ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Porous hydroxyapatite (HA) scaffolds were processed in hyaluronic acid solution. Bonemarrow cells obtained from the bone shaft of femurs of Fischer 344 rats at 1×106/ml concentrationwere seeded in pores of the scaffolds. The scaffolds were implanted in the dorsal subcutaneoustissue of rats for 2, 4, 6 or 8 weeks. Removed HA scaffolds at 2 and 4 week after dorsalsubcutaneous implantation were histologically examined. At all experimental periods, osteocalcin inthe scaffold was immunochemically measured for the quantitative analysis of osteogenesis by bonemarrow cells in the porous HA scaffolds. Moreover, value of alkaline phosphatase (ALP) activity inthe scaffolds was measured. Osteocalcin measured in scaffolds without bone marrow cells was 1.3ng in an average and the ALP activity was 62.2 μmol at 4 week. In hyaluronic acid processedscaffold with bone marrow cells, quantity of osteocalcin increased from 1.6 ng at 2 week to 2.2 ng at4 week after implantation of the scaffold. Histologically, many pores containing bone in thescaffolds immersed in hyaluronic acid solution were detected. Significant difference of the quantityof osteocalcin was recognized between 2 and 4 week implantation. There was no significantdifference in the quantity of osteocalcin between the scaffolds implanted for 4 and 8 weeks. Valueof ALP activity of the scaffold implanted for 4 weeks showed significant difference comparing withthat implanted for 6 and 8 weeks. From the results of this study, quantitative increase of the boneformation in the pores of HA scaffolds would be able to observe from 6 to 8 weeks afterimplantation on the scaffolds by immersion in hyaluronic acid solution
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1185.pdf
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