ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
A novel porous composite scaffold of nano-HA/poly (lactic-co-glycolic) (PLGA) wasfabricated by solvent casting/particulate leaching method. Chondrocytes were isolated from theknee articular joints of a rabbit, and then seeded in the scaffolds. The cell-loaded scaffolds werecultured in vitro for 5 days before implantation. Full-thickness articular cartilage defects werecreated in rabbits, and filled with and without the cell-loaded nano-HA/PLGA scaffolds. Theimplants were harvested after in vivo incubation of 2 and 5 weeks. Cartilaginous tissues wereobserved at defects repaired with the cell-loaded scaffolds, while only fibrous tissues were foundfor the control groups. The repaired tissues were evaluated histologically by hematoxylin and eosinstaining. Results revealed that nano-HA/PLGA composite scaffolds facilitated adheration of cells invitro, and the nano-HA particles could prevented the scaffolds from collapsing and promoted theformation of cartilaginous tissue in vivo
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1185.pdf
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