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  • 1
    Electronic Resource
    Electronic Resource
    High-resolution mass spectrometric determination of polychlorinated dibenzo-P-dioxins and dibenzofurans using an alternative lockmass system (1988)
    s.l. : American Chemical Society
    Analytical chemistry 60 (1988), S. 1429-1433 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00165a016
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  • 2
    Electronic Resource
    Electronic Resource
    XPN-FMS: A CAD tool for FMS modeling, analysis, animation, and simulation using Petri nets and X window (1995)
    Springer
    International journal of flexible manufacturing systems 7 (1995), S. 339-360 
    Details
    ISSN: 1572-9370
    Keywords: Petri net ; FMS ; modeling ; simulation ; tool ; analysis ; animation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract We propose a CAD tool, XPN-FMS, which is primarily based on a unique Petri net (PN) synthesis method, called the knitting technique, developed by the authors. Petri net theory has been applied to specification, validation, performance analysis, control code generation, and simulation for manufacturing systems. The analysis of flexible manufacturing systems (FMSs) based on PNs suffers from the complexity problem of reachability analysis (Peterson, 1981). CAD tools are urgently needed. There is no existing CAD tool for FMSs as comprehensive as XPN-FMS, in the sense that the latter integrates the functions of drawing, analysis, reduction (Chao and Wang, 1992; Murata and Koh, 1980), synthesis, property queries, and animation of FMS operations in one software package. Using the X window graphical interface and animation, XPN-FMS makes the modeling and analysis of an FMS visualizable and easy to understand and manipulate. It lets a user draw the factory layout of an FMS on the screen of a monitor using the supplied tools. A corresponding PN model can also be drawn on the monitor screen. XPN-FMS can animate and simulate the overall operating process of the FMS. It is useful for FMS specification, validation, and exploration of different design alternatives, status monitoring, and control. Using XPN-FMS with various inputs and comparing the resulting outputs, the user can determine how to improve efficiency, reduce cost, and pinpoint bottlenecks. For the PN models of FMSs that are decision free, we extend the theory and algorithm of a unique matrix-based method (Chao and Wang, 1993b) to search for subcritical loops (including types A and B) and to support scheduling and dealing with transition periods. XPN-FMS implements this extended method to find the minimum cycle time, critical loop, subcritical loops, next critical loop, and scheduling ranges to avoid the transient period for static scheduling. This is implemented in XPN-FMS for the input sequence control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01325064
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  • 3
    Electronic Resource
    Electronic Resource
    A synthesis technique of general petri nets (1994)
    Springer
    Journal of systems integration 4 (1994), S. 67-102 
    Details
    ISSN: 1573-8787
    Keywords: Concurrent system ; flexible manufacturing system ; ordinary Petri nets ; general Petri nets ; liveness boundedness ; deadlock ; reversible ; synthesis ; structural relationship ; rule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract The general Petri net (GPN) is useful for modeling flexible manufacturing systems with multiple robots and workstations [15] and for parallel programs [8]. A problem of using reachability analysis for analyzing Petri nets (PN) is the large number of states generated. Most of the existing synthesis techniques do not deal with GPN. Koh et al.[15] invented a synthesis technique for GPN. We propose to improve their achievement by adding the simple Arc-ratio rules to Yaw's knitting technique [37, 38, 39] based on the notion of structure relationship together with new path generations, which mark the most distinct feature compared with other approaches. The synthesis rules and procedures of how to update the temporal matrix and structure synchronic distance are presented. The Arc-ratio rules for GPN are also presented. One can successfully synthesize complicated Petri nets using these rules. An example to synthesize a Petri net in [15] is illustrated. The correctness of each synthesis rule with an appropriate Arc-ratio rule for GPN is proved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01975673
