ISSN:
1617-4623
Keywords:
CryIIB
;
δ-Endotoxin
;
Cryptic gene
;
Operon
;
Chaperone
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A δ-endotoxin gene previously cloned from Bacillus thuringiensis subsp. galleriae has been shown by a combination of restriction mapping and DNA sequence analysis to be a cryIIB clone; in common with other cryIIB genes it was found to lack a functional promoter. Addition of a promoter resulted in expression of the gene in Bacillus thuringiensis but did not result in the formation of the crystalline inclusions normally associated with such toxins. Inclusion formation was only observed when the gene was incorporated into an operon containing a gene known to be involved in the crystallisation of another δ-endotoxin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00280428
Permalink