ISSN:
1399-3054
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
Notizen:
The plastid genes psaA, psaB and rps14, encoding the photosystem I reaction center chlorophyll proteins and ribosomal protein CS14, are organized into an operon on the circular plastid genome. To elucidate the regulatory mechanism of platid transcription during plant development, a DNA-binding protein has been characterized and partially purified from spinach (Spinacia oleracea L. var. spinosa Ashers et Graeden) chloroplasts. The protein interacts specifically with promoter sequences from −51 to −17 (named region U) of the operon. The purification procedures include high-salt extraction of chloroplasts, ammonium sulfate fractionation (25–65% saturation), heparin agarose chromatography, heat treatment at 65°C and sequence-specific DNA-affinity chromatography. Gel filtration in combination with Southwestern blot analysis conclude that this DNA-binding protein is dimeric and composed of two 34-kDa subunits. A 28-kDa protein without region U-binding activity was observed to copurify with the DNA-binding protein using DNA-affinity chromatography.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1034/j.1399-3054.1999.106114.x
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