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  • 4
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    In-situ mechanical weakness of subducting sediments beneath a plate boundary décollement in the Nankai Trough (2018)
    Springer
    In:  EPIC3Progress in Earth and Planetary Science, Springer
    Details
    Publication Date: 2018-11-12
    Description: The study investigates the in-situ strength of sediments across a plate boundary décollement using drilling parameters recorded when a 1180-m-deep borehole was established during International Ocean Discovery Program (IODP)Expedition 370, Temperature-Limit of the Deep Biosphere off Muroto (T-Limit). Information of the in-situ strength of the shallow portion in/around a plate boundary fault zone is critical for understanding the development of accretionary prisms and of the décollement itself. Studies using seismic reflection surveys and scientific ocean drillings have recently revealed the existence of high pore pressure zones around frontal accretionary prisms, which may reduce the effective strength of the sediments. A direct measurement of in-situ strength by experiments, however, has not been executed due to the difficulty in estimating in-situ stress conditions. In this study, we derived a depth profile for the in-situ strength of a frontal accretionary prism across a décollement from drilling parameters using the recently established equivalent strength (EST) method. At site C0023, the toe of the accretionary prism area off Cape Muroto, Japan, the EST gradually increases with depth but undergoes a sudden change at ~ 800 mbsf, corresponding to the top of the subducting sediment. At this depth, directly below the décollement zone, the EST decreases from ~ 10 to 2 MPa, with a change in the baseline. This mechanically weak zone in the subducting sediments extends over 250 m (~ 800–1050 mbsf), corresponding to the zone where the fluid influx was discovered, and high-fluid pressure was suggested by previous seismic imaging observations. Although the origin of the fluids or absolute values of the strength remain unclear, our investigations support previous studies suggesting that elevated pore pressure beneath the décollement weakens the subducting sediments.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 5
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    Temperature limits to deep seafloor life in the Nankai Trough subduction zone (2020)
    AMER ASSOC ADVANCEMENT SCIENCE
    In:  EPIC3Science, AMER ASSOC ADVANCEMENT SCIENCE, 370(6521), pp. 1230-1234
    Details
    Publication Date: 2020-12-04
    Description: Microorganisms in marine subsurface sediments substantially contribute to global biomass.Sediments warmer than 40°C account for roughly half the marine sediment volume, but theprocesses mediated by microbial populations in these hard-to-access environments are poorlyunderstood. We investigated microbial life in up to 1.2-kilometer-deep and up to 120°C hotsediments in the Nankai Trough subduction zone. Above 45°C, concentrations of vegetativecells drop two orders of magnitude and endospores become more than 6000 times more abundantthan vegetative cells. Methane is biologically produced and oxidized until sediments reach 80°to 85°C. In 100° to 120°C sediments, isotopic evidence and increased cell concentrationsdemonstrate the activity of acetate-degrading hyperthermophiles. Above 45°C, populated zonesalternate with zones up to 192 meters thick where microbes were undetectable
    Repository Name: EPIC Alfred Wegener Institut
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  • 6
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    Expedition 370 Preliminary Report: Temperature Limit of the Deep Biosphere off Muroto. (2017)
    International Ocean Discovery Program
    In:  EPIC3International Ocean Discovery Program, ISSN: 2372-9562
    Details
    Publication Date: 2017-07-31
    Description: International Ocean Discovery Program (IODP) Expedition 370 aimed to explore the limits of life in the deep subseafloor biosphere at a location where temperature increases with depth at an intermediate rate and exceeds the known temperature maximum of microbial life (~120°C) at the sediment/basement interface ~1.2 km below the seafloor. Drilling Site C0023 is located in the vicinity of Ocean Drilling Program (ODP) Sites 808 and 1174 at the protothrust zone in the Nankai Trough off Cape Muroto at a water depth of 4776 m. ODP Leg 190 in 2000, revealed the presence of microbial cells at Site 1174 to a depth of ~600 meters below seafloor (mbsf), which corresponds to an