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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 57 (1983), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In chlorophylkras soybean (Glycine max L.) cell suspensioo cultures glucose uptake has been studied using the analogue 3-O-methyIglucose. Uptake could be distinguished into: a) a high affinity phase with Km= 0.06 mM and b) a low affinity phase with Km 2.0 mM. The uptake of glucose was accompanied by H+-cotransport with a stoichiometry of 0.3 H+ per molecule 3-O-methylglucose. Experiments in which sugar uptake was measured in the presence of various inhibitors of respiration and photosynthesis demonstrated that the glucose uptake system was dependent on energy metabolism and the ATP-content of the cells. Efflux experiments in the presence of the uncoupler dinitrophenol confirmed this energy dependency. Glucose uptake did not decrease before the ATP-content of the cells had decreased considerably.
    Type of Medium: Electronic Resource
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