to the severity of these large aberrations that can affect multiple contiguous genes, no possible therapeutic strategies for ring chromosome disorders have been proposed. During cell division, ring chromosomes can exhibit unstable behaviour leading to continuous production of aneuploid progeny with low viability and high cellular death rate. The overall consequences of this chromosomal instability have been largely unexplored in experimental model systems. Here we generated human induced pluripotent stem cells (iPSCs) from patient fibroblasts containing ring chromosomes with large deletions and found that reprogrammed cells lost the abnormal chromosome and duplicated the wild-type homologue through the compensatory uniparental disomy (UPD) mechanism. The karyotypically normal iPSCs with isodisomy for the corrected chromosome outgrew co-existing aneuploid populations, enabling rapid and efficient isolation of patient-derived iPSCs devoid of the original chromosomal aberration. Our results suggest a fundamentally different function for cellular reprogramming as a means of 'chromosome therapy' to reverse combined loss-of-function across many genes in cells with large-scale aberrations involving ring structures. In addition, our work provides an experimentally tractable human cellular system for studying mechanisms of chromosomal number control, which is of critical relevance to human development and disease.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030630/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030630/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bershteyn, Marina -- Hayashi, Yohei -- Desachy, Guillaume -- Hsiao, Edward C -- Sami, Salma -- Tsang, Kathryn M -- Weiss, Lauren A -- Kriegstein, Arnold R -- Yamanaka, Shinya -- Wynshaw-Boris, Anthony -- GM007085-32/GM/NIGMS NIH HHS/ -- K08 AR056299/AR/NIAMS NIH HHS/ -- RR18928/RR/NCRR NIH HHS/ -- U01 HL098179/HL/NHLBI NIH HHS/ -- U01HL098179/HL/NHLBI NIH HHS/ -- England -- Nature. 2014 Mar 6;507(7490):99-103. doi: 10.1038/nature12923. Epub 2014 Jan 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉1] Institute for Human Genetics and Department of Pediatrics, University of California, San Francisco, California 94143, USA [2] Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, California 94143, USA [3]. ; 1] Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA [2] Roddenberry Center for Stem Cell Biology and Medicine at Gladstone, San Francisco, California 94158, USA [3]. ; Department of Psychiatry, Institute for Human Genetics, University of California, San Francisco, California 94143, USA. ; Division of Endocrinology and Metabolism and Institute for Human Genetics, Department of Medicine, University of California, San Francisco, California 94143, USA. ; 1] Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA [2] Roddenberry Center for Stem Cell Biology and Medicine at Gladstone, San Francisco, California 94158, USA. ; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, California 94143, USA. ; 1] Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA [2] Roddenberry Center for Stem Cell Biology and Medicine at Gladstone, San Francisco, California 94158, USA [3] Department of Anatomy, University of California, San Francisco, San Francisco, California 94143, USA [4] Department of Reprogramming Science, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan. ; 1] Institute for Human Genetics and Department of Pediatrics, University of California, San Francisco, California 94143, USA [2] Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, Ohio 44106, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24413397" target="_blank"〉PubMed〈/a〉
Keywords:
Aneuploidy
;
Animals
;
Cellular Reprogramming/genetics
;
Chromosomal Instability/genetics
;
Chromosome Deletion
;
Chromosome Disorders/genetics/pathology
;
Chromosomes, Human, Pair 13/genetics
;
Chromosomes, Human, Pair 17/genetics
;
Clone Cells/cytology/metabolism
;
Fibroblasts/cytology/metabolism
;
Humans
;
Induced Pluripotent Stem Cells/*cytology/*metabolism
;
Karyotype
;
Karyotyping
;
Male
;
Mice
;
Models, Genetic
;
*Ring Chromosomes
;
Uniparental Disomy/genetics
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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