estimated temperature of ~70°C, and reliably identified a single zone of higher cell concentrations just above the décollement at around 800 mbsf, where temperature presumably reached 90°C; no cell count data was reported for other sediment layers in the 70°–120°C range, because the limit of manual cell count for low-biomass samples was not high enough. With the establishment of Site C0023, we aimed to detect and investigate the presence or absence of life and biological processes at the biotic–abiotic transition with unprecedented analytical sensitivity and precision. Expedition 370 was the first expedition dedicated to subseafloor microbiology that achieved time-critical processing and analyses of deep biosphere samples by simultaneous shipboard and shore-based investigations. Our primary objectives during Expedition 370 were to study the relationship between the deep subseafloor biosphere and temperature. We aimed to comprehensively study the factors that control biomass, activity, and diversity of microbial communities in a subseafloor environment where temperatures increase from ~2°C at the seafloor to ~120°C at the sediment/basement interface and thus likely encompasses the biotic–abiotic transition zone. We also aimed to determine geochemical, geophysical, and hydrogeological characteristics in sediment and the underlying basaltic basement and elucidate if the supply of fluids containing thermogenic and/or geogenic nutrient and energy substrates may support subseafloor microbial communities in the Nankai accretionary complex. To address these primary scientific objectives and questions, we penetrated 1180 m and recovered 112 cores across the sediment/basalt interface. More than 13,000 samples were collected, and selected samples were transferred to the Kochi Core Center by helicopter for simultaneous microbiological sampling and analysis in laboratories with a super-clean environment. Following the coring operations, a temperature observatory with 13 thermistor sensors was installed in the borehole to 863 mbsf.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Miscellaneous , notRev
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  • 7
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    The geochemistry of methane isotopologues (2017)
    Massachusetts Institute of Technology and Woods Hole Oceanographic Institution
    Details
    Publication Date: 2022-05-25
    Description: Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution June 2017
    Description: This thesis documents the origin, distribution, and fate of methane and several of its isotopic forms on Earth. Using observational, experimental, and theoretical approaches, I illustrate how the relative abundances of 12CH4, 13CH4, 12CH3D, and 13CH3D record the formation, transport, and breakdown of methane in selected settings. Chapter 2 reports precise determinations of 13CH3D, a “clumped” isotopologue of methane, in samples collected from various settings representing many of the major sources and reservoirs of methane on Earth. The results show that the information encoded by the abundance of 13CH3D enables differentiation of methane generated by microbial, thermogenic, and abiogenic processes. A strong correlation between clumped- and hydrogen-isotope signatures in microbial methane is identified and quantitatively linked to the availability of H2 and the reversibility of microbially-mediated methanogenesis in the environment. Determination of 13CH3D in combination with hydrogen-isotope ratios of methane and water provides a sensitive indicator of the extent of C–H bond equilibration, enables fingerprinting of methane-generating mechanisms, and in some cases, supplies direct constraints for locating the waters from which migrated gases were sourced. Chapter 3 applies this concept to constrain the origin of methane in hydrothermal fluids from sediment-poor vent fields hosted in mafic and ultramafic rocks on slow- and ultraslow-spreading mid-ocean ridges. The data support a hypogene model whereby methane forms abiotically within plutonic rocks of the oceanic crust at temperatures above ca. 300 C during respeciation of magmatic volatiles, and is subsequently extracted during active, convective hydrothermal circulation. Chapter 4 presents the results of culture experiments in which methane is oxidized in the presence of O2 by the bacterium Methylococcus capsulatus strain Bath. The results show that the clumped isotopologue abundances of partially-oxidized methane can be predicted from knowledge of 13C/12C and D/H isotope fractionation factors alone.
