ISSN:
1612-1112
Keywords:
Sialyl compounds
;
Uremic toxins
;
Hemofiltration
;
Renal failure
;
NMR-spectroscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Hemofiltrate of patients with end stage renal disease is a source of sialyl compounds of unusual composition which accumulate in uremia. The large volumes involved (20–30l), are concentrated and desalted by modified reverse osmosis using membranes with a nominal cut-off of 500 D. The transport of the individual solutes during this process is monitored by reversed-phase HPLC of the permeates and retentates, respectively. By a combination of size-exclusion and ion-exchange chromatography 11 sialyl compounds are isolated preparatively. All structures have been determined using one and two dimensional NMR spectroscopy, enzymatic degradation and FAB mass spectrometry.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02290384
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