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  • 1
    Electronic Resource
    Electronic Resource
    Structure and dispersion of iron-based catalysts for direct coal liquefaction (1993)
    s.l. : American Chemical Society
    Energy & fuels 7 (1993), S. 285-296 
    Details
    ISSN: 1520-5029
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ef00038a020
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Nondestructive determination of trace element speciation in coal and coal ash by XAFS spectroscopy (1993)
    s.l. : American Chemical Society
    Energy & fuels 7 (1993), S. 482-489 
    Details
    ISSN: 1520-5029
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ef00040a008
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Binary iron oxide catalysts for direct coal liquefaction (1994)
    s.l. : American Chemical Society
    Energy & fuels 8 (1994), S. 38-43 
    Details
    ISSN: 1520-5029
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ef00043a006
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Organic Acid Treatment of Ferrihydrite Catalyst for Improved Coal Liquefaction (1994)
    s.l. : American Chemical Society
    Energy & fuels 8 (1994), S. 1152-1153 
    Details
    ISSN: 1520-5029
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ef00047a021
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Arabidopsis thaliana tryptophan synthase alpha: Gene cloning, expression, and subunit interaction (1995)
    Springer
    Molecular genetics and genomics 248 (1995), S. 657-667 
    Details
    ISSN: 1617-4623
    Keywords: Tryptophan synthase ; trpA ; Subunit interaction ; Plant ; Complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The tryptophan synthaseα subunit catalyzes the conversion of indole-3-glycerolphosphate to indole, the penultimate reaction in the biosynthesis of the essential amino acid tryptophan. A cDNA encodingArabidopsis thaliana tryptophan synthaseα(TSA1) was isolated by complementation of anEscherichia coli ΔtrpA mutation and by polymerase chain reaction amplification from a cDNA library using degenerate primers. ATSA1 genomic clone was also isolated and 5 kb of the DNA sequence determined. A single sequence in the Arabidopsis genome with homology to theTSA1 cDNA was detected by high-stringency genomic Southern blot hybridization. In contrast under hybridization conditions of reduced stringency, one or two additional homologous sequences were observed. A 1.4 kb transcript was detected in wild-type RNA with theTSA1 cDNA as a probe. Several lines of evidence, including immunoaffinity chromatography, suggest that the activeA. thaliana tryptophan synthase enzyme consists of a heterosubunit complex, presumably analogous to the prokaryoticα 2 β 2 complex. Immunoblot analysis indicated that the plant α andβ subunits are present throughout development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02191705
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    Paper (German National Licenses)
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  • 6
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    Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2 (2015)
    PANGAEA
    In:  Supplement to: Wei, Lei; Wang, Qing; Ning, Xuanxuan; Mu, Changkao; Wang, Chunlin; Cao, Ruiwen; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin (2015): Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2. Comparative Biochemistry and Physiology Part D: Genomics & Proteomics, 13, 16-23, https://doi.org/10.1016/j.cbd.2014.12.001
    Details
    Publication Date: 2024-03-15
    Description: Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); Crassostrea gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Laboratory experiment; Mollusca; mRNA gene expression, relative; mRNA gene expression, relative, standard deviation; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Salinity; Salinity, standard deviation; Single species; South Pacific; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 540 data points
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    DATA PANGAEA
  • 7
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    Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO2 exposure (2015)
    PANGAEA
    In:  Supplement to: Wei, Lei; Wang, Qing; Wu, Huifeng; Ji, Chenglong; Zhao, Jianmin (2014): Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO2 exposure. Journal of Proteomics, 112, 83-94, https://doi.org/10.1016/j.jprot.2014.08.010
    Details
    Publication Date: 2024-03-15
