Publication Date:
2004-11-16
Description:
Objective: The experiment is to evaluate the effect of FL, SCF, TPO on CD34+ expansion. Materials and methods: Cord blood samples were collected in heparinized tubes by the Obstetrics and Gynecology Department of Guangzhou first Municipal Peoples hospital. Mononuclear cells(MNCs) were isolated by Ficoll gradient separation. MNCs were culture in 50ml flask containing 6ml serum-free liquid culture system for 14 days at a density of 1*106/ml according to different cytokines combinations. A: control group, no cytokines were added in the culture system. B: cells cultured in the group of SCF+FL+TPO+EPO+IGF-1. C: cells cultured in the group of SCF+FL+TPO. The final concentration of cytokines was 10ng/ml for TPO, 25ng/ml for FL, 25ng/ml for SCF, respectively. Replacing half of media containing the same concentration cytokines on day 6, renewal of Group B and C at day 6, 10 and 14 for final concentration 5U/L EPO, 50ng/ml IGF-1. Part of the cells on day 6, 10 and 14 were counted and detected erythroid progenitors and CFU-GM on semi-solid culture system and the proportion of CD34+, CD34+ CD71+, CD71+ GPA+ cells was detected by FACS. All calculation was performed using SPSS program. Results:1. Proliferation of the total cells: After 10 days, the total cord blood cells were increased 6.89 folds in group B and 3.06 folds in group C respectively. The latter two groups had highly significant differences with the control group A(p
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
Permalink