Publikationsdatum:
2013-10-09
Beschreibung:
We describe a series of CEN/ARS episomal plasmids containing different Candida glabrata promoters, allowing for a range of constitutive or regulated expression of proteins in C. glabrata . The set of promoters includes three constitutive promoters ( EGD2 pr, HHT2 pr, PDC1 pr), two macrophage/phagocytosis-induced promoters ( ACO2 pr, LYS21 pr), and one nutritionally regulated promoter ( MET3 pr). Each promoter was cloned into two plasmid backbones that differ in their selectable marker, URA3 , or the dominant-selectable NAT1 gene, which confers resistance to the drug nourseothricin. Expression from the 12 resulting plasmids was assessed using GFP as a reporter and flow cytometry or quantitative reverse-transcription polymerase chain reaction to assess expression levels. Together this set of plasmids expands the toolkit of expression vectors available for use with C. glabrata .
Digitale ISSN:
2160-1836
Thema:
Biologie
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