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  • 1
    Electronic Resource
    Electronic Resource
    Seed protein and isozyme variations in Triticum tauschii (Aegilops squarrosa) (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 257-263 
    Details
    ISSN: 1432-2242
    Keywords: Triticum tauschii ; Genetic variability ; HMW-glutenins ; Gliadins ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sixty Triticum tauschii (Aegilops squarrosa, 2n=2x=14, DD) accessions were evaluated for the variability of high-molecular-weight (HMW) glutenins, gliadins and isozymes of seed esterase, β-amylase and glucose-phosphate isomerase. Wide variability was observed for HMW-glutenins and gliadins. The implications of unique HMW-glutenin alleles for quality parameters are discussed. Isozyme evaluations indicated more variability for the Est-D t 5 locus as compared to the Est-D5 of bread-wheat. The polymorphism for β-Amy-D t 1 was less than that of β-Amy-D1. Similar to the bread-wheat situation, Gpi-D t 1 showed no polymorphism. The variability observed with the traits evaluated can be readily observed in T. turgidum x T. tauschii synthetic hexaploids (2n=6x=42, AABBDD) suggesting that T. tauschii accessions may be a rich source for enhancing the genetic variability of T. aestivum cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223774
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  • 2
    Electronic Resource
    Electronic Resource
    Isozyme and cytological markers of some Psathyrostachys juncea accessions (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 528-534 
    Details
    ISSN: 1432-2242
    Keywords: Psathyrostachys juncea ; Triticum aestivum ; Isozyme markers ; Chromosome banding ; Intergeneric hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Psathyrostachys juncea (synonymous to Elymus junceus; 2n=2x=14, NN) has unique biotic and abiotic attributes that could contribute towards wheat improvement. The effectiveness of such an intergeneric hybridization program depends greatly on being able to establish diagnostic markers of the alien chromosomes. Isoelectric focusing (IEF) analyses of six enzyme systems have identified five biochemical markers — malate dehydrogenase (MDH), esterase (EST), shikimate dehydrogenase (SKDH), phosphoglucomutase (PGM), and β-amylase (β-AMY) — to be of positive diagnostic value; glucosephosphate isomerase (GPI) banding profiles were of no definite value in the background of Triticum aestivum cvs ‘Chinese Spring’ and ‘Seri-82’, the potential recipients of Ps. juncea chromosomes. The Giemsa C-banding karyotype distinctively separates the Ps. Juncea chromosomes from each other and from those of T. aestivum with little banding site polymorphisms prevalent among its accessions analyzed, indicating the usefulness of C-bands as cytological markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224148
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  • 3
    Electronic Resource
    Electronic Resource
    Thinopyrum bessarabicum: biochemical and cytological markers for the detection of genetic introgression in its hybrid derivatives with Triticum aestivum L. (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 365-370 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Thinopyrum bessarabicum ; Protein-isozyme markers ; Chromosome banding ; Intergeneric hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thinopyrum bessarabicum (2n = 2x = 14, JJ) with its unique property of salt tolerance provides a potential means for the transfer of this important and complex trait into cultivated wheat through intergeneric hybridization. To accomplish this, diagnostic markers for detecting the presence of Th. bessarabicum chromosomes in a wheat background have to be established. The C-banded karyotype of Th. bessarabicum distinctly identifies individual Th. bessarabicum chromosomes and separates them from those of Triticum aestivum. Also, seven protein/isozymes, i.e., malate dehydrogenase, high-molecular-weight glutenin, Superoxide dismutase, grain esterase, glutamate oxaloacetate transaminase, β-amylase and α-amylase, were identified as being positive markers specific to Th. bessarabicum; these were also expressed in the T. aestivum/Th. bessarabicum amphiploid. These diagnostic biochemical markers could be useful in detecting and establishing homoeology of Th. bessarabicum chromosomes in T. aestivum/Th. bessarabicum intergeneric hybrid derivatives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222103
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  • 4
    Electronic Resource
    Electronic Resource
    Biochemical and molecular diagnostics of Thinopyrum bessarabicum chromosomes in Triticum aestivum germ plasm (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 952-956 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Thinopyrum bessarabicum ; Protein/isozyme markers ; In situ hybridization ; Alien disomic additions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thinopyrum bessarabicum (2n=2x=14, JJ) is a self-fertile salt-tolerant grass species, and its hybridization with Triticum aestivum to achieve the transfer of this attributes has been promoted. For the detection of alien introgression, development of diagnostic markers of Th. bessarabicum chromosomes in the wheat background has emerged as an important aspect in our intergeneric hybridization program. Six proteins/isozymes-high-molecular-weight glutenins, superoxide dismutase, grain esterase, β-amylase, glutamate oxaloacetate transaminase and α-amylase —were identified as positive markers for detecting the presence of Th. bessarabicum chromosomes in the advanced backcross derivatives of T. aestivum/Th. bessarabicum//n* T. aestivum. Fluorescent in situ hybridization further enabled the detection of complete and translocated arms of Th. bessarabicum chromosomes in the T. aestivum background. These diagnostic markers served for tentatively characterizing a distinct set of Th. bessarabicum disomic additions to wheat (2n=44) and have facilitated establishing the homoeology of these added chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222908
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