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  • 1
    Monograph available for loan
    Monograph available for loan
    Springende DNA-Elemente in Eukaryoten : [Vortrag, gehalten am 21. März 1985 vor der Klasse Biowissenschaften] (1986)
    Berlin : Akademie-Verl.
    Details Availability
    Call number: MOP Per 672/A
    Type of Medium: Monograph available for loan
    Pages: 21 S. : Ill., graph. Darst.
    ISBN: 3055000072
    Series Statement: Sitzungsberichte der Akademie der Wissenschaften der DDR : N, Mathematik, Naturwissenschaften, Technik 1985, 14
    Location: MOP - must be ordered
    Branch Library: GFZ Library
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  • 2
    Electronic Resource
    Electronic Resource
    People must be judged in the context of their time (2000)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 404 (2000), S. 330-330 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Sir Alison Abbott reports on the Max Planck Society's recent efforts to shed light on its history, as the Kaiser Wilhelm Society, during the Third Reich (Nature 403, 474– 475; 2000). This attempt is all the more ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35006259
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  • 3
    Electronic Resource
    Electronic Resource
    Opportunism knocks? (2000)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 404 (2000), S. 120-120 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Sir Bernd Legler and Guy Moore comment in Correspondence on the evaluation of East German institutes by the Wissenschaftsrat and the intellectual and moral qualities of the scientists examined. First, we are not aware of interviews held in English during evaluation of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35004754
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  • 4
    Electronic Resource
    Electronic Resource
    Normale Zweitbruten beim Rothalstaucher(Podiceps griseigena) (1961)
    Springer
    Journal of ornithology 102 (1961), S. 484-485 
    Details
    ISSN: 1439-0361
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01671018
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  • 5
    Electronic Resource
    Electronic Resource
    Balbiani ring DNA: Sequence comparisons and evolutionary history of a family of hierarchically repetitive protein-coding genes (1984)
    Springer
    Journal of molecular evolution 20 (1984), S. 281-295 
    Details
    ISSN: 1432-1432
    Keywords: Balbiani ring ; Repeat ; Evolution ; Repetitive DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary All known types of Balbiani ring (BR) gene consist of multiple, tandemly arranged, ca. 180 to 300-bp repeat units that can be divided into a constant region and a subrepeat region. The latter region includes short tandem subrepeats (SRs). Comparison of all available BR sequences using computer methods has enabled us (a) to define more precisely the constant and subrepeat regions, (b) to infer the evolutionary relationships among the various types of BR repeats, (c) to derive a consensus approximation of an ancestral sequence from a small segment of which the highly diverse present-day SRs may have originated, and (d) to detect an underlying substructure in the constant region, evident in the consensus but not in the present-day sequences and possibly corresponding to an original 39-bp DNA segment from which the extant, giant BR sequences may have evolved. We discuss the processes of reduplication, diversification, and homogenization within the hierarchically repetitive BR sequences as examples of how a simple DNA element may evolve into a diverse family of large, protein-coding genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02104734
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  • 6
    Electronic Resource
    Electronic Resource
    The 3′ ends of two genes in the Balbiani ring c locus ofChironomus thummi (1986)
    Springer
    Journal of molecular evolution 24 (1986), S. 72-82 
    Details
    ISSN: 1432-1432
    Keywords: Balbiani ring ; Chironomus ; DNA sequence comparison ; Gene conversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 3′-end sequences of two nonallelic genes derived from the Balbiani ring c (BRc) locus ofChironomus thummi are described. Only one of the genes appears to be transcribed abundantly in normal late larval salivary glands. The two sequences are highly similar, even in the 3′ untranslated regions, but sharply diverge beyond the polyadenylation site. Together with evidence from the 3′ ends of BR1 and BR2 genes ofC. pallidivittatus andC. tentans, independently characterized by others, this result suggests the existence of a sequence-homogenization mechanism that operates across the 3′ ends of all BR genes characterized to date. The 3′-terminal coding region of each BRc gene is divided into two portions by a short intron. The upstream portion is homologous to and continuous with the tandem repeats that make up the internal core of each BR gene; however, that portion is variant in sequence relative to the core, and apparently is not subject to the homogenization process that operates on the core repeats. The portion downstream of the intron encodes a unique, 111-residue polypeptide highly different from the rest of the BRc product. The evolution of the various segments of the BRc genes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02099953
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  • 7
    Electronic Resource
    Electronic Resource
    Chromosomal localization of ribosomal 5 S RNA genes in Chironomus thummi by in situ hybridization of iodinated 5S RNA (1976)
    Springer
    Chromosoma 57 (1976), S. 199-204 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract 5 S RNA of Chironomus thummi larvae was purified from total phenol extracted RNA by gel filtration and labelled to about 107 dpm/μg with carrier-free iodine-125. After hybridization in situ of 125I-5S RNA and autoradiography only region B3c-e (containing two “normal” and two very faint bands) of chromosome II of salivary gland cells was highly labelled. In chromosomes of an animal showing pairing discontinuities a clearly “heterozygous” labelling of the 5 S RNA region was found. Region B3c-e shows no clearcut morphological signs of puffing or autoradiographically detectable 3H-uridine incorporation in spite of a continuous synthesis of 5 S RNA in salivary gland cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292918