    Description: The research activities documented in this thesis were made possible by grants to my advisor from the U.S. National Science Foundation (NSF award EAR-1250394), the National Aeronautics and Space Administration (NASA) Astrobiology Institute (NAI, University of Colorado, Boulder, CAN 7 under Cooperative Agreement NNA15BB02A), the Department of Energy (DOE, Small Business Innovation Research program, contract DE-SC0004575), the Alfred P. Sloan Foundation via the Deep Carbon Observatory, and a Shell Graduate Fellowship through the MIT Energy Initiative. I completed the bulk of the work in this thesis while being supported by a National Defense Science and Engineering Graduate (NDSEG) Fellowship awarded through the Office of Naval Research of the U.S. Department of Defense. The StanleyW.Watson Fellowship Fund provided support during my first summer term at WHOI.The Charles M. Vest Presidential Fellowship at MIT supported me in the first year of my Ph.D. studies. I received additional support that year through NSF award EAR-1159318 (to S. Ono and T. Bosak) and theWalter & Adel Hohenstein Graduate Fellowship of Phi Kappa Phi. The MIT Earth Resources Laboratory and PAOC Houghton Fund funded my attendance at several conferences.
    Keywords: Methane ; Chemistry ; Isotopes ; Oxidation
    Repository Name: Woods Hole Open Access Server
    Type: Thesis
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  • 8
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    Experimental investigation on the controls of clumped isotopologue and hydrogen isotope ratios in microbial methane (2018)
    Details
    Publication Date: 2022-05-25
    Description: Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Geochimica et Cosmochimica Acta 237 (2018): 339-356, doi:10.1016/j.gca.2018.06.029.
    Description: The abundance of methane isotopologues with two rare isotopes (e.g., 13CH3D) has been proposed as a tool to estimate the temperature at which methane is formed or thermally equilibrated. It has been shown, however, that microbial methane from surface environments and from laboratory cultures is characterized by low 13CH3D abundance, corresponding to anomalously high apparent 13CH3D equilibrium temperatures. We carried out a series of batch culture experiments to investigate the origin of the non-equilibrium signals in microbial methane by exploring a range of metabolic pathways, growth temperatures, and hydrogen isotope compositions of the media. We found that thermophilic methanogens (Methanocaldococcus jannaschii, Methanothermococcus thermolithotrophicus, and Methanocaldococcus bathoardescens) grown on H2+CO2 at temperatures between 60 and 80°C produced methane with Δ13CH3D values (defined as the deviation from stochastic abundance) of 0.5 to 2.5‰, corresponding to apparent 13CH3D equilibrium temperatures of 200 to 600°C. Mesophilic methanogens (Methanosarcina barkeri and Methanosarcina mazei) grown on H2+CO2, acetate, or methanol produced methane with consistently low Δ13CH3D values, down to -5.2‰. Closed system effects can explain part of the non-equilibrium signals for methane from thermophilic methanogens. Experiments with M. barkeri using D-spiked water or D-labeled acetate (CD3COO-) indicate that 1.6 to 1.9 out of four H atoms in methane originate from water, but Δ13CH3D values of product methane only weakly correlate with the D/H ratio of medium water. Our experimental results demonstrate that low Δ13CH3D values are not specific to the metabolic pathways of methanogenesis, suggesting that they could be produced during enzymatic reactions common in the three methanogenic pathways, such as the reduction of methyl-coenzyme M. Nonetheless C-H bonds inherited from precursor methyl groups may also carry part of non-equilibrium signals.
    Description: Grants from the National Science Foundation (EAR-1250394 to S.O.), N. Braunsdorf and D. Smit of Shell PTI/EG (to S.O.), the Deep Carbon Observatory (to S.O., M.K., K.-U.H., D.S.G.), the Gottfried Wilhelm Leibniz Program of the Deutsche Forschungsgemeinschaft (HI 616-14-1 to K.- U.H.), and the Heisenberg Program (KO3651-3-1 to M.K.) of the Deutsche Forschungsgemeinschaft supported this study. D.S.G. was also supported by a National Science Foundation Graduate Research Fellowship, the Neil and Anna Rasmussen Foundation Fund, the Grayce B. Kerr Fellowship, and a Shell-MIT Energy Initiative Graduate Fellowship. D.T.W. was supported by a National Defense Science and Engineering Graduate Fellowship. L.C.S. was supported by a NASA Earth and Space Science Fellowship (grant NNX11AP78H).
