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  • 1
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: The structural framework of soil mediates all soil processes, at all relevant scales. The spatio-temporal heterogeneity prevalent in most soils underpins the majority of biological diversity in soil, providing refuge sites for prey against predator, flow paths for biota to move, or be moved, and localized pools of substrate for biota to multiply. Just as importantly, soil biota play a crucial role in mediating soil structure: bacteria and fungi aggregate and stabilize structure at small scales (μm–cm) and earthworms and termites stabilize and create larger-scale structures (mm–m). The stability of this two-way interaction of structure and biota relations is crucial to the sustainability of the ecosystem.Soil is constantly reacting to changes in microclimates, and many of the soil–plant–microbe processes rely on the functioning of subtle chemical and physical gradients. The effect of global change on soil structure–biota interactions may be significant, through alterations in precipitation, temperature events, or land-use. Nonetheless, because of the complexity and the ubiquitous heterogeneity of these interactions, it is difficult to extrapolate from general qualitative predictions of the effects of perturbations to specific reactions. This paper reviews some of the main soil structure–biota interactions, particularly focusing on soil stability, and the role of biota mediating soil structures. The effect of alterations in climate and land-use on these interactions is investigated. Several case studies of the effect of land-use change are presented.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 44 (2003), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The mycelial growth form of eucarpic fungi allows for a highly effective spatial exploration of the soil habitat. However, understanding mycelial spread through soil has been limited by difficulties of observation and quantification of fungi as they spread through this matrix. We report on a study on the effects of soil structure by altering the soil bulk density, on the spatial exploration of soil by the fungus Rhizoctonia solani using a soil thin-sectioning technique. First we quantified fungal densities in microscopic images (0.44 mm2). At this scale, hyphae were either absent, or present as minor fragments, typically occupying less than 1% surface area of the thin section. From contiguous microscopic images we then produced large-scale (6.21 cm2) spatial distribution maps of fungal hyphae. These maps were superimposed onto soil structural maps, which quantify the degree of porosity in each microscopic image. Alterations in soil structure by changing the bulk density are shown to affect the distribution of the fungus within the soil. The volume of soil explored by the fungus increased with increasing bulk density. This was associated with a shift from a few large pore spaces to more evenly distributed small-scale pores. Fungal hyphae were present in all porosity classes within each bulk density, including areas that contain less than 5% visible pore space. However, fungal hyphae were more often found in areas with a higher porosity, in particular at low soil bulk densities. The results show that soil structure is a major component in the spatial exploration of soil by fungi.
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  • 3
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We investigated the influence of root border cells on the colonisation of seedling Zea mays roots by Pseudomonas fluorescens SBW25 in sandy loam soil packed at two dry bulk densities. Numbers of colony forming units (CFU) were counted on sequential sections of root for intact and decapped inoculated roots grown in loose (1.0 mg m−3) and compacted (1.3 mg m−3) soil. After two days of root growth, the numbers of P. fluorescens (CFU cm−1) were highest on the section of root just below the seed with progressively fewer bacteria near the tip, irrespective of density. The decapped roots had significantly more colonies of P. fluorescens at the tip compared with the intact roots: approximately 100-fold more in the loose and 30-fold more in the compact soil. In addition, confocal images of the root tips grown in agar showed that P. fluorescens could only be detected on the tips of the decapped roots. These results indicated that border cells, and their associated mucilage, prevented complete colonization of the root tip by the biocontrol agent P. fluorescens, possibly by acting as a disposable surface or sheath around the cap.
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  • 4
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The density and spatial location of bacteria were investigated within different horizons of an upland grassland soil before and after a liming treatment to increase the numbers of large soil fauna. Bacterial cells were located by image analysis of stained thin sections and densities calculated from these data. Excrement from macro- and meso-fauna was identified using micromorphology and the densities of bacteria on specific areas of excrement measured by image analysis. There were significant differences among horizons in the density of bacterial cells, with the minimum density found in the horizon with least evidence of earthworm activity, but no difference in density between the organic H and organo-mineral Ah horizons. Soil improvement by liming significantly increased bacterial densities in all three horizons, with the greatest increase found in the horizon with the smallest density before liming.There were no differences in bacterial density between areas dominated by excrement from earthworms and excrement from enchytraeids, although densities in both areas were significantly increased by liming. Variability in bacterial density at spatial scales of less than 1 mm was linked to the occurrence of excrement. Bacterial densities within areas of both types of excrement were significantly greater than those in the surrounding soil. However, the frequency distribution of the ratios of density in excrement to that in the soil was bimodal, with a majority of occurrences having a ratio near 1 and only some 20–30% having a much larger ratio. These variations can probably be explained by variations in the age of the excrement and its suitability as a substrate.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 44 (2003), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Biological soil thin-sections and a combination of image analysis and geostatistical tools were used to conduct a detailed investigation into the distribution of bacteria in soil and their relationship with pores. The presence of spatial patterns in the distribution of bacteria was demonstrated at the microscale, with ranges of spatial autocorrelation of 1 mm and below. Bacterial density gradients were found within bacterial patches in topsoil samples and also in one subsoil sample. Bacterial density patches displayed a mosaic of high and low values in the remaining subsoil samples. Anisotropy was detected in the spatial structure of pores, but was not detected in relation to the distribution of bacteria. No marked trend as a function of distance to the nearest pore was observed in bacterial density values in the topsoil, but in the subsoil bacterial density was greatest close to pores and decreased thereafter. Bacterial aggregation was greatest in the cropped topsoil, though no consistent trends were found in the degree of bacterial aggregation as a function of distance to the nearest pore. The implications of the results presented for modelling and predicting bacterial activity in soil are discussed.
