Publication Date:
2013-01-01
Description:
Purpose. Truncated tissue factor (tTF) fusion protein targeting tumor vasculature can induce tumor vascular thrombosis and necrosis. Here, we generated (RGD)3-tTF in which three arginine-glycine-aspartic (RGD) targeting integrinαvβ3and tTF induce blood coagulation in tumor vessels.Methods. The bioactivities of (RGD)3-tTF including coagulation activity,FXactivation, and binding with integrinαvβ3were performed. The fluorescent labeled (RGD)3-tTF was intravenously injected into tumor-bearing mice and traced in vivo. The tumor growth, volume, blood vessel thrombosis, tumor necrosis, and survival time of mice treated with (RGD)3-tTF were evaluated.Results. The clotting time andFXactivation of (RGD)3-tTF were similar to that of TF (P〉0.05) but different with that of RGD (P
Print ISSN:
2356-6140
Electronic ISSN:
1537-744X
Topics:
Natural Sciences in General
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