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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 314-320 
    ISSN: 1432-2242
    Keywords: Alcohol-soluble proteins ; Maize single mutants ; Maize double mutants ; Corn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Many maize (Zea mays L.) mutant genes exist. Some affect protein content or composition, while others modify carbohydrates or kernel phenotype. In doublemutant lines, two mutant genes are present. We know little about interactions of such genes, however. We therefore examined a normal maize inbred, B37, 10 near-isogenic single mutants and 46 double mutants to analyze quantitative effects on alcohol-soluble endosperm proteins. Proteins were extracted with 70% ethanol0.5% sodium acetate-5% mercaptoethanol, and fractionated by reversed-phase high-performance liquid chromatography (RP-HPLC). Early peaks were alcohol-soluble glutelin (ASG) subunits, while late peaks contained zein. Results were quantified and statistically analyzed. In many double mutants, protein compositions differed significantly from averages of compositions of corresponding single mutants. For example, a high-methionine, water-insoluble ASG is absent when the opaque-2 (o2) gene combines with shrunken-1 (sh1) or surgary-1 (su1). Another water-insoluble ASG nearly doubled when floury-2 (fl2) andsu1 combined. A high-proline, high-histidine, water-soluble ASG nearly doubled in combinations offl2 witho2,su1 and sugary-2 (su2). Zein was about half its expected value wheno2 combined with amylose-extender (ae), floury-1 (fl1), soft-starch (h),sh1 andsu1. Thus, rapid protein extraction and quantitative RP-HPLC showed major new epistatic and synergistic effects of several mutant genes on protein composition. Unexpectedly, these effects often involve genes that primarily affect starch composition or kernel phenotype. Alcohol-soluble proteins often vary in amount, as ino2 lines. They also differ in nutritional value. Thus, RP-HPLC analysis of these proteins can identify nutritionally superior genotypes, and may help explain the basis of such quality.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 85 (1992), S. 407-414 
    ISSN: 1432-2242
    Keywords: Maize ; Zein ; Prolamins ; Mutants Genes ; HPLC ; Quantitation ; Epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Zeins, the major endosperm proteins in maize (Zea mays L.), are deficient in the essential amino acids lysine and tryptophan. Some mutant genes, like opaque-2 (o2) and floury-2 (fl2), reduce the levels of A- and B-zeins, thereby improving maize's nutritional value. Other mutants, such as amylose-extender (ae), floury-1 (fl1), soft starch (h), dull-1 (du), shrunken-1 (sh1), sugary-1 (su1), sugary-2 (su2), and waxy (wx), primarily affect starch composition, but also alter zein composition. We undertook this study to examine the effects of some of these mutant genes on A/B-zein composition and to study the interactions of these genes in double-mutant combinations. Endosperm prolamins were extracted from inbred B37, ten near-isogenic single mutants (ae, du, fl1, fl2, h, o2, sh1, su1, su2, and wx), and most double-mutant combinations. Zeins in these extracts were fractionated by reversed-phase highperformance liquid chromatography (RP-HPLC) into 22–24 peaks. Of the resulting 22 major peaks the areas of 16 (per milligram endosperm) were significantly affected by individual mutant genes relative to the zein composition of the normal inbred. In combination these genes exhibited significant epistatic interactions in regulating the expression of individual A/B zeins. Epistatic interactions were judged to be significant when the amount of a peak in a double mutant differed from the averages for the peak in the two respective single mutants. The o2 gene, alone and in combination with other mutant genes, significantly decreased the amounts of many individual zeins. The effect of the o2 gene was the greatest of all the genes examined. Various clustering techniques were used to see if mutant effects could be grouped; among these was principal component analysis, a multivariate statistical technique that analyzes all peak sizes simultaneously. Three-dimensional scatter graphs were constructed based on the first three principal components. For the single mutants, these showed no relationships to gene actions; for the double mutants, however, this technique showed that four single mutants, o2, sh1, su1 and su2, had the greatest effects on zein composition when combined with each other and with the remaining six single mutants.
