ISSN:
1573-5028
Keywords:
ars
;
genome organization
;
maize
;
repeated DNA
;
replication
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Maize nuclear DNA sequences capable of promoting the autonomous replication of plasmids in yeast were isolated by ligating Eco RI-digested fragments into yeast vectors unable to replicate autonomously. Three such autonomously replicating sequences (ARS), representing two families of highly repeated sequences within the maize genome, were isolated and characterized. Each repetitive family shows hybridization patterns on a Southern blot characteristic of a dispersed sequence. Unlike most repetitive sequences in maize, both ARS families have a constant copy number and characteristic genomic hybridization pattern in the inbred lines examined. Larger genome clones with sequence homology to the ARS-containing elements were selected from a lambda library of maize genomic DNA. There was typically only one copy of an ARS-homologous sequence on each 12–15 kb genomic fragment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00015668
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