ALBERT

Description: This study provides a comprehensive evaluation of the determinants of global Nmin with a focus on soil microbial biomass. The results demonstrated that soil microbial biomass predominantly controlled the variability of Nmin at a global scale. Climate, soil properties, and substrates influenced Nmin via their impacts on soil microbial biomass. The study highlights the importance of microbial biomass in determining soil nitrogen cycling, which challenges the conventional view that climate and soil properties are the dominant drivers of soil Nmin and advances our current understanding on the global patterns of nitrogen cycle. The findings suggest that changes in soil microbial biomass under global change would result in profound consequences on ecosystem processes by changing soil Nmin. Abstract Soil net nitrogen mineralization rate (Nmin), which is critical for soil nitrogen availability and plant growth, is thought to be primarily controlled by climate and soil physical and/or chemical properties. However, the role of microbes on regulating soil Nmin has not been evaluated on the global scale. By compiling 1565 observational data points of potential net Nmin from 198 published studies across terrestrial ecosystems, we found that Nmin significantly increased with soil microbial biomass, total nitrogen, and mean annual precipitation, but decreased with soil pH. The variation of Nmin was ascribed predominantly to soil microbial biomass on global and biome scales. Mean annual precipitation, soil pH, and total soil nitrogen significantly influenced Nmin through soil microbes. The structural equation models (SEM) showed that soil substrates were the main factors controlling Nmin when microbial biomass was excluded. Microbe became the primary driver when it was included in SEM analysis. SEM with soil microbial biomass improved the Nmin prediction by 19% in comparison with that devoid of soil microbial biomass. The changes in Nmin contributed the most to global soil NH4+‐N variations in contrast to climate and soil properties. This study reveals the complex interactions of climate, soil properties, and microbes on Nmin and highlights the importance of soil microbial biomass in determining Nmin and nitrogen availability across the globe. The findings necessitate accurate representation of microbes in Earth system models to better predict nitrogen cycle under global change.