Publikationsdatum:
2005-11-08
Beschreibung:
Because the mucosal epithelia are in constant contact with large numbers of microorganisms, these surfaces must be armed with efficient microbial control systems. Here, we show that the Drosophila nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme, dual oxidase (dDuox), is indispensable for gut antimicrobial activities. Adult flies in which dDuox expression is silenced showed a marked increase in mortality rate even after a minor infection through ingestion of microbe-contaminated food. This could be restored by the specific reintroduction of dDuox, demonstrating that this oxidase generates a unique epithelial oxidative burst that limits microbial proliferation in the gut. Thus, oxidant-mediated antimicrobial responses are not restricted to the phagocytes, but rather are used more broadly, including in mucosal barrier epithelia.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ha, Eun-Mi -- Oh, Chun-Taek -- Bae, Yun Soo -- Lee, Won-Jae -- New York, N.Y. -- Science. 2005 Nov 4;310(5749):847-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Molecular Life Science and Center for Cell Signaling Research, Ewha Woman's University, Seoul 120-750, South Korea.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16272120" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
Animals, Genetically Modified
;
Bacteria/*growth & development/immunology
;
Catalase/genetics/metabolism
;
Chlorides/metabolism
;
Colony Count, Microbial
;
Digestive System/enzymology/immunology/microbiology
;
Drosophila/*enzymology/*immunology/microbiology
;
*Immunity, Innate
;
In Vitro Techniques
;
Intestinal Mucosa/enzymology/immunology/microbiology
;
NADPH Oxidase/genetics/*metabolism
;
RNA Interference
;
Reactive Oxygen Species/metabolism
;
Respiratory Burst
;
Saccharomyces cerevisiae/*growth & development/immunology
;
Superoxides/metabolism
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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