Publication Date:
2005-11-19
Description:
Nodes of Ranvier are regularly placed, nonmyelinated axon segments along myelinated nerves. Here we show that nodal membranes isolated from the central nervous system (CNS) of mammals restricted neurite outgrowth of cultured neurons. Proteomic analysis of these membranes revealed several inhibitors of neurite outgrowth, including the oligodendrocyte myelin glycoprotein (OMgp). In rat spinal cord, OMgp was not localized to compact myelin, as previously thought, but to oligodendroglia-like cells, whose processes converge to form a ring that completely encircles the nodes. In OMgp-null mice, CNS nodes were abnormally wide and collateral sprouting was observed. Nodal ensheathment in the CNS may stabilize the node and prevent axonal sprouting.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Huang, Jeffrey K -- Phillips, Greg R -- Roth, Alejandro D -- Pedraza, Liliana -- Shan, Weisong -- Belkaid, Wiam -- Mi, Sha -- Fex-Svenningsen, Asa -- Florens, Laurence -- Yates, John R 3rd -- Colman, David R -- NS20147/NS/NINDS NIH HHS/ -- P41 RR11823/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2005 Dec 16;310(5755):1813-7. Epub 2005 Nov 17.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Fishberg Department of Neuroscience, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16293723" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens/analysis
;
Axons/*physiology/ultrastructure
;
Cattle
;
Cell Surface Extensions/chemistry/*physiology/ultrastructure
;
Cells, Cultured
;
GPI-Linked Proteins
;
Ganglia, Spinal/physiology/ultrastructure
;
Humans
;
Mice
;
Myelin Proteins
;
Myelin Sheath/chemistry
;
Myelin-Associated Glycoprotein/analysis
;
Myelin-Oligodendrocyte Glycoprotein
;
Neurites/*physiology/ultrastructure
;
Neuroglia/chemistry/*physiology/*ultrastructure
;
Oligodendroglia/chemistry/physiology/ultrastructure
;
Proteoglycans/analysis
;
Proteomics
;
Ranvier's Nodes/chemistry/*physiology/ultrastructure
;
Rats
;
Spinal Cord/cytology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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