    Description: The gradually increased atmospheric CO2 partial pressure (pCO2) has thrown the carbonate chemistry off balance and resulted in decreased seawater pH in marine ecosystem, termed ocean acidification (OA). Anthropogenic OA is postulated to affect the physiology of many marine calcifying organisms. However, the susceptibility and metabolic pathways of change in most calcifying animals are still far from being well understood. In this work, the effects of exposure to elevated pCO2 were characterized in gills and hepatopancreas of Crassostrea gigas using integrated proteomic and metabolomic approaches. Metabolic responses indicated that high CO2 exposure mainly caused disturbances in energy metabolism and osmotic regulation marked by differentially altered ATP, glucose, glycogen, amino acids and organic osmolytes in oysters, and the depletions of ATP in gills and the accumulations of ATP, glucose and glycogen in hepatopancreas accounted for the difference in energy distribution between these two tissues. Proteomic responses suggested that OA could not only affect energy and primary metabolisms, stress responses and calcium homeostasis in both tissues, but also influence the nucleotide metabolism in gills and cytoskeleton structure in hepatopancreas. This study demonstrated that the combination of proteomics and metabolomics could provide an insightful view into the effects of OA on oyster C. gigas. BIOLOGICAL SIGNIFICANCE: The gradually increased atmospheric CO2 partial pressure (pCO2) has thrown the carbonate chemistry off balance and resulted in decreased seawater pH in marine ecosystem, termed ocean acidification (OA). Anthropogenic OA is postulated to affect the physiology of many marine calcifying organisms. However, the susceptibility and metabolic pathways of change in most calcifying animals are still far from being understood. To our knowledge, few studies have focused on the responses induced by pCO2 at both protein and metabolite levels. The pacific oyster C. gigas, widely distributed throughout most of the world's oceans, is a model organism for marine environmental science. In the present study, an integrated metabolomic and proteomic approach was used to elucidate the effects of ocean acidification on Pacific oyster C. gigas, hopefully shedding light on the physiological responses of marine mollusk to the OA stress.
    Keywords: Alkalinity, total; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); Coulometric titration; Crassostrea gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Laboratory experiment; Mollusca; mRNA gene expression, relative; mRNA gene expression, relative, standard deviation; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Potentiometric; Protein name; Salinity; Single species; Species; Temperate; Temperature, water; Tissues; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 352 data points
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    DATA PANGAEA
  • 8
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    Seawater carbonate chemistry and immune function of the Pacific oyster against Vibrio splendidus challenge (2018)
    PANGAEA
    In:  Supplement to: Cao, Ruiwen; Wang, Qing; Yang, Dinglong; Liu, Yongliang; Ran, Wen; Qu, Yi; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin (2018): CO 2 -induced ocean acidification impairs the immune function of the Pacific oyster against Vibrio splendidus challenge: An integrated study from a cellular and proteomic perspective. Science of the Total Environment, 625, 1574-1583, https://doi.org/10.1016/j.scitotenv.2018.01.056
    Details
    Publication Date: 2024-03-15
    Description: Ocean acidification (OA) and pathogenic diseases pose a considerable threat to key species of marine ecosystem. However, few studies have investigated the combined impact of reduced seawater pH and pathogen challenge on the immune responses of marine invertebrates. In this study, Pacific oysters, Crassostrea gigas, were exposed to OA (~2000 ppm) for 28 days and then challenged with Vibrio splendidus for another 72 h. Hemocyte parameters showed that V. splendidus infection exacerbated the impaired oyster immune responses under OA exposure. An iTRAQ-based quantitative proteomic analysis revealed that C. gigas responded differently to OA stress and V. splendidus challenge, alone or in combination. Generally, OA appears to act via a generalized stress response by causing oxidative stress, which could lead to cellular injury and cause disruption to the cytoskeleton, protein turnover, immune responses and energy metabolism. V. splendidus challenge in oysters could suppress the immune system directly and lead to a disturbed cytoskeleton structure, increased protein turnover and energy metabolism suppression, without causing oxidative stress. The combined OA- and V. splendidus-treated oysters ultimately presented a similar, but stronger proteomic response pattern compared with OA treatment alone. Overall, the impaired oyster immune functions caused by OA exposure may have increased the risk of V. splendidus infection. These results have important implications for the impact of OA on disease outbreaks in marine invertebrates, which would have significant economic and ecological repercussions.