ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The purpose of this study is to study the proliferous effect of mandibular condylarchondrocytes given static tension-stress and/or transforming growth factor-β1 (TGF-β1) in vitro. Thefourth-passage condylar chondrocytes were harvested for this study, and a pulsatile cellularmechanical system was used to apply stress on cells. The proliferous effect of condylarchondrocytes given continuous static tension-stress and/or TGF-β1 were examined by using flowcytometry. The experiment was divided into two parts. The first part was divided into 20 groupsaccording to different TGF-β1 dosage (0ng/ml, 0.1ng/ml, 1ng/ml and 10ng/ml) for 0, 6, 12, 18 and24 hours respectively. The second part was divided into eight groups under continuous statictension-stress (0 or 5kPa) and different TGF-β1 dosage (0ng/ml, 0.1ng/ml, 1ng/ml, 10ng/ml) for 12hours. Experimental data was analyzed with repeated interclass analysis of variance The resultsshowed that chondrocytes which were cultured under different TGF-β1dose combined with 5kPastatic tension-stress had multi-horn morphological characters, including a great quantity ofchondrocytes with division growth.TGF-β1 had a mitogenic effect on rat mandibular condylechondrocytes at the concentrations of 0.1 , 1 and 10ng/ml , and the mitogenic effect of TGF-β1 tocondylar chondrocytes were demonstrated after 12 to 18 hours, and the peak of mitogenic effectsappeared at the 18th hour (P 〈0.05) . The most active mitogenesis happened in the group whosechondrocytes was under continuous static tension-stress (5kPa) combined with TGF-β1. Theseresults proved that mechanical stimulus and TGF-β1 in vitro could influence and regulate thegrowth of condylar chondrocytes
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1125.pdf
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