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  • 8
    Electronic Resource
    Electronic Resource
    Phosphoenolpyruvate carboxylase in developing seeds of Vicia faba L.: gene expression and metabolic regulation (1999)
    Springer
    Planta 208 (1999), S. 66-72 
    Details
    ISSN: 1432-2048
    Keywords: Key words: C/N interaction ; Phosphoenolpyruvate carboxylase (gene expression ; metabolic regulation) ; Vicia seed development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. To analyze the role of phosphoenolpyruvate carboxylase (PEPCase, EC 4.1.1.31) during seed development, two cDNA clones encoding two isoforms of PEPCase were isolated from a seed-specific library of Vicia faba. The two sequences (VfPEPCase1 and VfPEPCase2) have a sequence identity of 82 and 89% on the nucleotide and amino acid levels. The VfPEPCase1 mRNA was found to be predominantly expressed in roots and developing cotyledons whereas the VfPEPCase2 mRNA was more abundant in green and maternal tissues. In the cotyledons, PEPCase mRNAs accumulated from early to mid cotyledon stage and decreased thereafter. The PEPCase activity increased continuously during cotyledon development. The enzyme was strongly activated by glucose-6-phosphate, but not by glucose, fructose or sucrose. Asparagine was weakly activating whereas malate, aspartate and glutamate were inhibitory. The inhibitors became less effective with increasing pH. Aspartate was a much stronger inhibitor of cotyledonary PEPCase than glutamate at both pH 7.0 and 7.5. The sensitivity of PEPCase to malate inhibition decreased from early to mid cotyledon stage at a time when storage proteins are synthesized. This indicates activation on the protein level, possibly by protein phosphorylation. Nitrogen starvation in the presence of hexoses but not sucrose decreased mRNA levels of VfPEPCase1 and enzyme activity, indicating control on the mRNA level by both carbon and nitrogen. It is concluded that in developing cotyledons PEPCase is probably important for the synthesis of organic acids to provide carbon skeletons for amino acid synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050535
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  • 9
    Electronic Resource
    Electronic Resource
    Sugar levels altered by ectopic expression of a yeast-derived invertase affect cellular differentiation of developing cotyledons of Vicia narbonensis L. (2000)
    Springer
    Planta 211 (2000), S. 325-334 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Carbohydrate state ; Cellular differentiation ; Invertase ; Storage protein ; Vicia (seed development)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract.  In order to change the sugar status during seed development a yeast-derived invertase gene was expressed in cotyledons of Vicia narbonensis. As a result, sucrose decreased whereas hexoses accumulated. We analysed cell structure and cellular differentiation in cotyledons expressing the yeast-invertase. Transgenic cells contained large and long-persisting vacuoles apparently serving as storage compartments for hexoses and clusters of storage-protein aggregates. In the wild-type, large vacuoles did not persist but were replaced by smaller protein bodies. During maturation and desiccation, the transgenic cells showed plasmolysis and vesiculation of the endo-membrane system. Immunogold-labelling revealed that the storage proteins vicilin and legumin were present within the cytoplasm and the extraprotoplasmic space and were attached to membranes of the endoplasmic reticulum and the nuclei. Protein storage vacuoles in mature seeds appeared heterogeneous and only partially filled. The data suggest that sugars control the subcellular organisation of the vacuolar system. Transcript levels encoding a tonoplast intrinsic protein, a marker for membranes of protein storage vacuoles, remained unchanged whereas mRNA levels of a hexose and a sucrose transporter increased. Generally, transgenic seeds appeared to be physiologically younger than wild-type seeds of the same age. The data underline the important role of sugars in legume seed development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250000305
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  • 10
    Electronic Resource
    Electronic Resource
    A sucrose-synthase gene of Vicia faba L.: Expression pattern in developing seeds in relation to starch synthesis and metabolic regulation (1993)
    Springer
    Planta 191 (1993), S. 394-401 
    Details
    ISSN: 1432-2048
    Keywords: cDNA clone (sucrose synthase) ; Gene expression (sucrose synthase, seed development) ; Nucleotide sequence (sucrose synthase) ; Seed development ; Sucrose synthase (cloning, expression, regulation) ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Copy-DNA clones encoding a single class of sucrose-synthase (SUCS; EC 2.4.1.13) subunit have been isolated and sequenced from a Vicia faba L. seed cotyledonary library. Southern analyses indicated the existence of only one gene. Transcript levels determined by Northern blot hybridisation steadily increased until the middle of development [25–35 days after flowering (DAF)] and declined thereafter. Sucrose levels approximately paralleled levels of SUCS mRNA. The activity of SUCS increased with decreasing fructose and glucose concentrations and peaked about 10 d later than mRNA levels. In-vitro culture experiments demonstrated that increasing the sucrose concentration leads to increased levels of SUCS mRNA. The SUCS mRNA was also synthesised in seed-coat tissue, but in lower amounts than in cotyledons and with a different developmental profile. The early peak level of SUCS mRNA (20 DAF) in seed coats coincided with the peak in the amount of sucrose and with a peak of transiently synthesised starch.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195698
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