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  • 6
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A method for determining the number and in situ spatial distribution of bacterial cells over spatial scales ranging from micrometres to centimetres in mineral soils is described. Biological thin sections of undisturbed cores of soil were prepared in order to preserve the spatial distribution of bacterial cells. Composite (tessellated) images in which individual bacteria can be resolved within an area of 0.282 mm2 were acquired by means of a motorised scanning microscope stage. An image processing and analysis procedure was developed to determine the numbers and locations of bacterial cells in the composite images. The image processing procedure first homogenised the background of the images and then discriminated between bacteria and non-bacterial features using the colour and morphological properties of the images of the bacterial cells. Feature edges were detected in the green channel of colour (red, green, blue) images and bacterial cell edges were confirmed in the blue channel after elimination of autofluorescent features in the red channel. No significant difference was found between the number of bacteria or associated distributions determined automatically and control values derived interactively on individual fields of view. Data relating to total bacterial counts in thin sections and in paired dispersed samples suggested that all soil bacteria were being visualised in thin sections. Significant differences between samples taken from a depth profile of a fallow arable soil were found for both cell numbers and for cell distribution as measured by an index of dispersion.
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  • 7
    ISSN: 1573-5036
    Keywords: maize ; nitrate ; nitrogen fertilizer ; root zone ; soil solution ; soil solution sampler with looped hollow fiber (LHF-sampler) ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of inorganic nitrogen (N) fertilizer on the ionic composition of the soil solution under maize (Zea mays L.) was studied. A pot experiment was carried out with two treatments combined factorially, with or without N application (Ca(NO3)2; +N and −N treatments, respectively), and with or without plants. Three looped hollow fiber samplers were installed in each pot to sample soil solutions nondestructively from the root zone, seven times during the 50-day growth period. Plants were harvested on the 50th day, and their nutrient contents determined. Effects of N fertilizer on the soil solutions were observed by the first sampling, 2 days after sowing. The concentrations of Ca and NO3 − and electrical conductivity (EC) increased significantly in the +N treatments as direct effects of fertilizer application. In addition, the concentrations of Mg, K, Na and H+ also increased and that of P decreased significantly as indirect effects caused by the re-establishment of chemical equilibria. This suggested the greater supply as well as the greater possibility of leaching loss not only of NO3 − but also of Ca, Mg and K. In the treatments with plants, the concentrations of NO3 −, Ca, Mg and K decreased with time and pH increased significantly compared with the unplanted soil. The depletion of N in the soil solution roughly agreed with the amount of N taken up by the plant. The depletions of K from the soil solution amounted to less than 10% of the amount of the K taken up, suggesting intensive replenishment of K from exchange sites in the soil. Depletions of Ca and Mg were several times higher than the amounts taken up, indicating that the depletions resulted from the adsorption of the divalent cations by the soil rather than uptake by plants. Because NO3 − is hardly absorbed by exchange sites in soil and was the dominant anion in solution, it was concluded that NO3 − had a major role in controlling cation concentrations in the soil solution and, consequently, on their availability for uptake by plants as well as their possible leaching loss. ei]H Marschner
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  • 8
    ISSN: 1573-5036
    Keywords: leaching potential ; mass flow ; nitrate ; root zone ; soil solution ; wheat ( Triticum aestivum L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Adding nitrogen (N) fertilizers to soil affects not only the concentration in the soil solution of the added ions, but also those of other ions already present in the soil. This secondary effect is caused by ion exchange and electrochemical equilibrium processes. We studied how different N fertilizers affected the chemical composition of the soil solution over time, and how this related to nutrient uptake by wheat. Soil was fertilized either with (NH4)2SO4 or Ca(NO3)2, or no N was added. Each of these N treatments was either planted or not with spring wheat (Triticum aestivum L.). Soil solutions were collected repeatedly with looped hollow fiber samplers from the root zone in situ, six times during a 50-day pot experiment. Plants were harvested five times, and their nutrient contents determined. In the soil solution, NO3- was significantly less concentrated if (NH4)2SO4, rather than Ca(NO3)2 was applied, until after net nitrification had ended on day 20. In contrast, Ca2+, Mg2+ and K+ were significantly more concentrated in the former treatment. This was probably caused by the greater concentration of anions that resulted from nitrification. P was always very dilute and unaffected by the form of N fertilizer. The form of N fertilizer had no significant effect on plant growth and nutrient uptake. The likely contribution of mass flow of the soil solution in supplying Ca, Mg and N to the plants was greatest when (NH4)2SO4 was supplied. The supply of K and P was unaffected by N fertilizer. The potential for N leaching loss was lower with (NH4)2SO4 than with Ca(NO3)2, especially up to day 20. However, the potential for cations leaching loss was greater in the (NH4)2SO4 treatment. This suggests that there is only a limited advantage in fertilizing with (NH4)2SO4 to reduce the total loss of nutrients from soil.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 14 (1979), S. 3008-3010 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 14 (1979), S. 1579-1585 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This work constitutes a closer investigation of part of the system, PbO/TeO2, with particular attention to the existence and nature of a 1∶1 molar ratio compound, following inconsistencies in previous publications. Observations on previous attempts to pull crystals from the melt are reconsidered in the light of the current work.
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