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  • 1
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    Beam test study of the MRPC-based T0 detector for the CEE (2019)
    Institute of Physics
    Details
    Publication Date: 2019-09-20
    Electronic ISSN: 1748-0221
    Topics: Physics
    Published by Institute of Physics
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    PAPER CURRENT
  • 2
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    Early development of undulated surf clam, Paphia undulate under elevated pCO2 (2016)
    PANGAEA
    In:  Supplement to: Guo, X; Xu, Xiaowei; Zhang, Pengfei; Huang, M; Luo, Xuan; You, W; Ke, C (2016): Early development of undulated surf clam, Paphia undulate under elevated pCO2. Journal of Experimental Marine Biology and Ecology, 484, 23-30, https://doi.org/10.1016/j.jembe.2016.08.002
    Details
    Publication Date: 2024-03-15
    Description: Increasing atmospheric CO2 can decrease the seawater pH and carbonate ions, which may adversely affect the larval survival of calcareous animals. In this study, we simulated future atmospheric CO2 concentrations (800, 1500, 2000 and 3000 µatm) and examined the effects of ocean acidification on the embryonic and larval stage of an infaunal clam Paphia undulate. Significant decrease of hatching of P. undulate was observed when the pCO2 reached 3000 µatm, and larval deformation rate increased significantly when pCO2 reached 2000 µatm, indicating a strong tolerance to ocean acidification compared with the embryonic development of other bivalves. The larvae cultured in 1500 µatm pCO2 exhibited the fastest growth, highest survival and shortened planktonic period, which unordinary phenomenon reflected the beneficial effect of ocean acidification on P. undulate larval development. The better development of P. undulate larvae under a higher CO2 condition maybe an adaptation in response to the acidified sediment in which they live.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Development; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Hatching rate; Incubation duration; Laboratory experiment; Larval deformity rate; Metamorphosis rate; Mollusca; Mortality/Survival; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Paphia undulate; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Potentiometric; Potentiometric titration; Registration number of species; Replicate; Reproduction; Salinity; Salinity, standard deviation; Shell increment; Shell length; Single species; Species; Survival; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference; Zooplankton
    Type: Dataset
    Format: text/tab-separated-values, 5667 data points
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    DATA PANGAEA
  • 3
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    Effects of ocean acidification caused by rising CO2 on the early development of three mollusks (2015)
    PANGAEA
    In:  Supplement to: Guo, X; Huang, M; Pu, F; You, W; Ke, C (2015): Effects of ocean acidification caused by rising CO2 on the early development of three mollusks. Aquatic Biology, 23(2), 147-157, https://doi.org/10.3354/ab00615
    Details
    Publication Date: 2024-03-15
    Description: Increasing atmospheric CO2 can decrease seawater pH and carbonate ions, which may adversely affect the larval survival of calcareous animals. In this study, we simulated future atmospheric CO2 concentrations (800, 1500, 2000 and 3000 ppm) and examined the effects of ocean acidification on the early development of 3 mollusks (the abalones Haliotis diversicolor and H. discus hannai and the oyster Crassostrea angulata). We showed that fertilization rate, hatching rate, larval shell length, trochophore development, veliger survival and metamorphosis all decreased significantly at different pCO2 levels (except oyster hatching). H. discus hannai were more tolerant of high CO2 compared to H. diversicolor. At 2000 ppm CO2, 79.2% of H. discus hannai veliger larvae developed normally, but only 13.3% of H. diversicolor veliger larvae. Tolerance of C. angulata to ocean acidification was greater than the 2 abalone species; 50.5% of its D-larvae developed normally at 3000 ppm CO2. This apparent resistance of C. angulata to ocean acidification may be attributed to their adaptability to estuarine environments. Mechanisms underlying the resistance to ocean acidification of both abalones requires further investigation. Our results suggest that ocean acidification may decrease the yield of these 3 economically important shellfish if increasing CO2 is a future trend.
