ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The K1 killer toxin of Saccharomyces cerevisiae consists of 103- and 83-residue α and β components whose derivation, from a 316-residue precursor preprotoxin, requires processing at the αN-terminus (after ProArg-44), the αC-terminus (after ArgArg-149) and at the βN-terminus (after LysArg–233). These processing events occur after translocation to the Golgi and have been investigated using β-lactamase fusions. Signal peptidase cleavage of the precursor, predicted to occur after Ala-26, was confirmed by N-terminal sequence analysis of Ala-34 and IIe-52 fusions. Cleavage at all of the other predicted processing sites, including ProArg-44, is dependent on activity of the Kex2 protease. A fourth Kex2-dependent cleavage occurs at LysArg-188. Implications for the specificity of Kex2 cleavage and preprotoxin processing are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The widespread distribution of the tumor suppressor PTEN (phosphatase and tensin homolog deleted on chromosome 10) in the adult brain suggests its role in a broad range of brain functions. Here we show evidence supporting a physical interaction of PTEN with a region in the third intracellular loop ...
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 207 (2000), S. 3-8 
    ISSN: 1573-4919
    Keywords: phospholipase D ; human hepatocarcinoma cell line ; adenine guanine nucleotides ; protein kinase A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The regulation of phosphatidylcholine-specific phospholipase D by purine nucleotides and protein kinase A were studied in vitro using an enzyme preparation partially purified from the membranous fraction of 7721 hepatocarcinoma cells. It was found that the enzyme activity was elevated by low concentrations of some purine nucleotides, but the activating effects were decreased when the concentrations of the nucleotides were higher. The optimal concentrations of GTP, GTPγ[S] , GDP and ATP for maximal activation were 0.1mM, 5μM,1 mM and 1 mM respectively. The activation caused by 1mM ADP was lower. The enzyme was not activated by 1mM AMP, but significant activation was observed by the addition of 1mM cAMP. The latter was mediated by protein kinase A, as a specific inhibitor of protein kinase A ablished the activation. There were synergic effects between ATP and GTP, ATP and PIP2, but not between ATP and GTPγ[S] , or PIP2 and GTPγ[S]. The activating effects of GTP and ATP were abolished by neomycin, a PIP2 scavenger. These results suggest that phospholipase D is regulated by GTP-binding protein and the presence of PIP2 is required for the activation induced by GTP. Protein kinase A may be another protein kinase in addition to protein kinase C and protein tyrosine kinase which regulate the activity of phospholipase D, when the intracellular concentration of cAMP is increased.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Environment, development and sustainability 2 (2000), S. 333-345 
    ISSN: 1573-2975
    Keywords: China ; Yunnan Province ; biodiversity ; cultural diversity ; geographic information systems ; ecological economics ; economic development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Sociology
    Notes: Abstract Chinese and American scientists are co-operating to develop concepts, strategies, agreements, and proposals in support of an economic development and sustainable ecosystems project in Yunnan Province, People's Republic of China. Yunnan's Provincial Government has initiated a major programme to develop and further utilise its biological resources to help improve economic conditions for its citizens. They are co-operating with the US Geological Survey (USGS) on evaluation and management of biological resources so economic development will be compatible with sustainable ecological systems. Scientists from the USGS and co-operating universities will provide expertise on synthesising biological data, conducting a Gap Analysis for the Province, evaluating innovative economic opportunities, and designing an effective education, training, and outreach programme.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 9 (1993), S. 251-266 
    ISSN: 0749-503X
    Keywords: Secretion ; virus-like particle ; double-stranded RNA ; Saccharomyces cerevisiae ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: K1 killer strains of Saccharomyces cerevisiae secrete a polypeptide toxin to which they are themselves immune. The α and β components of toxin comprise residues 45-147 and 234-316 of the 316-residue K1 preprotoxin. The intervening 86-residue segment is called γ. A 26-residue signal peptide is removed on entry into the endoplasmic reticulum. The Kex2 protease excises the toxin components from the 290-residue glycosylated protoxin in a late Golgi compartment. Expression of a cDNA copy of the preprotoxin gene confers the complete K1 killer phenotype on sensitive cells. We now show that expression of immunity requires that α component and the N-terminal 31 residues of γ. An additional C-terminal extension, either eight residues of γ or three of four unrelated peptides, is also required. Expression of preprotoxin terminating at the α C-terminus, or lacking the γ N-terminal half of γ causes profound but reversible growth inhibition. Inhibition is suppressed in cis by the same 31 residues of γ required for immunity to exocellular toxin in trans, but not by the presence of β. Both immunity and growth inhibition are alleviated by insertions in α that inactivate toxin. Inhibition is not suppressed by kex2, chc1 or kre1 mutations, by growth at higher pH or temperature, or by normal K1 immunity. Inhibition, therefore, probably does not involve processing of the α toxin component at its N-terminus or release from the cell and binding to glucan receptors. Some insertion and substitution mutations in γ severely reduce toxin secretion without affecting immunity. They are presumed to affect protoxin folding in the endoplasmic reticulum and translocation to the Golgi.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    Publication Date: 2022-05-26
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lin, H., Tarnas, J. D., Mustard, J. F., Zhang, X., Wei, Y., Wan, W., Klein, F., & Kellner, J. R. Dynamic aperture factor analysis/target transformation (DAFA/TT) for Mg-serpentine and Mg-carbonate mapping on Mars with CRISM near-infrared data. Icarus, 355, (2021): 114168, https://doi.org/10.1016/j.icarus.2020.114168.
