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  • 1
    Electronic Resource
    Electronic Resource
    Genetic and cytological characterisation of fusion chromosomes of Dictyostelium discoideum (1981)
    Springer
    Chromosoma 82 (1981), S. 321-332 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Abnormally large chromosomes which appear to result from the fusion of 2 chromosomes of the normal karyotype have been found in diploids of Dictyostelium discoideum formed by parasexual fusion of haploid strains HU483 (n=7) and HU245 (n=7). These fusion chromosomes appear to be the products of the tandem translocation of most, if not all, of one acrocentric chromosome to the telomere of a second acrocentric. Thus the chromosome number of the diploids is reduced from the normal 2n=14 to 2n=13 with the formation of an abnormally large acrocentric fusion chromosome. Experimental haploidisation of such diploids results in two types of products, those with a normal 7 chromosome karyotype and those with an abnormal 6 chromosome karyotype which contains the fusion chromosome. Genetic analysis of haploid segregants indicates that linkage groups II and VII are involved in this fusion. Phenotypes of recombinant diploids obtained following mitotic crossing-over establishes that linkage group II is proximal to linkage group VII. Cytological examination of the karyotypes of haploid strains bearing the fusion chromosome suggest that chromosome 2 may correspond to linkage group II and chromosome 3 to linkage group VII. Haploid strains bearing the fusion chromosome grow and develop normally so little or no genetic information can have been lost in the fusion event. While the nature of this event is unknown it may have involved aberrant recombinational DNA repair since the parental haploid strain HU483 bears the radB13 DNA repair mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285758
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  • 2
    Electronic Resource
    Electronic Resource
    Dictyostelium discoideum nuclear plasmid Ddp6 is a new member of the Ddp2 plasmid family (1998)
    Springer
    Current genetics 33 (1998), S. 77-82 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsDictyostelium discoideum ; Cellular slime mold ; Nuclear plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ddp6 is a high-copy number, circular plasmid found in the nucleus of the simple eukaryote Dictyostelium discoideum wild isolate NC47.2. The complete nucleotide sequence, 5257 bp, shows that Ddp6 has a structure similar to that of other members of the Ddp2 plasmid family: a single long 2.8-kb open reading frame (rep gene) and an inverted repeat containing a pair of 654-bp elements. A single constitutively expressed 3.3-kb transcript of the rep gene was detected in RNA prepared from vegetative and developmental cells. Maintenance assays revealed that sequences within the inverted repeat and the intact Ddp6 ORF are essential for maintenance of the plasmid. Mutation of the inverted repeat, or of the rep gene, lowered the plasmid copy number but did not affect autonomous replication of shuttle-vector constructs. Comparisons of the predicted protein products of the rep genes of five members of the Ddp2 plasmid family identified a set of ten conserved features distributed throughout the peptides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050311
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  • 3
    Electronic Resource
    Electronic Resource
    In vivo nicking and rejoining of nuclear DNA in ultraviolet-irradiated radiation-resistant and sensitive strains of Dictyostelium discoideum (1979)
    Springer
    Molecular genetics and genomics 167 (1979), S. 259-263 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Some aspects of DNA repair in several radiation-resistant and radiation-sensitive strains of Dictyostelium discoideum were investigated by using alkaline sucrose gradients to analyze for the production and resealing of single-strand breaks following irradiation with 254 nm UV. All radiation-resistant strains and all mutants assayed that are sensitive to both UV and 60Co gamma rays produced singlestrand breaks in their nuclear DNA after a UV fluence of 15 J/m2. Mutants at the radC locus which are sensitive to UV but as resistant as their parental strains to 60Co gamma rays produced many fewer single-strand breaks in their DNA after irradiation with UV. Thus, the radC mutations alter a repair pathway specific for UV-induced DNA damage and presumably affect the activity of a UV-damage-specific endonuclease involved in excision repair. All radiation-resistant strains and all of our mutants sensitive to gamma rays rejoined much of their DNA during a three-hour post-UV-irradiation incubation, suggesting that these strains have at least a partially intact excision repair system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267417
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  • 4
    Electronic Resource
    Electronic Resource
    Dictyostelium discoideum cobB mutants show reduced heavy metal accumulation associated with gene amplification (1996)
    Springer
    Molecular genetics and genomics 253 (1996), S. 65-73 
    Details
    ISSN: 1617-4623
    Keywords: Keywords Dictyostelium discoideum ; Heavy metal resistance ; Extrachromosomal DNA ; Gene amplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Wild-type Dictyostelium discoideum cells grow- ing on non-toxic levels of nickel chloride or cobaltous chloride accumulate 2–3.5 times as much nickel and at least 1.5 times as much cobalt as cobB mutants. The cobB trait is dominant, confers unstable cobalt and nickel resistance and is correlated with the presence of up to 50 copies of a linear extrachromosomal DNA, approximately 100 kb in length, derived from linkage group III. Independent cobB mutants can be obtained by selection on medium containing either cobalt or nickel. The amplified DNA can be transferred to wild-type strains by electroporation. Strains with mutations at a second cobalt resistance locus, cobA, accumulate the same amount of cobalt, but more nickel than wild-type strains. Our results are consistent with the cobA mutant phenotype being due to internal sequestration of cobalt, and the cobB mutant phenotype being due to reduced net uptake of cobalt and nickel. Energy-dependent nickel export was detectable in wild-type and cobB mutant strains but its role in heavy metal resistance has not yet been proved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050297
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  • 5
    Electronic Resource
    Electronic Resource
    Interactions between radiation-sensitive mutations in double-mutant haploids of Dictyostelium discoideum (1979)
    Springer
    Molecular genetics and genomics 167 (1979), S. 265-270 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Haploid strains of Dictyostelium discoideum carrying radiation-sensitive mutations in both the radA and radC genes are more sensitive to UV light irradiation than the “additive” sensitivity of the single-mutant haploids. This synergistic interaction indicates that the radA and radC gene products are involved in two different pathways of repair following UV-induced DNA damage. Double-mutant haploids bearing mutations in both the radA and radB genes have the same sensitivity as the radB single mutant indicating that the radA and radB gene products are involved in the same repair pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267418
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  • 6
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    Genetic and cytological characterisation of fusion chromosomes of Dictyostelium discoideum (1981)
    Springer
    Details
    Publication Date: 1981-04-01
    Print ISSN: 0009-5915
    Electronic ISSN: 1432-0886
    Topics: Biology , Medicine
    Published by Springer
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  • 7
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    Dictyostelium discoideum nuclear plasmid Ddp6 is a new member of the Ddp2 plasmid family (1998)
    Springer
    Details
    Publication Date: 1998-02-11
    Print ISSN: 0172-8083
    Electronic ISSN: 1432-0983
    Topics: Biology
    Published by Springer
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