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
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  • 9
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    Fractionation of the methane isotopologues 13CH4, 12CH3D, and 13CH3D during aerobic oxidation of methane by Methylococcus capsulatus (Bath) (2016)
    Details
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This is the author's version of the work and is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Geochimica et Cosmochimica Acta 192 (2016): 186–202, doi:10.1016/j.gca.2016.07.031.
    Description: Aerobic oxidation of methane plays a major role in reducing the amount of methane emitted to the atmosphere from freshwater and marine settings. We cultured an aerobic methanotroph, Methylococcus capsulatus (Bath) at 30 and 37 °C, and determined the relative abundance of 12CH4, 13CH4, 12CH3D, and 13CH3D (a doubly-substituted, or “clumped” isotopologue of methane) to characterize the clumped isotopologue effect associated with aerobic methane oxidation. In batch culture, the residual methane became enriched in 13C and D relative to starting methane, with D/H fractionation a factor of 9.14 (Dε/13ε) larger than that of 13C/12C. As oxidation progressed, the Δ13CH3D value (a measure of the excess in abundance of 13CH3D relative to a random distribution of isotopes among isotopologues) of residual methane decreased. The isotopologue fractionation factor for 13CH3D/12CH4 was found to closely approximate the product of the measured fractionation factors for 13CH4/12CH4 and 12CH3D/12CH4 (i.e., 13C/12C and D/H). The results give insight into enzymatic reversibility in the aerobic methane oxidation pathway. Based on the experimental data, a mathematical model was developed to predict isotopologue signatures expected for methane in the environment that has been partially-oxidized by aerobic methanotrophy. Measurement of methane clumped isotopologue abundances can be used to distinguish between aerobic methane oxidation and alternative methane-cycling processes.
    Description: Grants from the National Science Foundation (NSF EAR-1250394 to S.O. and EAR-1451767 to P.V.W.), and the Deep Carbon Observatory (to S.O.) supported this study. S.O. thanks the Kerr-McGee Professorship at MIT. This research was conducted with Government support under and awarded by U.S. Department of Defense, Office of Naval Research, National Defense Science and Engineering Graduate (NDSEG) Fellowship (to D.T.W.), 32 CFR 168a.
    Description: 2017-08-03
    Repository Name: Woods Hole Open Access Server
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  • 10
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    A New Method for Quality Control of Geological Cores by X-Ray Computed Tomography: Application in IODP Expedition 370 (2019)
    In:  EPIC3Frontiers in Earth Science, 7(117)
    Details
    Publication Date: 2021-10-08
    Description: X-ray computed tomography (XCT) can be used to identify lithologies and deformation structures within geological core, with the potential for the identification processes to be applied automatically. However, because of drilling disturbance and other artifacts, the use of large XCT-datasets in automated processes requires methods of quality control that can be applied systematically. We propose a new systematic method for quality control of XCT data that applies numerical measures to CT slices, and from this obtains data reflective of core quality. Because the measures are numerical they can be applied quickly and consistently between different sections and cores. This quality control processing protocol produces downhole radiodensity profiles from mean CT-values that can be used for geological interpretation. The application of this quality control protocols was applied to XCT data from International Ocean Discovery Program (IODP) Expedition 370 Site C0023 located at the toe of the Nankai accretionary complex. The evaluation of core quality based on this protocol was found to be a good fit to standard-evaluations based on the visual description of core, and could be used to select samples free from drilling disturbance or contamination. The quality-controlled downhole mean CT-value profile has features that can be used to identify lithologies within a formation, the presence and type of deformation structures and to distinguish formations.
    Repository Name: EPIC Alfred Wegener Institut
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