    Description: Serpentine and carbonate are products of serpentinization and carbonation processes on Earth, Mars, and other celestial bodies. Their presence implies that localized habitable environments may have existed on ancient Mars. Factor Analysis and Target Transformation (FATT) techniques have been applied to hyperspectral data from the Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) to identify possible serpentine and Mg-carbonate-bearing outcrops. FATT techniques are capable of suggesting the presence of individual spectral signals in complex spectral mixtures. Applications of FATT techniques to CRISM data thus far only evaluate whether an entire analyzed image (≈ 3 × 105 pixels) may contain spectral information consistent with a specific mineral of interest. The spatial distribution of spectral signal from the possible mineral is not determined, making it difficult to validate a reported detection and also to understand the geologic context of any purported detections. We developed a method called Dynamic Aperture Factor Analysis/Target Transformation (DAFA/TT) to highlight the locations in a CRISM observation (or any similar laboratory or remotely acquired data set) most likely to contain spectra of specific minerals of interest. DAFA/TT determines the locations of possible target mineral spectral signals within hyperspectral images by performing FATT in small moving windows with different geometries, and only accepting pixels with positive detections in all cluster geometries as possible detections. DAFA/TT was applied to a hyperspectral image of a serpentinite from Oman for validation testing in a simplified laboratory setting. The mineral distribution determined by DAFA/TT application to the laboratory hyperspectral image was consistent with Raman analysis of the serpentinite sample. DAFA/TT also successfully mapped the spatial distribution of Mg-serpentine and Mg-carbonate previously detected in CRISM data using band parameter mapping and extraction of ratioed spectra. We applied DAFA/TT to CRISM images in some olivine-rich regions of Mars to characterize the spatial distribution of Mg-serpentine and Mg-carbonate-bearing outcrops.
    Description: This work was supported by the National Natural Science Foundation of China (grant no. 41671360, 41525016, 41902318). JFM and JDT acknowledge NASA support through a subcontract from the Applied Physics Lab for CRISM investigations. H. Lin also acknowledges the support from the key research Program of the Institute of Geology and Geophysics, CAS (IGGCAS-201905). The Headwall imaging spectrometer was acquired using funds to JRK from The Institute at Brown for Environment and Society and Brown University. The DAFA/TT codes are available on GitHub (https://github.com/linhoml?tab=repositories).
    Keywords: Dynamic aperture ; Factor analysis and target transformation ; Serpentine ; Carbonate ; Mars
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    Publication Date: 2019-09-23
    Description: sugary enhancer1 (se1) is a naturally occurring mutant allele involved in starch metabolism in maize endosperm. It is a recessive modifier of sugary1 (su1) and commercially important in modern sweet corn breeding, but its molecular identity and mode of action remain unknown. Here, we developed a pair of near-isogenic lines, W822Gse (su1-ref/su1-ref se1/se1) and W822GSe (su1-ref/su1-ref Se1/Se1), that Mendelize the se1 phenotype in an su1-ref background. W822Gse kernels have lower starch and higher water soluble polysaccharide and sugars than W822GSe kernels. Using high-resolution genetic mapping, we found that wild-type Se1 is a gene Zm00001d007657 on chromosome 2 and a deletion of this gene causes the se1 phenotype. Comparative metabolic profiling of seed tissue between these 2 isolines revealed the remarkable difference in carbohydrate metabolism, with sucrose and maltose highly accumulated in the mutant. Se1 is predominantly expressed in the endosperm, with low expression in leaf and root tissues. Differential expression analysis identified genes enriched in both starch biosynthesis and degradation processes, indicating a pleiotropic regulatory effect of se1. Repressed expression of Se1 and Su1 in RNA interference-mediated transgenic maize validates that deletion of the gene identified as Se1 is a true causal gene responsible for the se1 phenotype. The findings contribute to our understanding of starch metabolism in cereal crops.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    Publication Date: 2018-05-07
    Description: Expression of HMGA2 is strongly associated with body size and growth in mice and humans. In mice, inactivation of one or both alleles of Hmga2 results in body-size reductions of 20% and 60%, respectively. In humans, microdeletions involving the HMGA2 locus result in short stature, suggesting the function of the HMGA2 protein is conserved among mammals. To test this hypothesis, we generated HMGA2-deficient pigs via gene editing and somatic cell nuclear transfer (SCNT). Examination of growth parameters revealed that HMGA2−/+ male and female pigs were on average 20% lighter and smaller than HMGA2+/+ matched controls (P 〈 0.05). HMGA2−/− boars showed significant size reduction ranging from 35 to 85% of controls depending on age (P 〈 0.05), and organ weights were also affected (P 〈 0.05). HMGA2−/+ gilts and boars exhibited normal reproductive development and fertility, while HMGA2−/− boars were sterile due to undescended testes (cryptorchidism). Crossbreeding HMGA2−/+ boars and gilts produced litters lacking the HMGA2−/− genotype. However, analysis of day (D) D40 and D78 pregnancies indicated that HMGA2−/− fetuses were present at the expected Mendelian ratio, but placental abnormalities were seen in the D78 HMGA2−/− concepti. Additionally, HMGA2−/− embryos generated by gene editing and SCNT produced multiple pregnancies and viable offspring, indicating that lack of HMGA2 is not lethal per se. Overall, our results show that the effect of HMGA2 with respect to growth regulation is highly conserved among mammals and opens up the possibility of regulating body and organ size in a variety of mammalian species including food and companion animals.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Publication Date: 2004-06-07
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    Publication Date: 2010-11-01
    Print ISSN: 0021-8979
    Electronic ISSN: 1089-7550
    Topics: Physics
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...