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  • 1
    Electronic Resource
    Electronic Resource
    Allozyme, chloroplast DNA and RAPD markers for determining genetic relationships between Abies alba and the relic population of Abies nebrodensis (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1012-1018 
    Details
    ISSN: 1432-2242
    Keywords: Allozymes ; Chloroplast DNA ; RAPD ; Genetic variation ; Abies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Allozyme, chloroplast (cpDNA) and random amplified polymorphic DNA (RAPD) markers have been used to estimate genetic and taxonomic relationships among different populations of Abies alba and the relic population of A. nebrodensis. Twelve isozyme gene loci, as well as restriction fragment length polymorphism (RFLP) at cpDNA spacer regions between t-RNA genes were analysed. Moreover, a set of 60 random sequence 10-mer primers were tested. Over all isozyme loci, evident differences in allele frequencies among A. nebrodensis and A. alba populations were found, particularly at 2 loci, phosphoglucose isomerase (Pgi-a) and shikimate dehydrogenase (Skd-a). More than 10% of the total genetic diversity was due to differences among populations. High values of genetic distances among populations were also found. Out of the 60 primers tested, 12 resulted in a polymorphic banding pattern both within and among populations. A total of 84 RAPD fragments were produced by the 12 selected primers. A phenogram of relationships among populations was constructed based on RAPD band sharing: the differentiation of the A. nebrodensis population was evident. The analysis of molecular variance (AMOVA) was used to apportion the variation among individuals within populations and among populations. There was considerable variation within each population: even so, genetic divergence was found among populations. This pattern of genetic variation was very different from that reported for inbred species. Identical cpDNA amplification and restriction patterns were observed among all the individuals sampled from the populations. Taken together, the results of allozyme and RAPDs show a clear differentiation among A. nebrodensis and A. alba populations and provide support for their classification into two different taxonomic groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222915
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  • 2
    Electronic Resource
    Electronic Resource
    Assessing the genetic divergence of Pinus leucodermis Ant. endangered populations: use of molecular markers for conservation purposes (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 1138-1146 
    Details
    ISSN: 1432-2242
    Keywords: Key words Allozymes ; RAPDs ; Genetic differentiation ; Linkage disequilibrium ; Population genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In this study, 23 previously identified Mendelian RAPD markers and 16 polymorphic allozymic markers were used to assess divergence among two Greek populations and five Italian populations of Pinus leucodermis. Confidence intervals of observed genetic divergence were obtained using bootstrap analysis. Divergence among Italian populations was found to be about as large as that between Italian and Greek populations. Since it is likely that the split of two nuclei took place more than 10,000 years ago, a larger differentiation between, rather than within, the above nuclei was expected. If genetic drift was responsible for the larger divergence of Italian populations, large randomly generated disequilibrium between alleles at neutral, unlinked loci was expected. Indeed, the proportion of pairs of loci showing a non-random association of alleles within each of the Italian populations was larger than what was expected by pure chance (7.95–10.88%). Effective population size based on randomly generated disequilibrium was quite small for three out of the five populations considered (N e =17.31±1.88, 16.57± 1.73, and 31.41±7.26, respectively). The implications of the results with respect to the conservation of endangered species of trees are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050674
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  • 3
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    Attosecond Control in Photoionization of Hydrogen Molecules (2011)
    American Physical Society (APS)
    Details
    Publication Date: 2011-07-20
    Description: Author(s): F. Kelkensberg, W. Siu, J. F. Pérez-Torres, F. Morales, G. Gademann, A. Rouzée, P. Johnsson, M. Lucchini, F. Calegari, J. L. Sanz-Vicario, F. Martín, and M. J. J. Vrakking We report experiments where hydrogen molecules were dissociatively ionized by an attosecond pulse train in the presence of a near-infrared field. Fragment ion yields from distinguishable ionization channels oscillate with a period that is half the optical cycle of the IR field. For molecules aligned... [Phys. Rev. Lett. 107, 043002] Published Tue Jul 19, 2011
    Keywords: Atomic, Molecular, and Optical Physics
    Print ISSN: 0031-9007
    Electronic ISSN: 1079-7114
    Topics: Physics
    Published by American Physical Society (APS)
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