Publication Date:
1999-05-29
Description:
Bacille Calmette-Guerin (BCG) vaccines are live attenuated strains of Mycobacterium bovis administered to prevent tuberculosis. To better understand the differences between M. tuberculosis, M. bovis, and the various BCG daughter strains, their genomic compositions were studied by performing comparative hybridization experiments on a DNA microarray. Regions deleted from BCG vaccines relative to the virulent M. tuberculosis H37Rv reference strain were confirmed by sequencing across the missing segment of the H37Rv genome. Eleven regions (encompassing 91 open reading frames) of H37Rv were found that were absent from one or more virulent strains of M. bovis. Five additional regions representing 38 open reading frames were present in M. bovis but absent from some or all BCG strains; this is evidence for the ongoing evolution of BCG strains since their original derivation. A precise understanding of the genetic differences between closely related Mycobacteria suggests rational approaches to the design of improved diagnostics and vaccines.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Behr, M A -- Wilson, M A -- Gill, W P -- Salamon, H -- Schoolnik, G K -- Rane, S -- Small, P M -- New York, N.Y. -- Science. 1999 May 28;284(5419):1520-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Infectious Diseases, Department of Medicine, McGill University Health Centre, Montreal H3G 1A4, Canada. mbgq@musica.mcgill.ca〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10348738" target="_blank"〉PubMed〈/a〉
Keywords:
BCG Vaccine/*genetics/immunology
;
DNA, Bacterial/genetics
;
Evolution, Molecular
;
*Gene Deletion
;
Genetic Variation
;
*Genome, Bacterial
;
Humans
;
Mycobacterium bovis/*genetics/immunology/pathogenicity
;
Mycobacterium tuberculosis/*genetics/immunology/pathogenicity
;
Nucleic Acid Hybridization
;
*Oligonucleotide Array Sequence Analysis
;
Open Reading Frames
;
Polymerase Chain Reaction
;
Vaccines, Attenuated
;
Virulence
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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