    Keywords: Abnormality; Abnormality, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Crassostrea angulata; Development; Fertilization success rate; Fertilization success rate, standard deviation; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Haliotis discus hannai; Haliotis diversicolor; Hatching rate; Hatching rate, standard deviation; Laboratory experiment; Metamorphosis rate; Metamorphosis rate, standard deviation; Mollusca; Mortality/Survival; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Percentage; Percentage, standard deviation; pH; pH, standard deviation; Potentiometric; Potentiometric titration; Reproduction; Salinity; Salinity, standard deviation; Shell length; Shell length, standard deviation; Single species; Species; Stage; Survival; Survival rate, standard deviation; Temperate; Temperature, water; Temperature, water, standard deviation; Time in hours; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 3738 data points
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    DATA PANGAEA
  • 4
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    Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification (2017)
    PANGAEA
    In:  Supplement to: Yang, Bingye; Pu, F; Li, lingling; You, W; Ke, C; Feng, Danqing (2017): Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification. Comparative Biochemistry and Physiology Part B: Biochemistry & Molecular Biology, 206, 8-15, https://doi.org/10.1016/j.cbpb.2017.01.006
    Details
    Publication Date: 2024-03-15
    Description: The formation of the primary shell is a vital process in marine bivalves. Ocean acidification largely influences shell formation. It has been reported that enzymes involved in phenol oxidation, such as tyrosinase and phenoloxidases, participate in the formation of the periostracum. In the present study, we cloned a tyrosinase gene from Crassostrea angulata named Ca-tyrA1, and its potential function in early larval shell biogenesis was investigated. The Ca-tyrA1 gene has a full-length cDNA of 2430 bp in size, with an open reading frame of 1896 bp in size, which encodes a 631-amino acid protein that includes a 24-amino acid putative signal peptide. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that Ca-tyrA1 transcription mainly occurs at the trochophore stage, and the Ca-tyrA1 mRNA levels in the 3000 ppm treatment group were significantly upregulated in the early D-veliger larvae. WMISH and electron scanning microscopy analyses showed that the expression of Ca-tyrA1 occurs at the gastrula stage, thereby sustaining the early D-veliger larvae, and the shape of its signal is saddle-like, similar to that observed under an electron scanning microscope. Furthermore, the RNA interference has shown that the treatment group has a higher deformity rate than that of the control, thereby indicating that Ca-tyrA1 participates in the biogenesis of the primary shell. In conclusion, and our results indicate that Ca-tyrA1 plays a vital role in the formation of the larval shell and participates in the response to larval shell damages in Crassostrea angulata that were induced by ocean acidification.
    Keywords: Alkalinity, total; Animalia; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); Crassostrea angulata; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression; Gene expression, standard deviation; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Registration number of species; Salinity; Single species; Species; Stage; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference; Zooplankton
    Type: Dataset
    Format: text/tab-separated-values, 252 data points
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    DATA PANGAEA
  • 5
    Electronic Resource
    Electronic Resource
    Fluorescent probes for imaging and flow cytometry in living cells
    Amsterdam : Elsevier
    Biology of the Cell 73 (1991), S. 46 
    Details
    ISSN: 0248-4900
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0248-4900(91)90246-J
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    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    2-(2'-Phosphoryloxyphenyl)-4(3H)-quinazolinone derivatives as fluorogenic precipitating substrates of phosphatases
    Amsterdam : Elsevier
    Analytical Biochemistry 207 (1992), S. 32-39 
    Details
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-2697(92)90495-S
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Phallotoxin and actin binding assay by fluorescence enhancement
    Amsterdam : Elsevier
    Analytical Biochemistry 200 (1992), S. 199-204 
    Details
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-2697(92)90299-M
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  • 8
    Electronic Resource
    Electronic Resource
    Theoretical approach to EPR spectrum of C^-"6"0 in molecular sieve 13X
    Amsterdam : Elsevier
    Chemical Physics Letters 213 (1993), S. 373-376 
    Details
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0009-2614(93)85148-H
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Protein bodies in corn endosperm are enclosed by and enmeshed in F-actin (1991)
    Springer
    Protoplasma 165 (1991), S. 139-149 
    Details
    ISSN: 1615-6102
    Keywords: Actin circles ; Corn endosperm ; Fluorescein-S1-myosin ; Protein bodies ; Rhodamine-phalloidin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When corn endosperm is gently homogenized in a microfilament-stabilizing buffer and filtrates centrifuged at very low g forces, abundant protein bodies are present in the pellet. When stained with both rhodamine-phalloidin and fluorescein-S1-myosin and viewed under epifluorescence, individual protein bodies appear to be enclosed in F-actin and groups of protein bodies appear to be enmeshed within a network of fine actin filaments. The yield of actinenclosed protein bodies is not affected by the addition of non-ionic detergents to the grinding medium, but it is severely decreased with the addition of cytoskeleton-disrupting agents including Tris-HCl, KCl, KI, and ammonium sulphate, and the interconnecting fine filaments are totally abolished. Heparin, which interacts with membrane-cytoskeleton complexes, causes apparent coagulation of the protein bodies. The evidence from fluorescent microscopy suggesting that F-actin surrounds and enmeshes the protein bodies is supported by electron microscopy, gel electrophoresis, and Western blot analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322284
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  • 10
    Electronic Resource
    Electronic Resource
    The wavelets and hierarchies of the climate system (1996)
    Springer
    Meteorology and atmospheric physics 61 (1996), S. 19-26 
    Details
    ISSN: 1436-5065
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Summary Wavelet analysis of global mean temperature data, the drought-flood data of the past 500 years in China, and the temperature time series of Shanghai, highlights the following: (1) the climatic wavelets depend on the hierarchies of a climate system; (2) for different time scales or hierarchies, a climate system may have different catastrophic points and periods; (3) for different time scales or hierarchies, variation of the Southern Hemisphere temperature has an obvious phase-lag compared with that of the Northern Hemisphere and the catastrophe points also lag behind; (4) in a cold-warm period, the cold semi-period of the Northern Hemisphere is obviously longer than the warm one; (5) for different hierarchies or time scales, there exists a phase-lag for the droughtflood variation in China moving gradually with latitude from north to south. Finally, a new technique of climate diagnosis, the phase curve of wavelets, is put forward in this paper.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01029708
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