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); Crassostrea gigas; Experiment duration; Fold change; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression; Gene expression, standard deviation; Group; Hemocyte count; Hemocyte count, standard deviation; Immunology/Self-protection; Laboratory experiment; Mollusca; Name; North Pacific; Number of expressed proteins; OA-ICC; Ocean Acidification International Coordination Centre; Other; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Percentage; Percentage, standard deviation; pH; pH, standard deviation; Phagocytosis rate; Phagocytosis rate, standard deviation; Potentiometric; Potentiometric titration; Reactive oxygen species production; Reactive oxygen species production, standard deviation; Registration number of species; Salinity; Salinity, standard deviation; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 1458 data points
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    DATA PANGAEA
  • 9
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    Seawater carbonate chemistry and Cu accumulation and cellular toxicity in the Pacific Oyster Crassostrea gigas (2019)
    PANGAEA
    Details
    Publication Date: 2024-03-20
    Description: Ocean acidification (OA) has been found to increase the release of free Cu2+ in seawater. However, only a handful of studies have investigated the influence of OA on Cu accumulation and cellular toxicity in bivalve species. In this study, Pacific oysters, Crassostrea gigas, were exposed to 25 μg/L Cu2+ at three pH levels (8.1, 7.8 and 7.6) for 14 and 28 days. Physiological and histopathological parameters [(clearance rate (CR), respiration rate (RR), histopathological damage and condition index (CI)), oxidative stress and neurotoxicity biomarkers [superoxide dismutase (SOD) and glutathione transferase (GST) activities, lipid peroxidation (LPO) and acetylcholinesterase (AChE) activity], combined with glycolytic enzyme activities [pyruvate kinase (PK) and hexokinase (HK)] were investigated in C. gigas. The bioconcentration of Cu was increased in soft tissues of Cu-exposed oysters under OA. Our results suggest that both OA and Cu could lead to physiological disturbance, oxidative stress, cellular damage, disturbance in energy metabolism and neurotoxicity in oysters. The inhibited CR, increased glycolytic enzymes activities and decreased CI suggested that the energy metabolism strategy adopted by oysters was not sustainable in the long term. Furthermore, integrated biomarker response (IBR) results found that OA and Cu exposure lead to severe stress to oysters, and co-exposure was the most stressful condition. Results from this study highlight the need to include OA in future environmental assessments of pollutants and hazardous materials to better elucidate the risks of those environmental perturbations.
    Keywords: Acetylcholinesterase activity, standard deviation; Acetylcholinesterase activity, unit per protein mass; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Behaviour; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Clearance rate; Clearance rate, standard deviation; Coast and continental shelf; Condition index; Condition index, standard deviation; Containers and aquaria (20-1000 L or 〈 1 m**2); Copper; Copper, standard deviation; Crassostrea gigas; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Glutathione S-transferase activity, standard deviation; Glutathione S-transferase activity, unit per protein mass; Hexokinase activity, per protein mass; Hexokinase activity, standard deviation; Inorganic toxins; Integrated biomarker response index; Laboratory experiment; Lipid peroxidation, per protein; Lipid peroxidation, standard deviation; Mollusca; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Potentiometric; Potentiometric titration; Pyruvate kinase activity, per protein; Pyruvate kinase activity, standard deviation; Replicates; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard deviation; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Superoxide dismutase activity, standard deviation; Superoxide dismutase activity, unit per protein mass; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type
    Type: Dataset
    Format: text/tab-separated-values, 732 data points
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    DATA PANGAEA
  • 10
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    Bearing fault detection in varying operational conditions using wavelet packet decomposition and support vector machine (2018)
    Institute of Physics
    Details
    Publication Date: 2018-09-01
    Print ISSN: 1742-6588
    Electronic ISSN: 1742-6596
    Topics: Physics
    Published by Institute of Physics
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    PAPER CURRENT
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