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  • 3
    ISSN: 1573-5036
    Keywords: Aflatoxin ; Aspergillus flavus ; Corn ; Fungi Maize ; Mycotoxins ; Naturally-occurring toxins ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Aflatoxin contamination of developing corn (Zea mays L.) kernels caused by natural infection byAspergillus flavus Link ex Fries was studied in hybrids developed for the U.S. corn belt and for the southern U.S. and grown at diverse locations in 1977. Planting dates were staggered to examine the effect of crop maturity on infection by the toxin-producing fungus. A broad range of toxin values was observed at harvest; some levels exceeded the highest that had been previously recorded in corn. The highest concentration of aflatoxin B1 detected was 8030 ppb. Levels of toxin differed significantly among planting dates in Florida and Georgia; the second planting date at these locations contained the highest toxin levels. Elevated concentrations of toxin were characteristic of kernel samples from southern locations and southeast Missouri; at these locations samples from hybrids developed for the south had significantly lower levels of toxin than hybrids developed for the corn belt. Ears with heavy insect damage had higher toxin levels than ears with less evidence of insect attack.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 48 (1990), S. 63-72 
    ISSN: 1573-5060
    Keywords: Zea mays ; maize ; corn ; yield ; kernel weight ; shelling percentage ; plant height ; ear height ; husk weight ; cob weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary White-endosperm (y y y) maize (Zea mays L.) is vital to the maize dry-milling industry. However, a substantially greater acreage in the U.S.A. is planted to yellow (Y Y Y) than white maize. Data on comparative performance of yellow and white maize is scanty, and results are inconclusive. This field study was conducted to provide information on dosage effects of y gene on grain yield, 500-kernel (K) weight, shelling percentage, plant and ear height, and cob weight. Yellow x yellow, yellow x white, and white x white endosperm crosses were made among five yellow and five white endosperm lines of Mo14W × Oh7B parentage. In the material studied, the genetic complement of the white-endosperm parent Mo14W varied from 12.50% to 87.50% across 15 treatments. The effect of Mo14W dosages among treatments was removed through analysis of covariance. If differences among treatment means existed, they were attributed to the y gene. A test of parallelism among regression coefficients (Ho. β1=β2=---β15=βc) indicated that these regressions coefficients for plant height, ear height, 500-K weight, and husk weight showed parallelism across yellow x yellow, yellow x white, and white x white crosses. Differences in plant height were significant in 3 out of 5 comparisons each between Y Y vs. y y, Y y vs. y y, and Y Y vs. Y y. Differences in ear height were significant in 1 of 5 Y Y vs. y y comparisons, in 3 of 5 Y y vs. y y comparisons, and in 1 of 5 Y Y vs. Y y comparisons. Differences in 500-K weight were significant in 4 of 5 Y Y vs. y y, in 2 of 5 Y Y vs. Y y and in 1 of 5 Y y vs. y y comparisons. Differences in husk weight were significant in all the Y Y vs. y y comparisons, in 4 of 5 Y y vs. y y comparisons, and in 1 of 5 Y Y vs. Y y comparisons. An increase in the y gene dosage caused, in general, a significant decrease in grain yield, 500-K weight, plant height, ear height, and husk weight. Shelling percentage was decreased but not appreciably. The Y y combination had a heterotic effect on plant height, ear height, and cob weight. An implication of the findings of this study is that it would be difficult to breed for high-yielding white maize if one used this y gene source. At this time, this is the only source (chromosome 6) available for white-endosperm maize. Non-parrallelism among regression coefficients was noted for shelling percentage, grain yield, and cob weight. Non-parallelism was observed in shelling percentage of the yellow x yellow crosses, cob weight of the yellow x white crosses and in grain yield of both yellow x yellow and yellow x white crosses. In general, an increase in mean Mo14W dosage resulted in increased grain yield. Mo14W dosages generally reduced cob weight in the yellow x white crosses. Shelling percentage increased as the mean Mo14W dosages increased for the yellow x yellow crosses. An increase in Mo14W dosages resulted in an increase in 500-K weight.
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  • 5
    Publication Date: 1969-05-01
    Print ISSN: 0002-1962
    Electronic ISSN: 1435-0645
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 6
    Publication Date: 1958-02-01
    Print ISSN: 0002-1962
    Electronic ISSN: 1435-0645
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 7
    Publication Date: 1988-01-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 8
    Publication Date: 1958-04-01
    Print ISSN: 0002-1962
    Electronic ISSN: 1435-0645
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 9
    Publication Date: 1971-09-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 10
    Publication Date: 1989-05-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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