ISSN:
0741-0581
Keywords:
Virus
;
Electron microscopic autoradiography
;
Pathology
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
To determine the pathogenesis of herpes cervicitis and the relationship between herpes simplex virus type 2 (HSV-2) and the carcinogenesis of human uterine cervix, the ultrastructural changes of 15 cases of long-term organ cultures of human uterine cervix infected with HSV-2 were studied. In order to demonstrate the morphogenesis of the virus and the interactions between virus and host cells, EM autoradiography was performed. After viral inoculation, the course of viral DNA replication, and the processes of maturation of virus were examined. Pathological alterations of infected nuclei-including two kinds of granular matrix aggregations, proliferative changes in nuclei and nuclear envelope, multi-lobular nucleated giant cell formation, intranuclear microtubules, and some evidence of replication of HSV-2 in cytoplasm-were observed. Because of the similarity of the pathologic alterations and the course and duration of infection in the clinic cases and our experimental subjects, this system appears to be a good model to investigate the pathogenesis of herpes cervicitis and the relationship between human cervical cancer and HSV-2, other viruses, or other co-carcinogens.
Additional Material:
16 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060070205
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