ISSN:
1439-0523
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
The efficiency of marker-assisted selection for oleic acid content was studied in an F2 population of spring turnip rape, Brassica rapa ssp. oleifera, using a sequence characterized amplified region (SCAR) marker, the distance of which from the oleic acid gene is 11.5 cM, and allele-specific markers which are located at the gene locus. As expected, the allele-specific markers recognized the oleic acid genotypes more precisely than the SCAR marker. A further complication in the use of the SCAR was the existence of null alleles and polymorphism in almost all the lines/cultivars tested. Therefore, parents have to be carefully selected to find informative combinations in different crosses. Allele-specific markers are very efficient in selection since heterozygous plants can be identified. This is not possible when selection is based on the commonly applied gas chromatography analysis of oil composition. The allele-specific detection was further simplified by using direct staining of PCR products instead of electrophoresis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1439-0523.1999.00425.x
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